A diet rich in unsaturated fatty acids prevents progression toward heart failure in a rabbit model of pressure and volume overload

Hester M Den Ruijter, Arie O Verkerk, Cees A Schumacher, Sander M Houten, Charly N W Belterman, Antonius Baartscheer, Ingeborg A Brouwer, Marc van Bilsen, Baukje de Roos, Ruben Coronel

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    17 Citations (Scopus)

    Abstract

    Background—During heart failure (HF), cardiac metabolic substrate preference changes from fatty acid (FA) toward glucose oxidation. This change may cause progression toward heart failure. We hypothesize that a diet rich in FAs may prevent this process, and that dietary ¿3-FAs have an added antiarrhythmic effect based on action potential (AP) shortening in animals with HF.

    Methods and Results—Rabbits were fed a diet containing 1.25% (w/w) high oleic sunflower oil (HF-¿9, N=11), 1.25% fish oil (HF-¿3, N=11), or no supplement (HF-control, N=8). Subsequently, HF was induced by volume and pressure overload. After 4 months, HF-parameters were assessed, electrocardiograms were recorded, and blood and ventricular tissue were collected. Myocytes were isolated for patch clamp or intracellular Ca2+- recordings to study electrophysiologic remodeling and arrhythmogenesis. Both the HF-¿9 and the HF-¿3 groups had larger myocardial FA oxidation capacity than HF control. The HF-¿3 group had significantly lower mean (± SEM) relative heart and lung weight (3.3±0.13 and 3.2±0.12 g kg-1, respectively) than HF control (4.8±0.30 and 4.5±0.23), and shorter QTc intervals (167±2.6 versus 182±6.4). The HF-¿9 also displayed a significantly reduced relative heart weight (3.6±0.26), but had similar QTc (179±4.3) compared with HF control. AP duration in the HF-¿3 group was ˜20% shorter due to increased Ito1 and IK1 and triggered activity, and Ca2+-aftertransients were less than in the HF-¿9 group.

    Conclusions—Dietary unsaturated FAs started prior to induction of HF prevent hypertrophy and HF. In addition, fish oil FAs prevent HF-induced electrophysiologic remodeling and arrhythmias.

    Original languageEnglish
    Pages (from-to)376-384
    Number of pages9
    JournalCirculation. Heart Failure
    Volume5
    Issue number3
    Early online date2 Apr 2012
    DOIs
    Publication statusPublished - 2012

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  • Cite this

    Den Ruijter, H. M., Verkerk, A. O., Schumacher, C. A., Houten, S. M., Belterman, C. N. W., Baartscheer, A., Brouwer, I. A., van Bilsen, M., de Roos, B., & Coronel, R. (2012). A diet rich in unsaturated fatty acids prevents progression toward heart failure in a rabbit model of pressure and volume overload. Circulation. Heart Failure, 5(3), 376-384. https://doi.org/10.1161/CIRCHEARTFAILURE.111.963116