A COL1A1 Sp1 binding site polymorphism predisposes to osteoporotic fracture by affecting bone density and quality

Val Mann, Emma Elizabeth Hobson, Tracy Lorraine Stewart, Richard Malcolm Aspden, S Ralston, B. Li, S. F. Grant, S. P. Robins

Research output: Contribution to journalArticle

342 Citations (Scopus)

Abstract

Osteoporosis is a common disease with a strong genetic component. We previously described a polymorphic Spl binding site in the COL1A1 gene that has been associated with osteoporosis in several populations. Here we explore the molecular mechanisms underlying this association. A meta-analysis showed significant associations between COL1A1 "s" alleles and bone mineral density (BMD), body mass index (BMI), and osteoporotic fractures. The association with fracture was stronger than expected on the basis of the observed differences in BMD and BMI, suggesting an additional effect on bone strength. Gel shift assays showed increased binding affinity of the "s" allele for Spl protein, and primary RNA transcripts derived from the "s" allele were approximately three times more abundant than "S" allele-derived transcripts in "Ss" heterozygotes. Collagen produced from osteoblasts cultured from "Ss" heterozygotes had an increased ratio of alpha1(I) protein relative to alpha2(I), and this was accompanied by an increased ratio of COL1A1 mRNA relative to COL1A2. Finally, the yield strength of bone derived from "Ss" individuals was reduced when compared with bone derived from "SS" subjects. We conclude that the COL1A1 Spl polymorphism is a functional genetic variant that predisposes to osteoporosis by complex mechanisms involving changes in bone mass and bone quality.

Original languageEnglish
Pages (from-to)899-907
Number of pages8
JournalThe Journal of Clinical Investigation
Volume107
Issue number7
Publication statusPublished - 2001

Keywords

  • I ALPHA-1 GENE
  • MINERAL DENSITY
  • OSTEOGENESIS IMPERFECTA
  • PREMENOPAUSAL WOMEN
  • POSTMENOPAUSAL WOMEN
  • CANCELLOUS BONE
  • COLLAGEN
  • TURNOVER
  • MICE
  • RISK

Cite this

A COL1A1 Sp1 binding site polymorphism predisposes to osteoporotic fracture by affecting bone density and quality. / Mann, Val; Hobson, Emma Elizabeth; Stewart, Tracy Lorraine; Aspden, Richard Malcolm; Ralston, S; Li, B.; Grant, S. F.; Robins, S. P.

In: The Journal of Clinical Investigation, Vol. 107, No. 7, 2001, p. 899-907.

Research output: Contribution to journalArticle

Mann, V, Hobson, EE, Stewart, TL, Aspden, RM, Ralston, S, Li, B, Grant, SF & Robins, SP 2001, 'A COL1A1 Sp1 binding site polymorphism predisposes to osteoporotic fracture by affecting bone density and quality', The Journal of Clinical Investigation, vol. 107, no. 7, pp. 899-907.
Mann, Val ; Hobson, Emma Elizabeth ; Stewart, Tracy Lorraine ; Aspden, Richard Malcolm ; Ralston, S ; Li, B. ; Grant, S. F. ; Robins, S. P. / A COL1A1 Sp1 binding site polymorphism predisposes to osteoporotic fracture by affecting bone density and quality. In: The Journal of Clinical Investigation. 2001 ; Vol. 107, No. 7. pp. 899-907.
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AU - Mann, Val

AU - Hobson, Emma Elizabeth

AU - Stewart, Tracy Lorraine

AU - Aspden, Richard Malcolm

AU - Ralston, S

AU - Li, B.

AU - Grant, S. F.

AU - Robins, S. P.

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N2 - Osteoporosis is a common disease with a strong genetic component. We previously described a polymorphic Spl binding site in the COL1A1 gene that has been associated with osteoporosis in several populations. Here we explore the molecular mechanisms underlying this association. A meta-analysis showed significant associations between COL1A1 "s" alleles and bone mineral density (BMD), body mass index (BMI), and osteoporotic fractures. The association with fracture was stronger than expected on the basis of the observed differences in BMD and BMI, suggesting an additional effect on bone strength. Gel shift assays showed increased binding affinity of the "s" allele for Spl protein, and primary RNA transcripts derived from the "s" allele were approximately three times more abundant than "S" allele-derived transcripts in "Ss" heterozygotes. Collagen produced from osteoblasts cultured from "Ss" heterozygotes had an increased ratio of alpha1(I) protein relative to alpha2(I), and this was accompanied by an increased ratio of COL1A1 mRNA relative to COL1A2. Finally, the yield strength of bone derived from "Ss" individuals was reduced when compared with bone derived from "SS" subjects. We conclude that the COL1A1 Spl polymorphism is a functional genetic variant that predisposes to osteoporosis by complex mechanisms involving changes in bone mass and bone quality.

AB - Osteoporosis is a common disease with a strong genetic component. We previously described a polymorphic Spl binding site in the COL1A1 gene that has been associated with osteoporosis in several populations. Here we explore the molecular mechanisms underlying this association. A meta-analysis showed significant associations between COL1A1 "s" alleles and bone mineral density (BMD), body mass index (BMI), and osteoporotic fractures. The association with fracture was stronger than expected on the basis of the observed differences in BMD and BMI, suggesting an additional effect on bone strength. Gel shift assays showed increased binding affinity of the "s" allele for Spl protein, and primary RNA transcripts derived from the "s" allele were approximately three times more abundant than "S" allele-derived transcripts in "Ss" heterozygotes. Collagen produced from osteoblasts cultured from "Ss" heterozygotes had an increased ratio of alpha1(I) protein relative to alpha2(I), and this was accompanied by an increased ratio of COL1A1 mRNA relative to COL1A2. Finally, the yield strength of bone derived from "Ss" individuals was reduced when compared with bone derived from "SS" subjects. We conclude that the COL1A1 Spl polymorphism is a functional genetic variant that predisposes to osteoporosis by complex mechanisms involving changes in bone mass and bone quality.

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KW - POSTMENOPAUSAL WOMEN

KW - CANCELLOUS BONE

KW - COLLAGEN

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