A fluorescent coagulation assay for thrombin using a fibre optic evanescent wave sensor

S. R. Garden; G. J. Doellgast; Kenneth Stuart Killham; Norval James Colin Strachan

doi:10.1016/j.bios.2003.08.002

A fluorescent coagulation assay for thrombin using a fibre optic evanescent wave sensor

S. R. Garden, G. J. Doellgast, Kenneth Stuart Killham, Norval James Colin Strachan

Physics

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

A fibre optic evanescent wave sensor is used for the rapid detection of thrombin. Coagulation of solution phase fluorescently labelled fibrinogen to unlabelled fibrinogen bound to the surface of the fibre optic is observed in real time by the evanescent wave sensor. Thrombin concentrations down to 0.01 NIH ml(-1) are detectable within 5 min. The potential application of this technique for rapid amplified immunosensing is discussed. (C) 2003 Elsevier B.V. All rights reserved.

Original language	English
Pages (from-to)	737-740
Number of pages	3
Journal	Biosensors & Bioelectronics
Volume	19
DOIs	https://doi.org/10.1016/j.bios.2003.08.002 https://doi.org/10.1016/j.bios.2004.04.012
Publication status	Published - 2004

Keywords

fibre optic sensor
evanescent wave sensor
thrombin
blood coagulation
fluorescent fibrinogen
SURFACE-PLASMON RESONANCE
WHOLE-BLOOD
BIOSENSOR

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title = "A fluorescent coagulation assay for thrombin using a fibre optic evanescent wave sensor",

abstract = "A fibre optic evanescent wave sensor is used for the rapid detection of thrombin. Coagulation of solution phase fluorescently labelled fibrinogen to unlabelled fibrinogen bound to the surface of the fibre optic is observed in real time by the evanescent wave sensor. Thrombin concentrations down to 0.01 NIH ml(-1) are detectable within 5 min. The potential application of this technique for rapid amplified immunosensing is discussed. (C) 2003 Elsevier B.V. All rights reserved.",

keywords = "fibre optic sensor, evanescent wave sensor, thrombin, blood coagulation, fluorescent fibrinogen, SURFACE-PLASMON RESONANCE, WHOLE-BLOOD, BIOSENSOR",

author = "Garden, {S. R.} and Doellgast, {G. J.} and Killham, {Kenneth Stuart} and Strachan, {Norval James Colin}",

note = "Erratum: A fluorescent coagulation assay for thrombin using a fibre optic evanescent wave sensor (Biosensors and Bioelectronics (2004) 19 (1775) DOI: 10.1016/j.bios.2003.08.002)",

year = "2004",

doi = "10.1016/j.bios.2003.08.002",

language = "English",

volume = "19",

pages = "737--740",

journal = "Biosensors & Bioelectronics",

issn = "0956-5663",

publisher = "Elsevier Limited",

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TY - JOUR

T1 - A fluorescent coagulation assay for thrombin using a fibre optic evanescent wave sensor

AU - Garden, S. R.

AU - Doellgast, G. J.

AU - Killham, Kenneth Stuart

AU - Strachan, Norval James Colin

N1 - Erratum: A fluorescent coagulation assay for thrombin using a fibre optic evanescent wave sensor (Biosensors and Bioelectronics (2004) 19 (1775) DOI: 10.1016/j.bios.2003.08.002)

PY - 2004

Y1 - 2004

N2 - A fibre optic evanescent wave sensor is used for the rapid detection of thrombin. Coagulation of solution phase fluorescently labelled fibrinogen to unlabelled fibrinogen bound to the surface of the fibre optic is observed in real time by the evanescent wave sensor. Thrombin concentrations down to 0.01 NIH ml(-1) are detectable within 5 min. The potential application of this technique for rapid amplified immunosensing is discussed. (C) 2003 Elsevier B.V. All rights reserved.

AB - A fibre optic evanescent wave sensor is used for the rapid detection of thrombin. Coagulation of solution phase fluorescently labelled fibrinogen to unlabelled fibrinogen bound to the surface of the fibre optic is observed in real time by the evanescent wave sensor. Thrombin concentrations down to 0.01 NIH ml(-1) are detectable within 5 min. The potential application of this technique for rapid amplified immunosensing is discussed. (C) 2003 Elsevier B.V. All rights reserved.

KW - fibre optic sensor

KW - evanescent wave sensor

KW - thrombin

KW - blood coagulation

KW - fluorescent fibrinogen

KW - SURFACE-PLASMON RESONANCE

KW - WHOLE-BLOOD

KW - BIOSENSOR

U2 - 10.1016/j.bios.2003.08.002

DO - 10.1016/j.bios.2003.08.002

M3 - Article

VL - 19

SP - 737

EP - 740

JO - Biosensors & Bioelectronics

JF - Biosensors & Bioelectronics

SN - 0956-5663

ER -

A fluorescent coagulation assay for thrombin using a fibre optic evanescent wave sensor

Abstract

Keywords

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