99mTc-labelled human serum transferrin for tumour imaging: an in vitro and in vivo study of the complex.

Timothy Andrew Davies Smith, P. H. Walton, A. Perkins

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

This pilot study reports the uptake of Tc-99m-transferrin by MCF7 cells and the biodistribution of the complex in mice bearing MCF7 tumours. Human serum holo-transferrin was labelled with Tc-99m using pre-reduction with 2-mercaptoethanol. The uptake of the complex by MCF7 breast tumour cells, which was found to be competitively inhibited by the presence of unlabelled transferrin, reached a plateau within 30 min. Two groups of xenografted mice with small and large tumours, respectively, were injected with the complex, and the uptake was followed for up to 24 h post-administration using a dedicated gamma camera. The mean tumour uptake values, determined after dissection, in the two groups of mice were 6% (small tumours) and 1.7% (large tumours) of the injected dose per gram of tissue, with mean tumour/blood ratios of 2.7 and 1.7, respectively. (C) 2004 Lippincott Williams Wilkins.

Original languageEnglish
Pages (from-to)387-391
Number of pages4
JournalNuclear Medicine Communications
Volume25
DOIs
Publication statusPublished - 2004

Keywords

  • Tc-99m-transferrin
  • tumour targeting
  • biodistribution
  • RECEPTOR EXPRESSION
  • BINDING
  • ANALOG
  • CELLS
  • ENDOCYTOSIS
  • PROTEINS
  • CANCER
  • TC-99M

Cite this

99mTc-labelled human serum transferrin for tumour imaging: an in vitro and in vivo study of the complex. / Smith, Timothy Andrew Davies; Walton, P. H.; Perkins, A.

In: Nuclear Medicine Communications, Vol. 25, 2004, p. 387-391.

Research output: Contribution to journalArticle

Smith, Timothy Andrew Davies ; Walton, P. H. ; Perkins, A. / 99mTc-labelled human serum transferrin for tumour imaging: an in vitro and in vivo study of the complex. In: Nuclear Medicine Communications. 2004 ; Vol. 25. pp. 387-391.
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AB - This pilot study reports the uptake of Tc-99m-transferrin by MCF7 cells and the biodistribution of the complex in mice bearing MCF7 tumours. Human serum holo-transferrin was labelled with Tc-99m using pre-reduction with 2-mercaptoethanol. The uptake of the complex by MCF7 breast tumour cells, which was found to be competitively inhibited by the presence of unlabelled transferrin, reached a plateau within 30 min. Two groups of xenografted mice with small and large tumours, respectively, were injected with the complex, and the uptake was followed for up to 24 h post-administration using a dedicated gamma camera. The mean tumour uptake values, determined after dissection, in the two groups of mice were 6% (small tumours) and 1.7% (large tumours) of the injected dose per gram of tissue, with mean tumour/blood ratios of 2.7 and 1.7, respectively. (C) 2004 Lippincott Williams Wilkins.

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KW - tumour targeting

KW - biodistribution

KW - RECEPTOR EXPRESSION

KW - BINDING

KW - ANALOG

KW - CELLS

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KW - PROTEINS

KW - CANCER

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