Rapid and extensive karyotype diversification in haploid clinical Candida auris isolates

Gustavo Bravo Ruiz; Zoe K. Ross; Silke Schelenz; Neil A. R. Gow; Alexander Lorenz

doi:10.1101/514745

Rapid and extensive karyotype diversification in haploid clinical Candida auris isolates

Gustavo Bravo Ruiz, Zoe K. Ross, Silke Schelenz, Neil A. R. Gow, Alexander Lorenz^* (Corresponding Author)

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

38 Citations (Scopus)

24 Downloads (Pure)

Abstract

Candida auris is a newly emerging pathogenic microbe, having been identified as a medically relevant fungus as recently as 2009. It is the most drug-resistant yeast species known to date and its emergence and population structure are unusual. Because of its recent emergence we are largely ignorant about fundamental aspects of its general biology, life cycle, and population dynamics. Here we report the karyotype variability of 26 C. auris strains representing the four main clades. We demonstrate that all strains are haploid and have a highly plastic karyotype containing five to seven chromosomes, which can undergo marked alterations within a short time-frame when the fungus is put under genotoxic, heat, or osmotic stress. No simple correlation was found between karyotype pattern, drug resistance, and clade affiliation indicating that karyotype heterogeneity is rapidly evolving. As with other Candida species, these marked karyotype differences between isolates are likely to have an important impact on pathogenic traits of C. auris.

Original language	English
Pages (from-to)	1217-1228
Number of pages	12
Journal	Current Genetics
Volume	65
Issue number	5
Early online date	24 Apr 2019
DOIs	https://doi.org/10.1101/514745 https://doi.org/10.1007/s00294-019-00976-w
Publication status	Published - Oct 2019

Bibliographical note

Open access via the Springer Compact Agreement.
We are grateful to Arunaloke Chakrabarti, Anuradha Chowdhary, Elizabeth Johnson (PHE), Takashi Kubota, and Shawn Lockhart (CDC) for providing strains. Flow cytometry was performed at the Iain Fraser Cytometry Centre (IFCC), University of Aberdeen (Raif Yuecel). This work was funded by the Medical Research Council (MRC) Centre for Medical Mycology at the University of Aberdeen (MR/P501955/1), a Wellcome Trust Institutional Strategic Support Fund grant awarded to the University of Aberdeen (204815/Z/16/Z), a Tenovus Scotland project grant (G17.02), a Royal Society Research Grant (RG140254) to AL, and Wellcome Trust Strategic Award, Senior Investigator and Collaborative Awards (080088, 086827, 075470, 099215, and 097377) to NARG.

Keywords

Candida auris
chromosome number
chromosome size
genome size
rRNA genes
Genome size
Chromosome size
Chromosome number
Karyotype evolution
PHENOTYPIC VARIATION
DE-NOVO
NUMBER
INSTABILITY
MECHANISM
GENOME
ANEUPLOIDY
MUTANTS
RIBOSOMAL DNA
ALBICANS

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10.1101/514745Licence: CC BY-NC-ND
10.1007/s00294-019-00976-wLicence: CC BY

Rapid and extensive karyotype diversification in haploid clinical Candida auris isolates
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Submitted manuscript, 1.1 MBLicence: CC BY-NC-ND
BravoRuiz2019_Article_RapidAndExtensiveKaryotypeDive_VOR
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Final published version, 1.29 MBLicence: CC BY

Cite this

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title = "Rapid and extensive karyotype diversification in haploid clinical Candida auris isolates",

abstract = "Candida auris is a newly emerging pathogenic microbe, having been identified as a medically relevant fungus as recently as 2009. It is the most drug-resistant yeast species known to date and its emergence and population structure are unusual. Because of its recent emergence we are largely ignorant about fundamental aspects of its general biology, life cycle, and population dynamics. Here we report the karyotype variability of 26 C. auris strains representing the four main clades. We demonstrate that all strains are haploid and have a highly plastic karyotype containing five to seven chromosomes, which can undergo marked alterations within a short time-frame when the fungus is put under genotoxic, heat, or osmotic stress. No simple correlation was found between karyotype pattern, drug resistance, and clade affiliation indicating that karyotype heterogeneity is rapidly evolving. As with other Candida species, these marked karyotype differences between isolates are likely to have an important impact on pathogenic traits of C. auris.",

keywords = "Candida auris, chromosome number, chromosome size, genome size, rRNA genes, Genome size, Chromosome size, Chromosome number, Karyotype evolution, PHENOTYPIC VARIATION, DE-NOVO, NUMBER, INSTABILITY, MECHANISM, GENOME, ANEUPLOIDY, MUTANTS, RIBOSOMAL DNA, ALBICANS",

