A Candida albicans genome project: Cosmid contigs, physical mapping, and gene isolation

Evelyn Tait; Moyra C. Simon; Neil Andrew Robert Gow; Alistair James Petersen Brown; Duncan James Shaw; Susan  King

doi:10.1006/fgbi.1997.0983

A Candida albicans genome project: Cosmid contigs, physical mapping, and gene isolation

Evelyn Tait, Moyra C. Simon, Neil Andrew Robert Gow, Alistair James Petersen Brown, Duncan James Shaw, Susan King

University of Aberdeen

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

Abstract

A new project to map the genome of the pathogenic fungus, Candida albicans, has been started. The entire genome was cloned as 5088 cosmids, stored in individual microtiter plate wells. DNA was prepared and fingerprinted using restriction digestion, fluorescent labeling, and analysis on an ABI sequencer. These data are being used to construct contigs of the genome. Simultaneously, a DNA pooling system has been set up, suitable for PCR-based isolation of cosmids containing any known gene. Ultimately, these approaches will lead to the creation of a physically based map of the C. albicans genome, providing the means to localize precisely all the genes, act as a substrate for genome sequencing projects, and provide probes for future studies of genome rearrangement and comparative genomics.

Original language	English
Pages (from-to)	308-314
Number of pages	7
Journal	Fungal Genetics and Biology
Volume	21
Issue number	3
DOIs	https://doi.org/10.1006/fgbi.1997.0983
Publication status	Published - 1 Jan 1997

Bibliographical note

These studies are supported by a project grant from the Wellcome Trust. We thank colleagues in our laboratory for providing primer sequences for PCR.

Keywords

Albicans
Candida
Contigs
Cosmids
Fungus
Genetics
Genome
Genomics
Map
Yeast

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10.1006/fgbi.1997.0983

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abstract = "A new project to map the genome of the pathogenic fungus, Candida albicans, has been started. The entire genome was cloned as 5088 cosmids, stored in individual microtiter plate wells. DNA was prepared and fingerprinted using restriction digestion, fluorescent labeling, and analysis on an ABI sequencer. These data are being used to construct contigs of the genome. Simultaneously, a DNA pooling system has been set up, suitable for PCR-based isolation of cosmids containing any known gene. Ultimately, these approaches will lead to the creation of a physically based map of the C. albicans genome, providing the means to localize precisely all the genes, act as a substrate for genome sequencing projects, and provide probes for future studies of genome rearrangement and comparative genomics.",

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note = "These studies are supported by a project grant from the Wellcome Trust. We thank colleagues in our laboratory for providing primer sequences for PCR.",

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AU - Brown, Alistair James Petersen

AU - Shaw, Duncan James

AU - King, Susan

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A Candida albicans genome project: Cosmid contigs, physical mapping, and gene isolation

Abstract

Bibliographical note

Keywords

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