A genetic interval and physical contig spanning the Peronospora parasitica (At) avirulence gene locus ATR1Nd

Anne P Rehmany; Laura Joy Grenville; Nick D Gunn; Rebecca L. Allen; Zennia Paniwnyk; Jane Byrne; Stephen C Whisson; Paul R. J. Birch; Jim L Beynon

doi:10.1016/S1087-1845(02)00515-7

A genetic interval and physical contig spanning the Peronospora parasitica (At) avirulence gene locus ATR1Nd

Anne P Rehmany, Laura Joy Grenville, Nick D Gunn, Rebecca L. Allen, Zennia Paniwnyk, Jane Byrne, Stephen C Whisson, Paul R. J. Birch, Jim L Beynon

Medical Sciences

Research output: Contribution to journal › Article

22 Citations (Scopus)

Abstract

In Peronospora parasitica (At) (downy mildew), the genetic determinants of cultivar-specific recognition by Arabidopsis thaliana are the Arabidopsis thaliana-recognised (ATR) avirulence genes. We describe the identification of 10 amplified fragment length polymorphism (AFLP) markers that define a genetic mapping interval for the ATR1Nd avirulence allele, the presence of which is perceived by the RPP1Nd resistance gene. Furthermore, we have constructed a P. parasitica (At) bacterial artificial chromosome (BAC) library comprising over 630Mb of cloned DNA. We have isolated 16 overlapping clones from the BAC library that form a contig spanning the genetic interval. BAC sequence-derived markers and a total mapping population of 311 F(2) individuals were used to refine the ATR1Nd locus to a 1cM interval that is represented by four BAC clones and spans less than 250kb of DNA. This work demonstrates that map-based cloning techniques are feasible in this organism and provides the critical foundations for cloning ATR1Nd using such a strategy.

Original language	English
Pages (from-to)	33-42
Number of pages	10
Journal	Fungal Genetics and Biology
Volume	38
Issue number	1
Early online date	30 Oct 2002
DOIs	https://doi.org/10.1016/S1087-1845(02)00515-7
Publication status	Published - 1 Feb 2003

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Keywords

Alleles
Arabidopsis
Chromosomes, Artificial, Bacterial
Cloning, Molecular
Contig Mapping
DNA
Genetic Markers
Genomic Library
Oomycetes
Physical Chromosome Mapping
Phytophthora

Cite this

Rehmany, A. P., Grenville, L. J., Gunn, N. D., Allen, R. L., Paniwnyk, Z., Byrne, J., ... Beynon, J. L. (2003). A genetic interval and physical contig spanning the Peronospora parasitica (At) avirulence gene locus ATR1Nd. Fungal Genetics and Biology, 38(1), 33-42. https://doi.org/10.1016/S1087-1845(02)00515-7

A genetic interval and physical contig spanning the Peronospora parasitica (At) avirulence gene locus ATR1Nd. / Rehmany, Anne P; Grenville, Laura Joy; Gunn, Nick D; Allen, Rebecca L.; Paniwnyk, Zennia; Byrne, Jane; Whisson, Stephen C; Birch, Paul R. J.; Beynon, Jim L.

In: Fungal Genetics and Biology, Vol. 38, No. 1, 01.02.2003, p. 33-42.

Research output: Contribution to journal › Article

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abstract = "In Peronospora parasitica (At) (downy mildew), the genetic determinants of cultivar-specific recognition by Arabidopsis thaliana are the Arabidopsis thaliana-recognised (ATR) avirulence genes. We describe the identification of 10 amplified fragment length polymorphism (AFLP) markers that define a genetic mapping interval for the ATR1Nd avirulence allele, the presence of which is perceived by the RPP1Nd resistance gene. Furthermore, we have constructed a P. parasitica (At) bacterial artificial chromosome (BAC) library comprising over 630Mb of cloned DNA. We have isolated 16 overlapping clones from the BAC library that form a contig spanning the genetic interval. BAC sequence-derived markers and a total mapping population of 311 F(2) individuals were used to refine the ATR1Nd locus to a 1cM interval that is represented by four BAC clones and spans less than 250kb of DNA. This work demonstrates that map-based cloning techniques are feasible in this organism and provides the critical foundations for cloning ATR1Nd using such a strategy.",

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10.1016/S1087-1845(02)00515-7