Abstract
Human growth hormone is frequently used as a reporter gene in studies addressing the regulation of eukaryotic promoters. Here we present evidence that the first intron of the hGH-N coding region contains a novel eukaryotic promoter which is able to direct the expression of hGH-N and luciferase reporter genes. The corresponding transcriptional initiation site is located downstream of the splice acceptor site of exon 2. This internal hGH-N promoter can interfere with the transcriptional control elements of a promoter linked to the hGH-N coding region and thereby complicate the interpretation of data obtained with hGH-N as a reporter gene. (C) 1998 Academic Press.
| Original language | English |
|---|---|
| Pages (from-to) | 332-337 |
| Number of pages | 6 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 247 |
| Issue number | 2 |
| Publication status | Published - 18 Jun 1998 |
Keywords
- growth-hormone gene
- transgenic mice
- glucocorticoid receptor
- mammary-gland
- 1ST intron
- expression
- transcription
- sequences
- element
- cells