This study demonstrates a novel method to quantify selective (1,4-NADH) and unselective products (1,2- and 1,6-NADH) in NADH regeneration using combined UV-Vis spectroscopy and biological assays. The validity of the proposed method was tested in the Pt/C promoted NAD+ hydrogenation using hydrogen as reducing agent.
Saba, T., Burnett, J. W. H., Li, J., Kechagiopoulos, P., & Wang, X. (2020). A facile analytical method for reliable selectivity examination in cofactor NADH regeneration. Chemical Communications, 56(8), 1231-1234. https://doi.org/10.1039/c9cc07805c