author = "{Bravo Ruiz}, Gustavo and Ross, {Zoe K.} and Silke Schelenz and Gow, {Neil A. R.} and Alexander Lorenz",

note = "Open access via the Springer Compact Agreement. We are grateful to Arunaloke Chakrabarti, Anuradha Chowdhary, Elizabeth Johnson (PHE), Takashi Kubota, and Shawn Lockhart (CDC) for providing strains. Flow cytometry was performed at the Iain Fraser Cytometry Centre (IFCC), University of Aberdeen (Raif Yuecel). This work was funded by the Medical Research Council (MRC) Centre for Medical Mycology at the University of Aberdeen (MR/P501955/1), a Wellcome Trust Institutional Strategic Support Fund grant awarded to the University of Aberdeen (204815/Z/16/Z), a Tenovus Scotland project grant (G17.02), a Royal Society Research Grant (RG140254) to AL, and Wellcome Trust Strategic Award, Senior Investigator and Collaborative Awards (080088, 086827, 075470, 099215, and 097377) to NARG. ",

year = "2019",

month = oct,

doi = "10.1101/514745",

language = "English",

volume = "65",

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journal = "Current Genetics",

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AU - Bravo Ruiz, Gustavo

AU - Ross, Zoe K.

AU - Schelenz, Silke

AU - Gow, Neil A. R.

AU - Lorenz, Alexander

N1 - Open access via the Springer Compact Agreement. We are grateful to Arunaloke Chakrabarti, Anuradha Chowdhary, Elizabeth Johnson (PHE), Takashi Kubota, and Shawn Lockhart (CDC) for providing strains. Flow cytometry was performed at the Iain Fraser Cytometry Centre (IFCC), University of Aberdeen (Raif Yuecel). This work was funded by the Medical Research Council (MRC) Centre for Medical Mycology at the University of Aberdeen (MR/P501955/1), a Wellcome Trust Institutional Strategic Support Fund grant awarded to the University of Aberdeen (204815/Z/16/Z), a Tenovus Scotland project grant (G17.02), a Royal Society Research Grant (RG140254) to AL, and Wellcome Trust Strategic Award, Senior Investigator and Collaborative Awards (080088, 086827, 075470, 099215, and 097377) to NARG.

PY - 2019/10

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N2 - Candida auris is a newly emerging pathogenic microbe, having been identified as a medically relevant fungus as recently as 2009. It is the most drug-resistant yeast species known to date and its emergence and population structure are unusual. Because of its recent emergence we are largely ignorant about fundamental aspects of its general biology, life cycle, and population dynamics. Here we report the karyotype variability of 26 C. auris strains representing the four main clades. We demonstrate that all strains are haploid and have a highly plastic karyotype containing five to seven chromosomes, which can undergo marked alterations within a short time-frame when the fungus is put under genotoxic, heat, or osmotic stress. No simple correlation was found between karyotype pattern, drug resistance, and clade affiliation indicating that karyotype heterogeneity is rapidly evolving. As with other Candida species, these marked karyotype differences between isolates are likely to have an important impact on pathogenic traits of C. auris.

AB - Candida auris is a newly emerging pathogenic microbe, having been identified as a medically relevant fungus as recently as 2009. It is the most drug-resistant yeast species known to date and its emergence and population structure are unusual. Because of its recent emergence we are largely ignorant about fundamental aspects of its general biology, life cycle, and population dynamics. Here we report the karyotype variability of 26 C. auris strains representing the four main clades. We demonstrate that all strains are haploid and have a highly plastic karyotype containing five to seven chromosomes, which can undergo marked alterations within a short time-frame when the fungus is put under genotoxic, heat, or osmotic stress. No simple correlation was found between karyotype pattern, drug resistance, and clade affiliation indicating that karyotype heterogeneity is rapidly evolving. As with other Candida species, these marked karyotype differences between isolates are likely to have an important impact on pathogenic traits of C. auris.

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KW - rRNA genes

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KW - PHENOTYPIC VARIATION

KW - DE-NOVO

KW - NUMBER

KW - INSTABILITY

KW - MECHANISM

KW - GENOME

KW - ANEUPLOIDY

KW - MUTANTS

KW - RIBOSOMAL DNA

KW - ALBICANS

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SN - 0172-8083

VL - 65

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JO - Current Genetics

JF - Current Genetics

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ER -

Rapid and extensive karyotype diversification in haploid clinical Candida auris isolates

Abstract

Bibliographical note

Keywords

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Alexander Lorenz

Iain Fraser Cytometry Centre

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Rapid and extensive karyotype diversification in haploid clinical Candida auris isolates

Abstract

Bibliographical note

Keywords

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Profiles

Alexander Lorenz

Equipment

Iain Fraser Cytometry Centre

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