A Functional Assay for GPR55: Envision Protocol

Sharon Anavi-Goffer; Ruth A. Ross

doi:10.1007/978-1-4939-3539-0_8

A Functional Assay for GPR55: Envision Protocol

Sharon Anavi-Goffer^*, Ruth A. Ross

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Chapter

2 Citations (Scopus)

Abstract

AlphaScreen® SureFire® assay is a novel technology that combines luminescent oxygen channeling technology, nano-beads, and monocloncal antibodies to detect the level of a selected protein in a volume lower than 5 μl. This method is more sensitive compared with the traditional enzyme-linked immunosorbent assays (ELISA), and can detect an increasing number of new targets. Here, we described a method for AlphaScreen® SureFire® assay that targets ERK1/2 phosphorylation, a primary downstream signaling pathway that conveys activation of GPR55 by l-α-lysophosphatidylinositol (LPI) and certain cannabinoids.

Original language	English
Title of host publication	Endocannabinoid Signaling
Subtitle of host publication	Methods and Protocols
Editors	Mauro Maccarrone
Place of Publication	New York
Publisher	Humana Press
Pages	77-83
Number of pages	7
ISBN (Electronic)	978-1-4939-3539-0
ISBN (Print)	978-1-4939-3537-6
DOIs	https://doi.org/10.1007/978-1-4939-3539-0_8
Publication status	Published - 2016

Publication series

Name	Methods in Molecular Biology
Publisher	Springer
Volume	1412
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Bibliographical note

Acknowledgments
This work was supported by the US National Institutes of Health grant numbers DA-03672 and DA-09789 (RAR). We thank the University of Aberdeen for a Knowledge Transfer Grant Award.

Keywords

AlphaScreen
Enspire
AlphaLISA
GPR55
Phytocannabinoids
High-throughput screening
Cannabinoid receptor

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Cite this

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title = "A Functional Assay for GPR55: Envision Protocol",

abstract = "AlphaScreen{\textregistered} SureFire{\textregistered} assay is a novel technology that combines luminescent oxygen channeling technology, nano-beads, and monocloncal antibodies to detect the level of a selected protein in a volume lower than 5 μl. This method is more sensitive compared with the traditional enzyme-linked immunosorbent assays (ELISA), and can detect an increasing number of new targets. Here, we described a method for AlphaScreen{\textregistered} SureFire{\textregistered} assay that targets ERK1/2 phosphorylation, a primary downstream signaling pathway that conveys activation of GPR55 by l-α-lysophosphatidylinositol (LPI) and certain cannabinoids.",

keywords = "AlphaScreen, Enspire, AlphaLISA, GPR55, Phytocannabinoids, High-throughput screening, Cannabinoid receptor",

author = "Sharon Anavi-Goffer and Ross, {Ruth A.}",

note = "Acknowledgments This work was supported by the US National Institutes of Health grant numbers DA-03672 and DA-09789 (RAR). We thank the University of Aberdeen for a Knowledge Transfer Grant Award.",

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doi = "10.1007/978-1-4939-3539-0_8",

language = "English",

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AB - AlphaScreen® SureFire® assay is a novel technology that combines luminescent oxygen channeling technology, nano-beads, and monocloncal antibodies to detect the level of a selected protein in a volume lower than 5 μl. This method is more sensitive compared with the traditional enzyme-linked immunosorbent assays (ELISA), and can detect an increasing number of new targets. Here, we described a method for AlphaScreen® SureFire® assay that targets ERK1/2 phosphorylation, a primary downstream signaling pathway that conveys activation of GPR55 by l-α-lysophosphatidylinositol (LPI) and certain cannabinoids.

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KW - AlphaLISA

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KW - Phytocannabinoids

KW - High-throughput screening

KW - Cannabinoid receptor

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A Functional Assay for GPR55: Envision Protocol

Abstract

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Bibliographical note

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