A catalytic hydropyrolysis method for the rapid screening of microbial cultures for lipid biomarkers

G. D. Love, Stephen Alan Bowden, L. L. Jahnke, C. E. Snape, C. N. Campbell, D. Day, R. E. Summons

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

A catalytic hydropyrolysis procedure was developed for rapidly assessing the relative abundances and variety of different biomarker lipid structures in microbial cultures by reductively converting free functionalised and polymeric lipids within whole cells into hydrocarbons. High pressure hydrogen gas and a molybdenum catalyst were used to target and cleave carbon-oxygen covalent bonds (particularly ester, alcohol. acid and ether) and the pyrolysis process was conducted in an open-system reactor configuration to minimise structural and stereochemical rearrangements in the products. A revised experimental protocol, involving a modified catalyst-loading procedure. careful use of a silica support substrate and a revised temperature program was tested and optimised for handling biomass. Partial hydrogenation of double bonds inevitably did occur although it was found that some unsaturation was preserved, particularly within branched and polycyclic hydrocarbon structures. This experimental approach aids our ability to optimally correlate fossil biomarker signals found in the sedimentary record with their lipid precursors found in extant organisms. Our technique complements more rigorous, but time-consuming, chemical approaches used for elucidating the exact chemical structures of intact functionalised lipids by providing a rapid means by which to screen microbial cultures. (C) 2004 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)63-82
Number of pages19
JournalOrganic Geochemistry
Volume36
Issue number1
DOIs
Publication statusPublished - 2005

Keywords

  • COCCOLITHOPHORID EMILIANIA-HUXLEYI
  • TEMPERATURE-PROGRAMMED REDUCTION
  • ALGA BOTRYOCOCCUS-BRAUNII
  • RESISTANT BIO-POLYMER
  • ORGANIC SULFUR FORMS
  • LONG-CHAIN ALKENES
  • FATTY-ACID
  • L-RACE
  • HALOBACTERIUM-SACCHAROVORUM
  • ALIPHATIC POLYALDEHYDE

Cite this

A catalytic hydropyrolysis method for the rapid screening of microbial cultures for lipid biomarkers. / Love, G. D.; Bowden, Stephen Alan; Jahnke, L. L.; Snape, C. E.; Campbell, C. N.; Day, D.; Summons, R. E.

In: Organic Geochemistry, Vol. 36, No. 1, 2005, p. 63-82.

Research output: Contribution to journalArticle

Love, G. D. ; Bowden, Stephen Alan ; Jahnke, L. L. ; Snape, C. E. ; Campbell, C. N. ; Day, D. ; Summons, R. E. / A catalytic hydropyrolysis method for the rapid screening of microbial cultures for lipid biomarkers. In: Organic Geochemistry. 2005 ; Vol. 36, No. 1. pp. 63-82.
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