TY - JOUR
T1 - A β-glucan-conjugate vaccine and anti-β-glucan antibodies are effective against murine vaginal candidiasis as assessed by a novel in vivo imaging technique
AU - Pietrella, Donatella
AU - Rachini, Anna
AU - Torosantucci, Antonella
AU - Chiani, Paola
AU - Brown, Alistair J.P.
AU - Bistoni, Francesco
AU - Costantino, Paolo
AU - Mosci, Paolo
AU - d'Enfert, Christophe
AU - Rappuoli, Rino
AU - Cassone, Antonio
AU - Vecchiarelli, Anna
PY - 2010/2/17
Y1 - 2010/2/17
N2 - The protective capacity of a parenterally administered β-glucan-conjugate vaccine formulated with the human-compatible MF59 adjuvant was assessed in a murine model of vaginal candidiasis. To monitor infection, an in vivo imaging technique exploiting genetically engineered, luminescent Candida albicans was adopted, and compared with measurements of colony forming units. The vaccine conferred significant protection, and this was associated with production of serum and vaginal anti-β-glucan IgG antibodies. Vaginal IgG molecules were the likely mediators of protection as inferred by the efficacy of passive transfer of immune vaginal fluid and passive protection by an anti-β-1,3-glucan mAb. Overall, the in vivo imaging technique was more reliable than vaginal CFU counts in assessing the extent and duration of the vaginal infection, and the consequent protection level.
AB - The protective capacity of a parenterally administered β-glucan-conjugate vaccine formulated with the human-compatible MF59 adjuvant was assessed in a murine model of vaginal candidiasis. To monitor infection, an in vivo imaging technique exploiting genetically engineered, luminescent Candida albicans was adopted, and compared with measurements of colony forming units. The vaccine conferred significant protection, and this was associated with production of serum and vaginal anti-β-glucan IgG antibodies. Vaginal IgG molecules were the likely mediators of protection as inferred by the efficacy of passive transfer of immune vaginal fluid and passive protection by an anti-β-1,3-glucan mAb. Overall, the in vivo imaging technique was more reliable than vaginal CFU counts in assessing the extent and duration of the vaginal infection, and the consequent protection level.
KW - Candida
KW - In vivo imaging
KW - MF59
KW - Vaginal infection
UR - http://www.scopus.com/inward/record.url?scp=75249095379&partnerID=8YFLogxK
U2 - 10.1016/j.vaccine.2009.12.021
DO - 10.1016/j.vaccine.2009.12.021
M3 - Article
C2 - 20038431
AN - SCOPUS:75249095379
SN - 0264-410X
VL - 28
SP - 1717
EP - 1725
JO - Vaccine
JF - Vaccine
IS - 7
ER -