A new family of heparin binding growth/differentiation factors: differential expression of the midkine (MK) and HB-GAM genes during mouse development

Masaru Nakamoto, S Matsubara, Teruo Miyauchi, Hiroya Obama, Masayuki Ozawa, Takashi Muramatsu

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

MK (midkine) and HB-GAM (heparin-binding growth-associated molecule) constitute a
new family of heparin-binding growth differentiation factors. The modes of expression of
MK and HB-GAM during mouse development were quantitatively examined by mRNA
hybridization. The following three distinct patterns of expression were observed in the
brain/head region. On the llth-13th days of gestation, MK was intensely, but HB-GAM
relatively weakly expressed; on the 15th-19th days, both MK and HB-GAM expression
became weaker; and in the neonatal period, HB-GAM was intensely expressed and MK
expression increased slightly. The level of HB-GAM expression was lower than that of MK
in the whole embryo on the 11th to 13th days of gestation. HB-GAM mRNA was detected in
the kidney of newborn and young mice, where MK was more highly expressed. The identity
of the weakly expressed MK and HB-GAM signals was confirmed by means of the
polymerase chain reaction in the neonatal brain (MK), the head of 13-day embryos
(HB-GAM), and the kidney of 7-day-old mice (HB-GAM). In conclusion, MK and HB-GAM
are frequently co-expressed in the same cells and anatomic regions of the fetus or new born
mouse, while their modes of expression differ.
Original languageEnglish
Pages (from-to)346-349
Number of pages4
JournalThe Journal of Biochemistry
Volume112
Issue number3
Publication statusPublished - Sep 1992

Fingerprint Dive into the research topics of 'A new family of heparin binding growth/differentiation factors: differential expression of the midkine (MK) and HB-GAM genes during mouse development'. Together they form a unique fingerprint.

Cite this