A High incidence of clustered microsatellite mutations revealed by parent-offpring analysis in an African freshwater snail, Bulinus forskalii (Gastropoda, Pulmonata).

Jennifer Gow, Leslie Robert Noble, D. Rollinson, Catherine Sue Jones

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Genotyping of 11 microsatellites in 432 offspring from 28 families of the hermaphroditic, freshwater snail Bulinus forskalii detected 10 de novo mutant alleles. This gave an estimated mutation rate of 1.1 X 10(-3) per locus per gamete per generation. There was a trend towards repeat length expansion and, unlike most studies, multi-step mutations predominated, suggesting that the microsatellite mutation process does not conform to a strict stepwise mutation model. Interestingly, the ten mutant alleles appear to have arisen from only six independent germline mutation events within the microsatellite array, with seven of them residing in three mutational clusters. Our results extend observations of clustered microsatellite mutations to another taxonomic group and type of mating system, self-fertile gastropods, and provide compelling evidence of premeiotic germ,line mutations, a phenomenon that could greatly impact upon our understanding of mutation dynamics but which has received little attention.

Original languageEnglish
Pages (from-to)77-83
Number of pages6
JournalGenetica
Volume124
DOIs
Publication statusPublished - 2005

Keywords

  • Bulinus forskalii
  • freshwater snail
  • germline mosaicism
  • microsatellite
  • premeiotic mutation
  • DNA-SEQUENCE
  • LOCI
  • POPULATION
  • RATES
  • MELANOGASTER
  • EVOLUTION
  • PATERNITY
  • ALLELES
  • REPEATS
  • COMPLEX

Cite this

A High incidence of clustered microsatellite mutations revealed by parent-offpring analysis in an African freshwater snail, Bulinus forskalii (Gastropoda, Pulmonata). / Gow, Jennifer; Noble, Leslie Robert; Rollinson, D.; Jones, Catherine Sue.

In: Genetica, Vol. 124, 2005, p. 77-83.

Research output: Contribution to journalArticle

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abstract = "Genotyping of 11 microsatellites in 432 offspring from 28 families of the hermaphroditic, freshwater snail Bulinus forskalii detected 10 de novo mutant alleles. This gave an estimated mutation rate of 1.1 X 10(-3) per locus per gamete per generation. There was a trend towards repeat length expansion and, unlike most studies, multi-step mutations predominated, suggesting that the microsatellite mutation process does not conform to a strict stepwise mutation model. Interestingly, the ten mutant alleles appear to have arisen from only six independent germline mutation events within the microsatellite array, with seven of them residing in three mutational clusters. Our results extend observations of clustered microsatellite mutations to another taxonomic group and type of mating system, self-fertile gastropods, and provide compelling evidence of premeiotic germ,line mutations, a phenomenon that could greatly impact upon our understanding of mutation dynamics but which has received little attention.",
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AU - Noble, Leslie Robert

AU - Rollinson, D.

AU - Jones, Catherine Sue

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AB - Genotyping of 11 microsatellites in 432 offspring from 28 families of the hermaphroditic, freshwater snail Bulinus forskalii detected 10 de novo mutant alleles. This gave an estimated mutation rate of 1.1 X 10(-3) per locus per gamete per generation. There was a trend towards repeat length expansion and, unlike most studies, multi-step mutations predominated, suggesting that the microsatellite mutation process does not conform to a strict stepwise mutation model. Interestingly, the ten mutant alleles appear to have arisen from only six independent germline mutation events within the microsatellite array, with seven of them residing in three mutational clusters. Our results extend observations of clustered microsatellite mutations to another taxonomic group and type of mating system, self-fertile gastropods, and provide compelling evidence of premeiotic germ,line mutations, a phenomenon that could greatly impact upon our understanding of mutation dynamics but which has received little attention.

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KW - POPULATION

KW - RATES

KW - MELANOGASTER

KW - EVOLUTION

KW - PATERNITY

KW - ALLELES

KW - REPEATS

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