A functional eukaryotic promoter is contained within the first intron of the hGH-N coding region

Andreas Kolb, W H Gunzburg, G Brem, V Erfle, B Salmons

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    11 Citations (Scopus)

    Abstract

    Human growth hormone is frequently used as a reporter gene in studies addressing the regulation of eukaryotic promoters. Here we present evidence that the first intron of the hGH-N coding region contains a novel eukaryotic promoter which is able to direct the expression of hGH-N and luciferase reporter genes. The corresponding transcriptional initiation site is located downstream of the splice acceptor site of exon 2. This internal hGH-N promoter can interfere with the transcriptional control elements of a promoter linked to the hGH-N coding region and thereby complicate the interpretation of data obtained with hGH-N as a reporter gene. (C) 1998 Academic Press.

    Original languageEnglish
    Pages (from-to)332-337
    Number of pages6
    JournalBiochemical and Biophysical Research Communications
    Volume247
    Issue number2
    Publication statusPublished - 18 Jun 1998

    Keywords

    • growth-hormone gene
    • transgenic mice
    • glucocorticoid receptor
    • mammary-gland
    • 1ST intron
    • expression
    • transcription
    • sequences
    • element
    • cells

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