A human monoclonal antibody against HLA-Cw1 and a human monoclonal antibody against an HLA-A locus determinant derived from a single uniparous female

A Mulder, M J Kardol, C M Uit het Broek, J Tanke-Visser, Neil Thomas Young, F H Claas

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Two human monoclonal antibodies (HuMAbs) with widely different HLA specificities were raised from a uniparous HLA-seropositive female. Screening against a large panel of serologically HLA-typed lymphocytes in the complement-dependent cytotoxicity test showed that one of these HuMAbs, VP6G3, was specific for HLA-Cw1, thereby constituting the first HuMAb against an HLA-C locus product. The second HuMAb, VP5G3, was directed against an HLA-A-encoded determinant shared by HLA-A11, -A25, -A26 and -A66. The epitopes responsible for binding were determined by comparing the aminoacid sequences and were pinpointed to the 6K/9F combination for HuMAb VP6G3, and 163R with a critical contribution of aminoacids present at positions 166/167 for HuMAb VP5G3.
Original languageEnglish
Pages (from-to)393-396
Number of pages4
JournalTissue Antigens
Volume52
Issue number4
DOIs
Publication statusPublished - 1 Oct 1998

Keywords

  • Animals
  • Antibodies, Monoclonal
  • Antibody Specificity
  • B-Lymphocytes
  • Callithrix
  • Cell Line
  • Epitope Mapping
  • Female
  • Fetal Blood
  • HLA-A Antigens
  • HLA-C Antigens
  • Histocompatibility Testing
  • Humans
  • Immunodominant Epitopes
  • Parity

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