A fluorescent coagulation assay for thrombin using a fibre optic evanescent wave sensor

S. R. Garden, G. J. Doellgast, Kenneth Stuart Killham, Norval James Colin Strachan

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15 Citations (Scopus)

Abstract

A fibre optic evanescent wave sensor is used for the rapid detection of thrombin. Coagulation of solution phase fluorescently labelled fibrinogen to unlabelled fibrinogen bound to the surface of the fibre optic is observed in real time by the evanescent wave sensor. Thrombin concentrations down to 0.01 NIH ml(-1) are detectable within 5 min. The potential application of this technique for rapid amplified immunosensing is discussed. (C) 2003 Elsevier B.V. All rights reserved.

Original languageEnglish
Pages (from-to)737-740
Number of pages3
JournalBiosensors & Bioelectronics
Volume19
DOIs
Publication statusPublished - 2004

Keywords

  • fibre optic sensor
  • evanescent wave sensor
  • thrombin
  • blood coagulation
  • fluorescent fibrinogen
  • SURFACE-PLASMON RESONANCE
  • WHOLE-BLOOD
  • BIOSENSOR

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