GSE8896 : Flagellin-mediated effects of Salmonella enteritidis on gene expression in intestinal epithelial cells (GSE8896)

  • Denise Kelly (Creator)
  • Imke Elisabeth Mulder (Creator)
  • Peter John Morgan (Data Manager)

Dataset

Description

Experiment type: Expression profiling by array

Analysis of gene expression in Caco-2 intestinal epithelial cells stimulated with flagellated or aflagellated S. enteritidis or recombinant flagellin. Results provide insight into how flagellin mediates innate immune responses in intestinal epithelial cells.

Overall design: Caco-2 cells were grown in 100 mm dishes to 3 days post-confluence. Cells were then incubated for 2 h with the following four treatments: medium alone (control); flagellated S. enteritidis; aflagellated S. enteritidis; or recombinant flagellin. Three independent experiments were performed to give an N=3 for each treatment. Caco-2 RNA was extracted and hybridized to the Affymetrix GeneChip Human Genome U133 Plus 2.0 Array

Keywords: Gene expression analysis
Date made available5 Feb 2016
PublisherGEO
Date of data production29 Aug 2007

Cite this

Kelly, D. (Creator), Mulder, I. E. (Creator), Morgan, P. J. (Data Manager)(5 Feb 2016). GSE8896 : Flagellin-mediated effects of Salmonella enteritidis on gene expression in intestinal epithelial cells (GSE8896). GEO.