A Ga subunit controls zoospore motility and virulence in the potato late blight pathogen Phytophthora infestans

M Latijnhouwers, W Ligterink, VG Vleeshouwers, West P van, F Govers

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

The heterotrimeric G-protein pathway is a ubiquitous eukaryotic signalling module that is known to regulate growth and differentiation in many plant pathogens. We previously identified Pigpa1, a gene encoding a G-protein alpha subunit from the potato late blight pathogen Phytophthora infestans. P. infestans belongs to the class oomycetes, a group of organisms in which signal transduction processes have not yet been studied at the molecular level. To elucidate the function of Pigpa1, PiGPA1-deficient mutants were obtained by homology-dependent gene silencing. The Pigpa1-silenced mutants produced zoospores that turned six to eight times more frequently, causing them to swim only short distances compared with wild type. Attraction to the surface, a phenomenon known as negative geotaxis, was impaired in the mutant zoospores, as well as autoaggregation and chemotaxis towards glutamic and aspartic acid. Zoospore production was reduced by 20-45% in different Pigpa1-silenced mutants. Transformants expressing constitutively active forms of PiGPA1, containing amino acid substitutions (R177H and Q203L), showed no obvious phenotypic differences from the wild-type strain. Infection efficiencies on potato leaves ranged from 3% to 14% in the Pigpa1-silenced mutants, compared with 77% in wild type, showing that virulence is severely impaired. The results prove that PiGPA1 is crucial for zoospore motility and for pathogenicity in an important oomycete plant pathogen.

Original languageEnglish
Pages (from-to)925-936
Number of pages12
JournalMolecular Microbiology
Volume51
Issue number4
Early online date22 Dec 2003
DOIs
Publication statusPublished - Feb 2004

Keywords

  • heterotrimeric G-proteins
  • beta-subunit
  • arabidopsis-thaliana
  • fungus phytophthora
  • cryphonectria-parasitica
  • signal-transduction
  • cell-proliferation
  • neurospora-crassa
  • gamma-subunit
  • genes

Cite this

A Ga subunit controls zoospore motility and virulence in the potato late blight pathogen Phytophthora infestans. / Latijnhouwers, M; Ligterink, W; Vleeshouwers, VG; van, West P; Govers, F.

In: Molecular Microbiology, Vol. 51, No. 4, 02.2004, p. 925-936.

Research output: Contribution to journalArticle

Latijnhouwers, M ; Ligterink, W ; Vleeshouwers, VG ; van, West P ; Govers, F. / A Ga subunit controls zoospore motility and virulence in the potato late blight pathogen Phytophthora infestans. In: Molecular Microbiology. 2004 ; Vol. 51, No. 4. pp. 925-936.
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abstract = "The heterotrimeric G-protein pathway is a ubiquitous eukaryotic signalling module that is known to regulate growth and differentiation in many plant pathogens. We previously identified Pigpa1, a gene encoding a G-protein alpha subunit from the potato late blight pathogen Phytophthora infestans. P. infestans belongs to the class oomycetes, a group of organisms in which signal transduction processes have not yet been studied at the molecular level. To elucidate the function of Pigpa1, PiGPA1-deficient mutants were obtained by homology-dependent gene silencing. The Pigpa1-silenced mutants produced zoospores that turned six to eight times more frequently, causing them to swim only short distances compared with wild type. Attraction to the surface, a phenomenon known as negative geotaxis, was impaired in the mutant zoospores, as well as autoaggregation and chemotaxis towards glutamic and aspartic acid. Zoospore production was reduced by 20-45{\%} in different Pigpa1-silenced mutants. Transformants expressing constitutively active forms of PiGPA1, containing amino acid substitutions (R177H and Q203L), showed no obvious phenotypic differences from the wild-type strain. Infection efficiencies on potato leaves ranged from 3{\%} to 14{\%} in the Pigpa1-silenced mutants, compared with 77{\%} in wild type, showing that virulence is severely impaired. The results prove that PiGPA1 is crucial for zoospore motility and for pathogenicity in an important oomycete plant pathogen.",
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author = "M Latijnhouwers and W Ligterink and VG Vleeshouwers and van, {West P} and F Govers",
note = "The heterotrimeric G‐protein pathway is a ubiquitous eukaryotic signalling module that is known to regulate growth and differentiation in many plant pathogens. We previously identified Pigpa1, a gene encoding a G‐protein α subunit from the potato late blight pathogen Phytophthora infestans. P. infestans belongs to the class oomycetes, a group of organisms in which signal transduction processes have not yet been studied at the molecular level. To elucidate the function of Pigpa1, PiGPA1‐deficient mutants were obtained by homology‐dependent gene silencing. The Pigpa1‐silenced mutants produced zoospores that turned six to eight times more frequently, causing them to swim only short distances compared with wild type. Attraction to the surface, a phenomenon known as negative geotaxis, was impaired in the mutant zoospores, as well as autoaggregation and chemotaxis towards glutamic and aspartic acid. Zoospore production was reduced by 20–45{\%} in different Pigpa1‐silenced mutants. Transformants expressing constitutively active forms of PiGPA1, containing amino acid substitutions (R177H and Q203L), showed no obvious phenotypic differences from the wild‐type strain. Infection efficiencies on potato leaves ranged from 3{\%} to 14{\%} in the Pigpa1‐silenced mutants, compared with 77{\%} in wild type, showing that virulence is severely impaired. The results prove that PiGPA1 is crucial for zoospore motility and for pathogenicity in an important oomycete plant pathogen.",
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AU - van, West P

AU - Govers, F

N1 - The heterotrimeric G‐protein pathway is a ubiquitous eukaryotic signalling module that is known to regulate growth and differentiation in many plant pathogens. We previously identified Pigpa1, a gene encoding a G‐protein α subunit from the potato late blight pathogen Phytophthora infestans. P. infestans belongs to the class oomycetes, a group of organisms in which signal transduction processes have not yet been studied at the molecular level. To elucidate the function of Pigpa1, PiGPA1‐deficient mutants were obtained by homology‐dependent gene silencing. The Pigpa1‐silenced mutants produced zoospores that turned six to eight times more frequently, causing them to swim only short distances compared with wild type. Attraction to the surface, a phenomenon known as negative geotaxis, was impaired in the mutant zoospores, as well as autoaggregation and chemotaxis towards glutamic and aspartic acid. Zoospore production was reduced by 20–45% in different Pigpa1‐silenced mutants. Transformants expressing constitutively active forms of PiGPA1, containing amino acid substitutions (R177H and Q203L), showed no obvious phenotypic differences from the wild‐type strain. Infection efficiencies on potato leaves ranged from 3% to 14% in the Pigpa1‐silenced mutants, compared with 77% in wild type, showing that virulence is severely impaired. The results prove that PiGPA1 is crucial for zoospore motility and for pathogenicity in an important oomycete plant pathogen.

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KW - heterotrimeric G-proteins

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KW - arabidopsis-thaliana

KW - fungus phytophthora

KW - cryphonectria-parasitica

KW - signal-transduction

KW - cell-proliferation

KW - neurospora-crassa

KW - gamma-subunit

KW - genes

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JO - Molecular Microbiology

JF - Molecular Microbiology

SN - 0950-382X

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