A biologically active IL-1 beta derived peptide stimulates phagocytosis and bactericidal activity in rainbow trout, Oncorhynchus mykiss (Walbaum), head kidney leucocytes in vitro

S Peddie, Jun Zou, Christopher John Secombes

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The present work provides the first information concerning the ability of a rainbow trout interleukin-1beta (IL-1beta) derived peptide, P3, to enhance phagocytosis and bactericidal activity. P3 was synthesized to complex with the IL-1 receptor and corresponds to fragment 197-206 (YRRNTGVDIS) of the trout sequence. A dose-dependent effect of P3 on phagocytosis in head kidney leucocytes was documented, with an optimal response occurring at 0.25 mM. With respect to the temporal kinetics of P3 induced activation, an incubation period of 2 h resulted in enhanced phagocytic activity, although incubation times of between 4 and 24 h were optimal. An additive effect was observed when P3 (0.1 mM) was combined with another IL-1 derived peptide, P1 (0.1 mM). The latter peptide corresponds to fragment 146-157 (YVTPVPIETEAR) and is equivalent to a region in the mammalian protein that is known to be part of the receptor binding domain, but does not overlap with P3. With respect to bactericidal activity, macrophages incubated for 24 h with 0.1 mM P3 demonstrated an enhanced ability to kill an avirulent strain (MT004) of the Gram-negative salmonid pathogen Aeromonas salmonicida . Further investigation revealed that extracellular production of superoxide anions was not responsible for the enhanced bactericidal activity of macrophages exposed to P3.

Original languageEnglish
Pages (from-to)351-360
Number of pages10
JournalJournal of Fish Diseases
Volume25
Issue number6
DOIs
Publication statusPublished - Jun 2002

Keywords

  • bactericidal activity
  • immunomodulation
  • interleukin-1 beta
  • leucocytes
  • peptides
  • phagocytosis
  • rainbow trout
  • recombinant protein
  • respiratory burst
  • aprus-aurata L.
  • in-vitro
  • molecular-cloning
  • interleukin-1 receptor
  • aeromonas-salmonicida
  • recombinant IL-1-beta
  • expression analysis
  • vitamin-E
  • leukocytes
  • enhancement

Cite this

@article{2c67bf9d3d2544a6bfb330d6353d6494,
title = "A biologically active IL-1 beta derived peptide stimulates phagocytosis and bactericidal activity in rainbow trout, Oncorhynchus mykiss (Walbaum), head kidney leucocytes in vitro",
abstract = "The present work provides the first information concerning the ability of a rainbow trout interleukin-1beta (IL-1beta) derived peptide, P3, to enhance phagocytosis and bactericidal activity. P3 was synthesized to complex with the IL-1 receptor and corresponds to fragment 197-206 (YRRNTGVDIS) of the trout sequence. A dose-dependent effect of P3 on phagocytosis in head kidney leucocytes was documented, with an optimal response occurring at 0.25 mM. With respect to the temporal kinetics of P3 induced activation, an incubation period of 2 h resulted in enhanced phagocytic activity, although incubation times of between 4 and 24 h were optimal. An additive effect was observed when P3 (0.1 mM) was combined with another IL-1 derived peptide, P1 (0.1 mM). The latter peptide corresponds to fragment 146-157 (YVTPVPIETEAR) and is equivalent to a region in the mammalian protein that is known to be part of the receptor binding domain, but does not overlap with P3. With respect to bactericidal activity, macrophages incubated for 24 h with 0.1 mM P3 demonstrated an enhanced ability to kill an avirulent strain (MT004) of the Gram-negative salmonid pathogen Aeromonas salmonicida . Further investigation revealed that extracellular production of superoxide anions was not responsible for the enhanced bactericidal activity of macrophages exposed to P3.",
keywords = "bactericidal activity, immunomodulation, interleukin-1 beta, leucocytes, peptides, phagocytosis, rainbow trout, recombinant protein, respiratory burst, aprus-aurata L., in-vitro, molecular-cloning, interleukin-1 receptor, aeromonas-salmonicida, recombinant IL-1-beta, expression analysis, vitamin-E, leukocytes, enhancement",
author = "S Peddie and Jun Zou and Secombes, {Christopher John}",
year = "2002",
month = "6",
doi = "10.1046/j.1365-2761.2002.00380.x",
language = "English",
volume = "25",
pages = "351--360",
journal = "Journal of Fish Diseases",
issn = "0140-7775",
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TY - JOUR

