A directed miniscreen for genes involved in the Drosophila anti-parasitoid immune response.

Laura Karen Howell, Christopher John Sampson, Miguel Xavier, E Bolukbasi, MM Heck, Michael Jon Williams

Research output: Contribution to conferencePaper

15 Citations (Scopus)

Abstract

Drosophila larvae react against eggs from the endoparasitoid wasp Leptopilina boulardi by surrounding them in a multilayered cellular capsule. Once a wasp egg is recognized as foreign, circulating macrophage-like cells, known as plasmatocytes, adhere to the invader. After spreading around the wasp egg, plasmatocytes form cellular junctions between the cells, effectively separating the egg from the hemocoel. Next, a second sub-type of circulating immunosurveillance cell (hemocyte), known as lamellocytes, adhere to either the wasp egg or more likely the plasmatocytes surrounding the egg. From these events, it is obvious that adhesion and cell shape change are an essential part of Drosophila's cellular immune response against parasitoid wasp eggs. To date, very few genes have been described as being necessary for a proper anti-parasitization response in Drosophila. With this in mind, we performed a directed genetic miniscreen to discover new genes required for this response. Many of the genes with an encapsulation defect have mammalian homologues involved in cellular adhesion, wound healing, and thrombosis, including extracellular matrix proteins, cellular adhesion molecules, and small GTPases.
Original languageEnglish
Pages155-161
Number of pages7
DOIs
Publication statusPublished - Feb 2012

Fingerprint

Drosophila
immune response
cell adhesion
Leptopilina boulardi
genes
cells
hemocoel
thrombosis
guanosinetriphosphatase
encapsulation
tissue repair
hemocytes
extracellular matrix
cell-mediated immunity
adhesion
parasitism
macrophages
larvae
proteins

Keywords

  • DROSOPHILA
  • Immune response
  • parasitoid wasp (Aphidius ervi)

Cite this

Howell, L. K., Sampson, C. J., Xavier, M., Bolukbasi, E., Heck, MM., & Williams, M. J. (2012). A directed miniscreen for genes involved in the Drosophila anti-parasitoid immune response.. 155-161. https://doi.org/10.1007/s00251-011-0571-3

A directed miniscreen for genes involved in the Drosophila anti-parasitoid immune response. / Howell, Laura Karen; Sampson, Christopher John; Xavier, Miguel; Bolukbasi, E; Heck, MM; Williams, Michael Jon.

2012. 155-161.

Research output: Contribution to conferencePaper

Howell, LK, Sampson, CJ, Xavier, M, Bolukbasi, E, Heck, MM & Williams, MJ 2012, 'A directed miniscreen for genes involved in the Drosophila anti-parasitoid immune response.' pp. 155-161. https://doi.org/10.1007/s00251-011-0571-3
Howell, Laura Karen ; Sampson, Christopher John ; Xavier, Miguel ; Bolukbasi, E ; Heck, MM ; Williams, Michael Jon. / A directed miniscreen for genes involved in the Drosophila anti-parasitoid immune response. 7 p.
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abstract = "Drosophila larvae react against eggs from the endoparasitoid wasp Leptopilina boulardi by surrounding them in a multilayered cellular capsule. Once a wasp egg is recognized as foreign, circulating macrophage-like cells, known as plasmatocytes, adhere to the invader. After spreading around the wasp egg, plasmatocytes form cellular junctions between the cells, effectively separating the egg from the hemocoel. Next, a second sub-type of circulating immunosurveillance cell (hemocyte), known as lamellocytes, adhere to either the wasp egg or more likely the plasmatocytes surrounding the egg. From these events, it is obvious that adhesion and cell shape change are an essential part of Drosophila's cellular immune response against parasitoid wasp eggs. To date, very few genes have been described as being necessary for a proper anti-parasitization response in Drosophila. With this in mind, we performed a directed genetic miniscreen to discover new genes required for this response. Many of the genes with an encapsulation defect have mammalian homologues involved in cellular adhesion, wound healing, and thrombosis, including extracellular matrix proteins, cellular adhesion molecules, and small GTPases.",
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