Abstract
Two human monoclonal antibodies (HuMAbs) with widely different HLA specificities were raised from a uniparous HLA-seropositive female. Screening against a large panel of serologically HLA-typed lymphocytes in the complement-dependent cytotoxicity test showed that one of these HuMAbs, VP6G3, was specific for HLA-Cw1, thereby constituting the first HuMAb against an HLA-C locus product. The second HuMAb, VP5G3, was directed against an HLA-A-encoded determinant shared by HLA-A11, -A25, -A26 and -A66. The epitopes responsible for binding were determined by comparing the aminoacid sequences and were pinpointed to the 6K/9F combination for HuMAb VP6G3, and 163R with a critical contribution of aminoacids present at positions 166/167 for HuMAb VP5G3.
Original language | English |
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Pages (from-to) | 393-396 |
Number of pages | 4 |
Journal | Tissue Antigens |
Volume | 52 |
Issue number | 4 |
DOIs | |
Publication status | Published - 1 Oct 1998 |
Keywords
- Animals
- Antibodies, Monoclonal
- Antibody Specificity
- B-Lymphocytes
- Callithrix
- Cell Line
- Epitope Mapping
- Female
- Fetal Blood
- HLA-A Antigens
- HLA-C Antigens
- Histocompatibility Testing
- Humans
- Immunodominant Epitopes
- Parity