Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 33-42 |
| Number of pages | 10 |
| Journal | Fungal Genetics and Biology |
| Volume | 38 |
| Issue number | 1 |
| Early online date | 30 Oct 2002 |
| DOIs | |
| Publication status | Published - 1 Feb 2003 |
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Keywords
- Alleles
- Arabidopsis
- Chromosomes, Artificial, Bacterial
- Cloning, Molecular
- Contig Mapping
- DNA
- Genetic Markers
- Genomic Library
- Oomycetes
- Physical Chromosome Mapping
- Phytophthora
Cite this
A genetic interval and physical contig spanning the Peronospora parasitica (At) avirulence gene locus ATR1Nd. / Rehmany, Anne P; Grenville, Laura Joy; Gunn, Nick D; Allen, Rebecca L.; Paniwnyk, Zennia; Byrne, Jane; Whisson, Stephen C; Birch, Paul R. J.; Beynon, Jim L.
In: Fungal Genetics and Biology, Vol. 38, No. 1, 01.02.2003, p. 33-42.Research output: Contribution to journal › Article
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TY - JOUR
T1 - A genetic interval and physical contig spanning the Peronospora parasitica (At) avirulence gene locus ATR1Nd
AU - Rehmany, Anne P
AU - Grenville, Laura Joy
AU - Gunn, Nick D
AU - Allen, Rebecca L.
AU - Paniwnyk, Zennia
AU - Byrne, Jane
AU - Whisson, Stephen C
AU - Birch, Paul R. J.
AU - Beynon, Jim L
PY - 2003/2/1
Y1 - 2003/2/1
N2 - In Peronospora parasitica (At) (downy mildew), the genetic determinants of cultivar-specific recognition by Arabidopsis thaliana are the Arabidopsis thaliana-recognised (ATR) avirulence genes. We describe the identification of 10 amplified fragment length polymorphism (AFLP) markers that define a genetic mapping interval for the ATR1Nd avirulence allele, the presence of which is perceived by the RPP1Nd resistance gene. Furthermore, we have constructed a P. parasitica (At) bacterial artificial chromosome (BAC) library comprising over 630Mb of cloned DNA. We have isolated 16 overlapping clones from the BAC library that form a contig spanning the genetic interval. BAC sequence-derived markers and a total mapping population of 311 F(2) individuals were used to refine the ATR1Nd locus to a 1cM interval that is represented by four BAC clones and spans less than 250kb of DNA. This work demonstrates that map-based cloning techniques are feasible in this organism and provides the critical foundations for cloning ATR1Nd using such a strategy.
AB - In Peronospora parasitica (At) (downy mildew), the genetic determinants of cultivar-specific recognition by Arabidopsis thaliana are the Arabidopsis thaliana-recognised (ATR) avirulence genes. We describe the identification of 10 amplified fragment length polymorphism (AFLP) markers that define a genetic mapping interval for the ATR1Nd avirulence allele, the presence of which is perceived by the RPP1Nd resistance gene. Furthermore, we have constructed a P. parasitica (At) bacterial artificial chromosome (BAC) library comprising over 630Mb of cloned DNA. We have isolated 16 overlapping clones from the BAC library that form a contig spanning the genetic interval. BAC sequence-derived markers and a total mapping population of 311 F(2) individuals were used to refine the ATR1Nd locus to a 1cM interval that is represented by four BAC clones and spans less than 250kb of DNA. This work demonstrates that map-based cloning techniques are feasible in this organism and provides the critical foundations for cloning ATR1Nd using such a strategy.
KW - Alleles
KW - Arabidopsis
KW - Chromosomes, Artificial, Bacterial
KW - Cloning, Molecular
KW - Contig Mapping
KW - DNA
KW - Genetic Markers
KW - Genomic Library
KW - Oomycetes
KW - Physical Chromosome Mapping
KW - Phytophthora
U2 - 10.1016/S1087-1845(02)00515-7
DO - 10.1016/S1087-1845(02)00515-7
M3 - Article
VL - 38
SP - 33
EP - 42
JO - Fungal Genetics and Biology
JF - Fungal Genetics and Biology
SN - 1087-1845
IS - 1
ER -