@article{854b0f9b190c44ccba4a0f534e0e311a,
title = "A mouse line for inducible and reversible silencing of specific neurons",
abstract = "Background: Genetic methods for inducibly and reversibly inhibiting neuronal activity of specific neurons are critical for exploring the functions of neuronal circuits. The engineered human glycine receptor, called ivermectin (IVM)-gated silencing receptor (IVMR), has been shown to possess this ability in vitro.Results: Here we generated a mouse line, in which the IVMR coding sequence was inserted into the ROSA26 locus downstream of a loxP-flanked STOP cassette. Specific Cre-mediated IVMR expression was revealed by mis-expression of Cre in the striatum and by crossing with several Cre lines. Behavioral alteration was observed in Rosa26-IVMR mice with unilateral striatal Cre expression after systemic administration of IVM, and it could be re-initiated when IVM was applied again. A dramatic reduction in neuron firing was recorded in IVM-treated free moving Rosa26-IVMR; Emx1-Cre mice, and neuronal excitability was reduced within minutes as shown by recording in brain slice.Conclusion: This Rosa26-IVMR mouse line provides a powerful tool for exploring selective circuit functions in freely behaving mice.",
keywords = "Neuron silencing, Ligand-gated channel, Ivermectin, Rosa26, mammalian neurons, neural circuits, ivermectin, mice, receptor, inactivation, expression, drosphilia, vectors, pathway",
author = "Ling Hu and Wei Lan and Hao Guo and Guo-Dong Chai and Kun Huang and Ling Zhang and Ying Huang and Xue-Feng Chen and Lei Zhang and Ning-Ning Song and Ling Chen and Bing Lang and Yun Wang and Qing-Xiu Wang and Jin-Bao Zhang and Collin McCaig and Lin Xu and Yu-Qiang Ding",
note = "This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. Acknowledgements: We thank Dr. Joseph W. Lynch for sharing the IVMR plasmid, and Dr. Lisa M. Monteggia for sharing the AAV2-Cre plasmid. Rosa-CAG targeting vector was obtained from Addgenes. This work was supported by the Key State Research Program from Ministry of Science and Technology of China (2011CB510005, 2012CB966900, 2013CB835103), National Natural Science Foundation of China (81221001, 81200692, 81101026, 31100788, 31271182, 31030034, 91232724), Science and Technology Commission of Shanghai Municipality (12XD1404800), Shanghai Pujiang Program (12PJ1408800), Key Disciplines Group Construction Project of Pudong Health Bureau of Shanghai (PWZxq2014-04) and Sino-UK Higher Education Research Partnership for PhD Studies.",
year = "2014",
month = sep,
day = "18",
doi = "10.1186/s13041-014-0068-8",
language = "English",
volume = "7",
journal = "Molecular brain",
issn = "1756-6606",
publisher = "BioMed Central",
}