Abstract
MK (midkine) and HB-GAM (heparin-binding growth-associated molecule) constitute a
new family of heparin-binding growth differentiation factors. The modes of expression of
MK and HB-GAM during mouse development were quantitatively examined by mRNA
hybridization. The following three distinct patterns of expression were observed in the
brain/head region. On the llth-13th days of gestation, MK was intensely, but HB-GAM
relatively weakly expressed; on the 15th-19th days, both MK and HB-GAM expression
became weaker; and in the neonatal period, HB-GAM was intensely expressed and MK
expression increased slightly. The level of HB-GAM expression was lower than that of MK
in the whole embryo on the 11th to 13th days of gestation. HB-GAM mRNA was detected in
the kidney of newborn and young mice, where MK was more highly expressed. The identity
of the weakly expressed MK and HB-GAM signals was confirmed by means of the
polymerase chain reaction in the neonatal brain (MK), the head of 13-day embryos
(HB-GAM), and the kidney of 7-day-old mice (HB-GAM). In conclusion, MK and HB-GAM
are frequently co-expressed in the same cells and anatomic regions of the fetus or new born
mouse, while their modes of expression differ.
new family of heparin-binding growth differentiation factors. The modes of expression of
MK and HB-GAM during mouse development were quantitatively examined by mRNA
hybridization. The following three distinct patterns of expression were observed in the
brain/head region. On the llth-13th days of gestation, MK was intensely, but HB-GAM
relatively weakly expressed; on the 15th-19th days, both MK and HB-GAM expression
became weaker; and in the neonatal period, HB-GAM was intensely expressed and MK
expression increased slightly. The level of HB-GAM expression was lower than that of MK
in the whole embryo on the 11th to 13th days of gestation. HB-GAM mRNA was detected in
the kidney of newborn and young mice, where MK was more highly expressed. The identity
of the weakly expressed MK and HB-GAM signals was confirmed by means of the
polymerase chain reaction in the neonatal brain (MK), the head of 13-day embryos
(HB-GAM), and the kidney of 7-day-old mice (HB-GAM). In conclusion, MK and HB-GAM
are frequently co-expressed in the same cells and anatomic regions of the fetus or new born
mouse, while their modes of expression differ.
Original language | English |
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Pages (from-to) | 346-349 |
Number of pages | 4 |
Journal | The Journal of Biochemistry |
Volume | 112 |
Issue number | 3 |
Publication status | Published - Sept 1992 |