A Functional Assay for GPR55: Envision Protocol

Sharon Anavi-Goffer*, Ruth A. Ross

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)


AlphaScreen® SureFire® assay is a novel technology that combines luminescent oxygen channeling technology, nano-beads, and monocloncal antibodies to detect the level of a selected protein in a volume lower than 5 μl. This method is more sensitive compared with the traditional enzyme-linked immunosorbent assays (ELISA), and can detect an increasing number of new targets. Here, we described a method for AlphaScreen® SureFire® assay that targets ERK1/2 phosphorylation, a primary downstream signaling pathway that conveys activation of GPR55 by l-α-lysophosphatidylinositol (LPI) and certain cannabinoids.
Original languageEnglish
Title of host publicationEndocannabinoid Signaling
Subtitle of host publicationMethods and Protocols
EditorsMauro Maccarrone
Place of PublicationNew York
PublisherHumana Press
Number of pages7
ISBN (Electronic)978-1-4939-3539-0
ISBN (Print)978-1-4939-3537-6
Publication statusPublished - 2016

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • AlphaScreen
  • Enspire
  • AlphaLISA
  • GPR55
  • Phytocannabinoids
  • High-throughput screening
  • Cannabinoid receptor


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