A functional eukaryotic promoter is contained within the first intron of the hGH-N coding region

Andreas Kolb, W H Gunzburg, G Brem, V Erfle, B Salmons

    Research output: Contribution to journalArticle

    11 Citations (Scopus)

    Abstract

    Human growth hormone is frequently used as a reporter gene in studies addressing the regulation of eukaryotic promoters. Here we present evidence that the first intron of the hGH-N coding region contains a novel eukaryotic promoter which is able to direct the expression of hGH-N and luciferase reporter genes. The corresponding transcriptional initiation site is located downstream of the splice acceptor site of exon 2. This internal hGH-N promoter can interfere with the transcriptional control elements of a promoter linked to the hGH-N coding region and thereby complicate the interpretation of data obtained with hGH-N as a reporter gene. (C) 1998 Academic Press.

    Original languageEnglish
    Pages (from-to)332-337
    Number of pages6
    JournalBiochemical and Biophysical Research Communications
    Volume247
    Issue number2
    Publication statusPublished - 18 Jun 1998

    Keywords

    • growth-hormone gene
    • transgenic mice
    • glucocorticoid receptor
    • mammary-gland
    • 1ST intron
    • expression
    • transcription
    • sequences
    • element
    • cells

    Cite this

    A functional eukaryotic promoter is contained within the first intron of the hGH-N coding region. / Kolb, Andreas; Gunzburg, W H ; Brem, G ; Erfle, V ; Salmons, B .

    In: Biochemical and Biophysical Research Communications, Vol. 247, No. 2, 18.06.1998, p. 332-337.

    Research output: Contribution to journalArticle

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    abstract = "Human growth hormone is frequently used as a reporter gene in studies addressing the regulation of eukaryotic promoters. Here we present evidence that the first intron of the hGH-N coding region contains a novel eukaryotic promoter which is able to direct the expression of hGH-N and luciferase reporter genes. The corresponding transcriptional initiation site is located downstream of the splice acceptor site of exon 2. This internal hGH-N promoter can interfere with the transcriptional control elements of a promoter linked to the hGH-N coding region and thereby complicate the interpretation of data obtained with hGH-N as a reporter gene. (C) 1998 Academic Press.",
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    AU - Kolb, Andreas

    AU - Gunzburg, W H

    AU - Brem, G

    AU - Erfle, V

    AU - Salmons, B

    PY - 1998/6/18

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    KW - transgenic mice

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    KW - mammary-gland

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    KW - sequences

    KW - element

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