A Functional Assay for GPR55: Envision Protocol

Sharon Anavi-Goffer*, Ruth A. Ross

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

AlphaScreen® SureFire® assay is a novel technology that combines luminescent oxygen channeling technology, nano-beads, and monocloncal antibodies to detect the level of a selected protein in a volume lower than 5 μl. This method is more sensitive compared with the traditional enzyme-linked immunosorbent assays (ELISA), and can detect an increasing number of new targets. Here, we described a method for AlphaScreen® SureFire® assay that targets ERK1/2 phosphorylation, a primary downstream signaling pathway that conveys activation of GPR55 by l-α-lysophosphatidylinositol (LPI) and certain cannabinoids.
Original languageEnglish
Title of host publicationEndocannabinoid Signaling
Subtitle of host publicationMethods and Protocols
EditorsMauro Maccarrone
Place of PublicationNew York
PublisherHumana Press
Pages77-83
Number of pages7
ISBN (Electronic)978-1-4939-3539-0
ISBN (Print)978-1-4939-3537-6
DOIs
Publication statusPublished - 2016

Publication series

NameMethods in Molecular Biology
PublisherSpringer
Volume1412
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • AlphaScreen
  • Enspire
  • AlphaLISA
  • GPR55
  • Phytocannabinoids
  • High-throughput screening
  • Cannabinoid receptor

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  • Cite this

    Anavi-Goffer, S., & Ross, R. A. (2016). A Functional Assay for GPR55: Envision Protocol. In M. Maccarrone (Ed.), Endocannabinoid Signaling: Methods and Protocols (pp. 77-83). (Methods in Molecular Biology; Vol. 1412). Humana Press. https://doi.org/10.1007/978-1-4939-3539-0_8