Abstract
The present work provides the first information concerning the ability of a rainbow trout interleukin-1beta (IL-1beta) derived peptide, P3, to enhance phagocytosis and bactericidal activity. P3 was synthesized to complex with the IL-1 receptor and corresponds to fragment 197-206 (YRRNTGVDIS) of the trout sequence. A dose-dependent effect of P3 on phagocytosis in head kidney leucocytes was documented, with an optimal response occurring at 0.25 mM. With respect to the temporal kinetics of P3 induced activation, an incubation period of 2 h resulted in enhanced phagocytic activity, although incubation times of between 4 and 24 h were optimal. An additive effect was observed when P3 (0.1 mM) was combined with another IL-1 derived peptide, P1 (0.1 mM). The latter peptide corresponds to fragment 146-157 (YVTPVPIETEAR) and is equivalent to a region in the mammalian protein that is known to be part of the receptor binding domain, but does not overlap with P3. With respect to bactericidal activity, macrophages incubated for 24 h with 0.1 mM P3 demonstrated an enhanced ability to kill an avirulent strain (MT004) of the Gram-negative salmonid pathogen Aeromonas salmonicida . Further investigation revealed that extracellular production of superoxide anions was not responsible for the enhanced bactericidal activity of macrophages exposed to P3.
| Original language | English |
|---|---|
| Pages (from-to) | 351-360 |
| Number of pages | 10 |
| Journal | Journal of Fish Diseases |
| Volume | 25 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Jun 2002 |
Keywords
- bactericidal activity
- immunomodulation
- interleukin-1 beta
- leucocytes
- peptides
- phagocytosis
- rainbow trout
- recombinant protein
- respiratory burst
- aprus-aurata L.
- in-vitro
- molecular-cloning
- interleukin-1 receptor
- aeromonas-salmonicida
- recombinant IL-1-beta
- expression analysis
- vitamin-E
- leukocytes
- enhancement
Cite this
A biologically active IL-1 beta derived peptide stimulates phagocytosis and bactericidal activity in rainbow trout, Oncorhynchus mykiss (Walbaum), head kidney leucocytes in vitro. / Peddie, S ; Zou, Jun; Secombes, Christopher John.
In: Journal of Fish Diseases, Vol. 25, No. 6, 06.2002, p. 351-360.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A biologically active IL-1 beta derived peptide stimulates phagocytosis and bactericidal activity in rainbow trout, Oncorhynchus mykiss (Walbaum), head kidney leucocytes in vitro
AU - Peddie, S
AU - Zou, Jun
AU - Secombes, Christopher John
PY - 2002/6
Y1 - 2002/6
N2 - The present work provides the first information concerning the ability of a rainbow trout interleukin-1beta (IL-1beta) derived peptide, P3, to enhance phagocytosis and bactericidal activity. P3 was synthesized to complex with the IL-1 receptor and corresponds to fragment 197-206 (YRRNTGVDIS) of the trout sequence. A dose-dependent effect of P3 on phagocytosis in head kidney leucocytes was documented, with an optimal response occurring at 0.25 mM. With respect to the temporal kinetics of P3 induced activation, an incubation period of 2 h resulted in enhanced phagocytic activity, although incubation times of between 4 and 24 h were optimal. An additive effect was observed when P3 (0.1 mM) was combined with another IL-1 derived peptide, P1 (0.1 mM). The latter peptide corresponds to fragment 146-157 (YVTPVPIETEAR) and is equivalent to a region in the mammalian protein that is known to be part of the receptor binding domain, but does not overlap with P3. With respect to bactericidal activity, macrophages incubated for 24 h with 0.1 mM P3 demonstrated an enhanced ability to kill an avirulent strain (MT004) of the Gram-negative salmonid pathogen Aeromonas salmonicida . Further investigation revealed that extracellular production of superoxide anions was not responsible for the enhanced bactericidal activity of macrophages exposed to P3.
AB - The present work provides the first information concerning the ability of a rainbow trout interleukin-1beta (IL-1beta) derived peptide, P3, to enhance phagocytosis and bactericidal activity. P3 was synthesized to complex with the IL-1 receptor and corresponds to fragment 197-206 (YRRNTGVDIS) of the trout sequence. A dose-dependent effect of P3 on phagocytosis in head kidney leucocytes was documented, with an optimal response occurring at 0.25 mM. With respect to the temporal kinetics of P3 induced activation, an incubation period of 2 h resulted in enhanced phagocytic activity, although incubation times of between 4 and 24 h were optimal. An additive effect was observed when P3 (0.1 mM) was combined with another IL-1 derived peptide, P1 (0.1 mM). The latter peptide corresponds to fragment 146-157 (YVTPVPIETEAR) and is equivalent to a region in the mammalian protein that is known to be part of the receptor binding domain, but does not overlap with P3. With respect to bactericidal activity, macrophages incubated for 24 h with 0.1 mM P3 demonstrated an enhanced ability to kill an avirulent strain (MT004) of the Gram-negative salmonid pathogen Aeromonas salmonicida . Further investigation revealed that extracellular production of superoxide anions was not responsible for the enhanced bactericidal activity of macrophages exposed to P3.
KW - bactericidal activity
KW - immunomodulation
KW - interleukin-1 beta
KW - leucocytes
KW - peptides
KW - phagocytosis
KW - rainbow trout
KW - recombinant protein
KW - respiratory burst
KW - aprus-aurata L.
KW - in-vitro
KW - molecular-cloning
KW - interleukin-1 receptor
KW - aeromonas-salmonicida
KW - recombinant IL-1-beta
KW - expression analysis
KW - vitamin-E
KW - leukocytes
KW - enhancement
U2 - 10.1046/j.1365-2761.2002.00380.x
DO - 10.1046/j.1365-2761.2002.00380.x
M3 - Article
VL - 25
SP - 351
EP - 360
JO - Journal of Fish Diseases
JF - Journal of Fish Diseases
SN - 0140-7775
IS - 6
ER -