A new family of heparin binding growth/differentiation factors

differential expression of the midkine (MK) and HB-GAM genes during mouse development

Masaru Nakamoto, S Matsubara, Teruo Miyauchi, Hiroya Obama, Masayuki Ozawa, Takashi Muramatsu

Research output: Contribution to journalArticle

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Abstract

MK (midkine) and HB-GAM (heparin-binding growth-associated molecule) constitute a
new family of heparin-binding growth differentiation factors. The modes of expression of
MK and HB-GAM during mouse development were quantitatively examined by mRNA
hybridization. The following three distinct patterns of expression were observed in the
brain/head region. On the llth-13th days of gestation, MK was intensely, but HB-GAM
relatively weakly expressed; on the 15th-19th days, both MK and HB-GAM expression
became weaker; and in the neonatal period, HB-GAM was intensely expressed and MK
expression increased slightly. The level of HB-GAM expression was lower than that of MK
in the whole embryo on the 11th to 13th days of gestation. HB-GAM mRNA was detected in
the kidney of newborn and young mice, where MK was more highly expressed. The identity
of the weakly expressed MK and HB-GAM signals was confirmed by means of the
polymerase chain reaction in the neonatal brain (MK), the head of 13-day embryos
(HB-GAM), and the kidney of 7-day-old mice (HB-GAM). In conclusion, MK and HB-GAM
are frequently co-expressed in the same cells and anatomic regions of the fetus or new born
mouse, while their modes of expression differ.
Original languageEnglish
Pages (from-to)346-349
Number of pages4
JournalThe Journal of Biochemistry
Volume112
Issue number3
Publication statusPublished - Sep 1992

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Growth Differentiation Factors
Heparin
Genes
Embryonic Structures
Head
Kidney
midkine
pleiotrophin
Pregnancy
Brain
Fetus

Cite this

A new family of heparin binding growth/differentiation factors : differential expression of the midkine (MK) and HB-GAM genes during mouse development. / Nakamoto, Masaru; Matsubara, S; Miyauchi, Teruo; Obama, Hiroya; Ozawa, Masayuki; Muramatsu, Takashi.

In: The Journal of Biochemistry, Vol. 112, No. 3, 09.1992, p. 346-349.

Research output: Contribution to journalArticle

Nakamoto, Masaru ; Matsubara, S ; Miyauchi, Teruo ; Obama, Hiroya ; Ozawa, Masayuki ; Muramatsu, Takashi. / A new family of heparin binding growth/differentiation factors : differential expression of the midkine (MK) and HB-GAM genes during mouse development. In: The Journal of Biochemistry. 1992 ; Vol. 112, No. 3. pp. 346-349.
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title = "A new family of heparin binding growth/differentiation factors: differential expression of the midkine (MK) and HB-GAM genes during mouse development",
abstract = "MK (midkine) and HB-GAM (heparin-binding growth-associated molecule) constitute anew family of heparin-binding growth differentiation factors. The modes of expression ofMK and HB-GAM during mouse development were quantitatively examined by mRNAhybridization. The following three distinct patterns of expression were observed in thebrain/head region. On the llth-13th days of gestation, MK was intensely, but HB-GAMrelatively weakly expressed; on the 15th-19th days, both MK and HB-GAM expressionbecame weaker; and in the neonatal period, HB-GAM was intensely expressed and MKexpression increased slightly. The level of HB-GAM expression was lower than that of MKin the whole embryo on the 11th to 13th days of gestation. HB-GAM mRNA was detected inthe kidney of newborn and young mice, where MK was more highly expressed. The identityof the weakly expressed MK and HB-GAM signals was confirmed by means of thepolymerase chain reaction in the neonatal brain (MK), the head of 13-day embryos(HB-GAM), and the kidney of 7-day-old mice (HB-GAM). In conclusion, MK and HB-GAMare frequently co-expressed in the same cells and anatomic regions of the fetus or new bornmouse, while their modes of expression differ.",
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T1 - A new family of heparin binding growth/differentiation factors

T2 - differential expression of the midkine (MK) and HB-GAM genes during mouse development

AU - Nakamoto, Masaru

AU - Matsubara, S

AU - Miyauchi, Teruo

AU - Obama, Hiroya

AU - Ozawa, Masayuki

AU - Muramatsu, Takashi

PY - 1992/9

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N2 - MK (midkine) and HB-GAM (heparin-binding growth-associated molecule) constitute anew family of heparin-binding growth differentiation factors. The modes of expression ofMK and HB-GAM during mouse development were quantitatively examined by mRNAhybridization. The following three distinct patterns of expression were observed in thebrain/head region. On the llth-13th days of gestation, MK was intensely, but HB-GAMrelatively weakly expressed; on the 15th-19th days, both MK and HB-GAM expressionbecame weaker; and in the neonatal period, HB-GAM was intensely expressed and MKexpression increased slightly. The level of HB-GAM expression was lower than that of MKin the whole embryo on the 11th to 13th days of gestation. HB-GAM mRNA was detected inthe kidney of newborn and young mice, where MK was more highly expressed. The identityof the weakly expressed MK and HB-GAM signals was confirmed by means of thepolymerase chain reaction in the neonatal brain (MK), the head of 13-day embryos(HB-GAM), and the kidney of 7-day-old mice (HB-GAM). In conclusion, MK and HB-GAMare frequently co-expressed in the same cells and anatomic regions of the fetus or new bornmouse, while their modes of expression differ.

AB - MK (midkine) and HB-GAM (heparin-binding growth-associated molecule) constitute anew family of heparin-binding growth differentiation factors. The modes of expression ofMK and HB-GAM during mouse development were quantitatively examined by mRNAhybridization. The following three distinct patterns of expression were observed in thebrain/head region. On the llth-13th days of gestation, MK was intensely, but HB-GAMrelatively weakly expressed; on the 15th-19th days, both MK and HB-GAM expressionbecame weaker; and in the neonatal period, HB-GAM was intensely expressed and MKexpression increased slightly. The level of HB-GAM expression was lower than that of MKin the whole embryo on the 11th to 13th days of gestation. HB-GAM mRNA was detected inthe kidney of newborn and young mice, where MK was more highly expressed. The identityof the weakly expressed MK and HB-GAM signals was confirmed by means of thepolymerase chain reaction in the neonatal brain (MK), the head of 13-day embryos(HB-GAM), and the kidney of 7-day-old mice (HB-GAM). In conclusion, MK and HB-GAMare frequently co-expressed in the same cells and anatomic regions of the fetus or new bornmouse, while their modes of expression differ.

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EP - 349

JO - The Journal of Biochemistry

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