@inbook{c61aecc8ec2a45cf8eca6f6eec122534,
title = "A Functional Assay for GPR55: Envision Protocol",
abstract = "AlphaScreen{\textregistered} SureFire{\textregistered} assay is a novel technology that combines luminescent oxygen channeling technology, nano-beads, and monocloncal antibodies to detect the level of a selected protein in a volume lower than 5 μl. This method is more sensitive compared with the traditional enzyme-linked immunosorbent assays (ELISA), and can detect an increasing number of new targets. Here, we described a method for AlphaScreen{\textregistered} SureFire{\textregistered} assay that targets ERK1/2 phosphorylation, a primary downstream signaling pathway that conveys activation of GPR55 by l-α-lysophosphatidylinositol (LPI) and certain cannabinoids.",
keywords = "AlphaScreen, Enspire, AlphaLISA, GPR55, Phytocannabinoids, High-throughput screening, Cannabinoid receptor",
author = "Sharon Anavi-Goffer and Ross, {Ruth A.}",
note = "Acknowledgments This work was supported by the US National Institutes of Health grant numbers DA-03672 and DA-09789 (RAR). We thank the University of Aberdeen for a Knowledge Transfer Grant Award.",
year = "2016",
doi = "10.1007/978-1-4939-3539-0_8",
language = "English",
isbn = "978-1-4939-3537-6",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "77--83",
editor = "Mauro Maccarrone",
booktitle = "Endocannabinoid Signaling",
address = "United States",
}