A NEW ESCHERICHIA-COLI - BACTEROIDES SHUTTLE VECTOR, PRRI207, BASED ON THE BACTEROIDES-RUMINICOLA PLASMID REPLICON PRRI2

A M THOMSON, H J FLINT, M BECHET, J MARTIN, H C DUBOURGUIER

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

A 3.4kb cryptic plasmid, pRRI2, was isolated from Bacteroides ruminicola 223/M2/7 and used as the basis for a new Bacteriodes/Escherichia shuttle vector. Constructs were made incorporating pRRI2, a Bacteroides erythromycin/clindamycin resistance marker and the E. coli pUC8-derived vector pHG165. One of these, pRRI207 (11 kb), was capable of introduction into strains belonging to four different species of Bacteroides (B. uniformis, B. distasonis, B. thetaiotaomicron, or B. ruminicola) either by conjugal transfer from E. coli or by electrotransformation. pRRI207 carries several unique restriction sites derived from the pUC8 multiple cloning site. Only one of six B. ruminicola strains tested was used successfully as a recipient for pRRI207, indicating that further modifications to transfer procedures or marker genes may be needed for wider application in this species.

Original languageEnglish
Pages (from-to)49-54
Number of pages6
JournalCurrent Microbiology
Volume24
Issue number1
Publication statusPublished - Jan 1992

Keywords

  • CLONING
  • EXPRESSION
  • FRAGILIS
  • R751

Cite this

A NEW ESCHERICHIA-COLI - BACTEROIDES SHUTTLE VECTOR, PRRI207, BASED ON THE BACTEROIDES-RUMINICOLA PLASMID REPLICON PRRI2. / THOMSON, A M ; FLINT, H J ; BECHET, M ; MARTIN, J ; DUBOURGUIER, H C .

In: Current Microbiology, Vol. 24, No. 1, 01.1992, p. 49-54.

Research output: Contribution to journalArticle

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abstract = "A 3.4kb cryptic plasmid, pRRI2, was isolated from Bacteroides ruminicola 223/M2/7 and used as the basis for a new Bacteriodes/Escherichia shuttle vector. Constructs were made incorporating pRRI2, a Bacteroides erythromycin/clindamycin resistance marker and the E. coli pUC8-derived vector pHG165. One of these, pRRI207 (11 kb), was capable of introduction into strains belonging to four different species of Bacteroides (B. uniformis, B. distasonis, B. thetaiotaomicron, or B. ruminicola) either by conjugal transfer from E. coli or by electrotransformation. pRRI207 carries several unique restriction sites derived from the pUC8 multiple cloning site. Only one of six B. ruminicola strains tested was used successfully as a recipient for pRRI207, indicating that further modifications to transfer procedures or marker genes may be needed for wider application in this species.",
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AB - A 3.4kb cryptic plasmid, pRRI2, was isolated from Bacteroides ruminicola 223/M2/7 and used as the basis for a new Bacteriodes/Escherichia shuttle vector. Constructs were made incorporating pRRI2, a Bacteroides erythromycin/clindamycin resistance marker and the E. coli pUC8-derived vector pHG165. One of these, pRRI207 (11 kb), was capable of introduction into strains belonging to four different species of Bacteroides (B. uniformis, B. distasonis, B. thetaiotaomicron, or B. ruminicola) either by conjugal transfer from E. coli or by electrotransformation. pRRI207 carries several unique restriction sites derived from the pUC8 multiple cloning site. Only one of six B. ruminicola strains tested was used successfully as a recipient for pRRI207, indicating that further modifications to transfer procedures or marker genes may be needed for wider application in this species.

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