A method of patch clamp recording in hyperbaric oxygen

C. V. Jamieson, Alistar Gordon MacDonald

    Research output: Contribution to journalArticle

    2 Citations (Scopus)

    Abstract

    Jamieson, CV, MacDonald, AG, A method of patch clamp recording in hyperbaric oxygen. Undersea Hyperb Med 2002; 29(4):286-293- A method of patch clamp recording in hyperbaric oxygen is described which is derived from a method for patch clamping at high hydrostatic pressure. Excised patches equilibrate with hyperbaric oxygen within 10 minutes. Experiments with BK channels expressed in HEK 293 cells showed that 1 MPa O-2 caused an increase in the ionic permeability of the patch, an effect accelerated in the presence of ferrous ions. Seal resistance was unaffected. Small negative currents, (V-H - 40 to - 65 mV, symmetrical KCl solutions), apparent in skewed all-points amplitude histograms, reduced the signal to noise ratio. In some patches the BK channel activity was inhibited and in others it persisted with the currents becoming irresolvable in the increased noise.

    Original languageEnglish
    Pages (from-to)286-293
    Number of pages7
    JournalUndersea & Hyperbaric Medicine
    Volume29
    Issue number4
    Publication statusPublished - 2002

    Keywords

    • oxygen toxicity
    • free radicals
    • BK channel
    • patch clamp
    • lipid-peroxidation
    • rat-brain
    • proteins
    • convulsions
    • oxidation
    • liposomes
    • exposure
    • cells
    • ion

    Cite this

    Jamieson, C. V., & MacDonald, A. G. (2002). A method of patch clamp recording in hyperbaric oxygen. Undersea & Hyperbaric Medicine, 29(4), 286-293.