A genetic interval and physical contig spanning the Peronospora parasitica (At) avirulence gene locus ATR1Nd

Anne P Rehmany, Laura Joy Grenville, Nick D Gunn, Rebecca L. Allen, Zennia Paniwnyk, Jane Byrne, Stephen C Whisson, Paul R. J. Birch, Jim L Beynon

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Abstract

In Peronospora parasitica (At) (downy mildew), the genetic determinants of cultivar-specific recognition by Arabidopsis thaliana are the Arabidopsis thaliana-recognised (ATR) avirulence genes. We describe the identification of 10 amplified fragment length polymorphism (AFLP) markers that define a genetic mapping interval for the ATR1Nd avirulence allele, the presence of which is perceived by the RPP1Nd resistance gene. Furthermore, we have constructed a P. parasitica (At) bacterial artificial chromosome (BAC) library comprising over 630Mb of cloned DNA. We have isolated 16 overlapping clones from the BAC library that form a contig spanning the genetic interval. BAC sequence-derived markers and a total mapping population of 311 F(2) individuals were used to refine the ATR1Nd locus to a 1cM interval that is represented by four BAC clones and spans less than 250kb of DNA. This work demonstrates that map-based cloning techniques are feasible in this organism and provides the critical foundations for cloning ATR1Nd using such a strategy.
Original languageEnglish
Pages (from-to)33-42
Number of pages10
JournalFungal Genetics and Biology
Volume38
Issue number1
Early online date30 Oct 2002
DOIs
Publication statusPublished - 1 Feb 2003

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Keywords

  • Alleles
  • Arabidopsis
  • Chromosomes, Artificial, Bacterial
  • Cloning, Molecular
  • Contig Mapping
  • DNA
  • Genetic Markers
  • Genomic Library
  • Oomycetes
  • Physical Chromosome Mapping
  • Phytophthora

Cite this

Rehmany, A. P., Grenville, L. J., Gunn, N. D., Allen, R. L., Paniwnyk, Z., Byrne, J., ... Beynon, J. L. (2003). A genetic interval and physical contig spanning the Peronospora parasitica (At) avirulence gene locus ATR1Nd. Fungal Genetics and Biology, 38(1), 33-42. https://doi.org/10.1016/S1087-1845(02)00515-7