A cysteine desulphurase gene from the cellulolytic rumen anaerobe Ruminococcus flavefaciens

J Kirby, F Wright, H J Flint

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Abstract

A gene whose predicted product shows 40-50% sequence identity with the products of nifS genes from nitrogen-fixing bacteria was found downstream from a cellulase gene in a DNA fragment from the cellulolytic rumen anaerobe, Ruminococus flavefaciens 17. The R. flavefaciens gene product released sulphur from L-cysteine when expressed in Escherichia coli, indicating that the R. flavefaciens NifS enzyme may play a role in sulphuration, perhaps, as in nitrogen-fixing bacteria, supplying sulphur to FeS proteins. Sequences hybridising with the R flavefaciens 17 nifS-like gene were also detected in R flavefaciens 007 and in R albus SY3. (C) 1998 Elsevier Science B.V. All rights reserved.

Original languageEnglish
Pages (from-to)233-237
Number of pages5
JournalBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology
Volume1386
Issue number1
Publication statusPublished - 28 Jul 1998

Keywords

  • cysteine desulfurase
  • nifS
  • rumen
  • cellulolysis
  • (Ruminococcus flavefaciens)
  • AZOTOBACTER-VINELANDII
  • ESCHERICHIA-COLI
  • CLOSTRIDIUM-THERMOCELLUM
  • RNASE-H
  • NIFS
  • CLUSTER
  • CLONING
  • NITROGENASES
  • BIOSYNTHESIS
  • CELLULOSOME

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