A new model of corneal transplantation in the miniature pig: efficacy of immunosuppressive treatment

Urania Tavandzi, Radek Prochazka, Dusan Usvald, Jana Hlucilova, Martina Vitaskova, Jan Motlik, Andrea Vitova, Martin Filipec, John V. Forrester, Vladimir Holan

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8 Citations (Scopus)

Abstract

Corneal allograft rejection is frequently studied in small rodent or rabbit models. To study mechanisms of rejection in a model that more closely mimics transplantation in humans, we performed orthotopic corneal transplantation in the miniature pig using a 7-mm diameter donor graft. Four groups of recipients were studied: 1) untreated naive, 2) untreated vascularized (high risk), 3) high-risk grafts treated by topical application of prednisolone, or 4) high-risk grafts treated with a combined systemic immunosuppression regime of oral prednisone, cyclosporine A, and mycophenolate mofetil. Both the clinical features and histological assessment of corneal graft rejection showed close similarities to graft rejection in humans. Interestingly, preliminary results indicated that topical steroid treatment was superior to systemic immunosuppression in significantly promoting graft survival. Thus, corneal transplantation in the pig represents an animal model most closely resembling corneal grafting in humans, and offers possibilities for testing various clinically applicable immunosuppressive treatments.

Original languageEnglish
Pages (from-to)1401-1403
Number of pages3
JournalTransplantation
Volume83
Issue number10
DOIs
Publication statusPublished - 27 May 2007

Keywords

  • pigs
  • corneal transplantation
  • high-risk recipients
  • immunosuppression
  • high-risk keratoplasty
  • penetrating keratoplasty
  • graft-rejection
  • mycophenolate-mofetil
  • allograft survival
  • mouse model
  • mice
  • cyclosporine
  • prevention
  • sheep

Cite this

Tavandzi, U., Prochazka, R., Usvald, D., Hlucilova, J., Vitaskova, M., Motlik, J., Vitova, A., Filipec, M., Forrester, J. V., & Holan, V. (2007). A new model of corneal transplantation in the miniature pig: efficacy of immunosuppressive treatment. Transplantation, 83(10), 1401-1403. https://doi.org/10.1097/01.tp.0000261711.08833.a1