A functional eukaryotic promoter is contained within the first intron of the hGH-N coding region

Andreas Kolb, W H Gunzburg, G Brem, V Erfle, B Salmons

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Human growth hormone is frequently used as a reporter gene in studies addressing the regulation of eukaryotic promoters. Here we present evidence that the first intron of the hGH-N coding region contains a novel eukaryotic promoter which is able to direct the expression of hGH-N and luciferase reporter genes. The corresponding transcriptional initiation site is located downstream of the splice acceptor site of exon 2. This internal hGH-N promoter can interfere with the transcriptional control elements of a promoter linked to the hGH-N coding region and thereby complicate the interpretation of data obtained with hGH-N as a reporter gene. (C) 1998 Academic Press.

Original languageEnglish
Pages (from-to)332-337
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume247
Issue number2
Publication statusPublished - 18 Jun 1998

Keywords

  • growth-hormone gene
  • transgenic mice
  • glucocorticoid receptor
  • mammary-gland
  • 1ST intron
  • expression
  • transcription
  • sequences
  • element
  • cells

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