T1 - A biologically active IL-1 beta derived peptide stimulates phagocytosis and bactericidal activity in rainbow trout, Oncorhynchus mykiss (Walbaum), head kidney leucocytes in vitro

AU - Peddie, S

AU - Zou, Jun

AU - Secombes, Christopher John

PY - 2002/6

Y1 - 2002/6

N2 - The present work provides the first information concerning the ability of a rainbow trout interleukin-1beta (IL-1beta) derived peptide, P3, to enhance phagocytosis and bactericidal activity. P3 was synthesized to complex with the IL-1 receptor and corresponds to fragment 197-206 (YRRNTGVDIS) of the trout sequence. A dose-dependent effect of P3 on phagocytosis in head kidney leucocytes was documented, with an optimal response occurring at 0.25 mM. With respect to the temporal kinetics of P3 induced activation, an incubation period of 2 h resulted in enhanced phagocytic activity, although incubation times of between 4 and 24 h were optimal. An additive effect was observed when P3 (0.1 mM) was combined with another IL-1 derived peptide, P1 (0.1 mM). The latter peptide corresponds to fragment 146-157 (YVTPVPIETEAR) and is equivalent to a region in the mammalian protein that is known to be part of the receptor binding domain, but does not overlap with P3. With respect to bactericidal activity, macrophages incubated for 24 h with 0.1 mM P3 demonstrated an enhanced ability to kill an avirulent strain (MT004) of the Gram-negative salmonid pathogen Aeromonas salmonicida . Further investigation revealed that extracellular production of superoxide anions was not responsible for the enhanced bactericidal activity of macrophages exposed to P3.

AB - The present work provides the first information concerning the ability of a rainbow trout interleukin-1beta (IL-1beta) derived peptide, P3, to enhance phagocytosis and bactericidal activity. P3 was synthesized to complex with the IL-1 receptor and corresponds to fragment 197-206 (YRRNTGVDIS) of the trout sequence. A dose-dependent effect of P3 on phagocytosis in head kidney leucocytes was documented, with an optimal response occurring at 0.25 mM. With respect to the temporal kinetics of P3 induced activation, an incubation period of 2 h resulted in enhanced phagocytic activity, although incubation times of between 4 and 24 h were optimal. An additive effect was observed when P3 (0.1 mM) was combined with another IL-1 derived peptide, P1 (0.1 mM). The latter peptide corresponds to fragment 146-157 (YVTPVPIETEAR) and is equivalent to a region in the mammalian protein that is known to be part of the receptor binding domain, but does not overlap with P3. With respect to bactericidal activity, macrophages incubated for 24 h with 0.1 mM P3 demonstrated an enhanced ability to kill an avirulent strain (MT004) of the Gram-negative salmonid pathogen Aeromonas salmonicida . Further investigation revealed that extracellular production of superoxide anions was not responsible for the enhanced bactericidal activity of macrophages exposed to P3.

KW - bactericidal activity

KW - immunomodulation

KW - interleukin-1 beta

KW - leucocytes

KW - peptides

KW - phagocytosis

KW - rainbow trout

KW - recombinant protein

KW - respiratory burst

KW - aprus-aurata L.

KW - in-vitro

KW - molecular-cloning

KW - interleukin-1 receptor

KW - aeromonas-salmonicida

KW - recombinant IL-1-beta

KW - expression analysis

KW - vitamin-E

KW - leukocytes

KW - enhancement

U2 - 10.1046/j.1365-2761.2002.00380.x

DO - 10.1046/j.1365-2761.2002.00380.x

M3 - Article

VL - 25

SP - 351

EP - 360

JO - Journal of Fish Diseases

JF - Journal of Fish Diseases

SN - 0140-7775

IS - 6

ER -