A new model of posterior capsule opacification in rodents

Noemi Lois, Rosemary Anne Dawson, Alastair D McKinnon, John Vincent Forrester

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

PURPOSE. To describe a new model of posterior capsule opacification (PCO) in rodents

METHODS. An extracapsular lens extraction (ECLE), by continuous curvilinear capsulorrhexis and hydrodissection, was performed in 42 consecutive Brown Norway rats. Animals were killed at 0, 6, and 24 hours and 3, 7, and 14 days after surgery. Eyes were enucleated and processed for light microscopy and immunohistochemistry.

RESULTS. In 34 (81%) of the animals the operated eye appeared well healed before death, with a clear cornea and a well-formed anterior chamber. In eight (19%) there was no view of anterior segment structures because of hyphema, fibrin, or corneal opacification. PCO was clinically evident 3 days after ECLE and was present in all animals at 2 weeks. Immediately after ECLE, lens epithelial cells (LECs) were present in the inner surface of the anterior capsule and lens bow. Twenty-four hours after surgery, LECs started to migrate toward the center of the posterior capsule. At 3 days, multilayered LECs, some spindle shaped, were present throughout the lens capsule. Capsular wrinkling was apparent. Lens fibers and Soemmering's ring were observed in all animals 14 days after surgery, indicating some degree of cellular differentiation. Activated macrophages were present in greater numbers at 3 and 14 days after surgery (P < 0.05), when proliferation and migration of LECs appeared to be greatest, and lens fiber differentiation was evident, respectively.

CONCLUSIONS. In rodents PCO occurs after ECLE and is associated with low-grade inflammation, mostly of mononuclear macrophages. Although no intraocular lens implantation was performed, this model appears to be valuable for studying the sequence of events that leads to PCO after cataract surgery and the extracellular matrix cues that promote lens fiber differentiation.

Original languageEnglish
Pages (from-to)3450-3457
Number of pages7
JournalInvestigative Ophthalmology & Visual Science
Volume44
Issue number8
DOIs
Publication statusPublished - 2003

Keywords

  • LENS EPITHELIAL-CELLS
  • EXTRACAPSULAR CATARACT-EXTRACTION
  • SMOOTH MUSCLE ACTIN
  • TGF-BETA
  • POSTOPERATIVE INFLAMMATION
  • COLLAGEN-SYNTHESIS
  • DICLOFENAC SODIUM
  • PSEUDOPHAKIC EYES
  • GROWTH-FACTORS
  • YAG LASER

Cite this

A new model of posterior capsule opacification in rodents. / Lois, Noemi; Dawson, Rosemary Anne; McKinnon, Alastair D; Forrester, John Vincent.

In: Investigative Ophthalmology & Visual Science, Vol. 44, No. 8, 2003, p. 3450-3457.

Research output: Contribution to journalArticle

Lois, Noemi ; Dawson, Rosemary Anne ; McKinnon, Alastair D ; Forrester, John Vincent. / A new model of posterior capsule opacification in rodents. In: Investigative Ophthalmology & Visual Science. 2003 ; Vol. 44, No. 8. pp. 3450-3457.
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abstract = "PURPOSE. To describe a new model of posterior capsule opacification (PCO) in rodentsMETHODS. An extracapsular lens extraction (ECLE), by continuous curvilinear capsulorrhexis and hydrodissection, was performed in 42 consecutive Brown Norway rats. Animals were killed at 0, 6, and 24 hours and 3, 7, and 14 days after surgery. Eyes were enucleated and processed for light microscopy and immunohistochemistry.RESULTS. In 34 (81{\%}) of the animals the operated eye appeared well healed before death, with a clear cornea and a well-formed anterior chamber. In eight (19{\%}) there was no view of anterior segment structures because of hyphema, fibrin, or corneal opacification. PCO was clinically evident 3 days after ECLE and was present in all animals at 2 weeks. Immediately after ECLE, lens epithelial cells (LECs) were present in the inner surface of the anterior capsule and lens bow. Twenty-four hours after surgery, LECs started to migrate toward the center of the posterior capsule. At 3 days, multilayered LECs, some spindle shaped, were present throughout the lens capsule. Capsular wrinkling was apparent. Lens fibers and Soemmering's ring were observed in all animals 14 days after surgery, indicating some degree of cellular differentiation. Activated macrophages were present in greater numbers at 3 and 14 days after surgery (P < 0.05), when proliferation and migration of LECs appeared to be greatest, and lens fiber differentiation was evident, respectively.CONCLUSIONS. In rodents PCO occurs after ECLE and is associated with low-grade inflammation, mostly of mononuclear macrophages. Although no intraocular lens implantation was performed, this model appears to be valuable for studying the sequence of events that leads to PCO after cataract surgery and the extracellular matrix cues that promote lens fiber differentiation.",
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TY - JOUR

T1 - A new model of posterior capsule opacification in rodents

AU - Lois, Noemi

AU - Dawson, Rosemary Anne

AU - McKinnon, Alastair D

AU - Forrester, John Vincent

PY - 2003

Y1 - 2003

N2 - PURPOSE. To describe a new model of posterior capsule opacification (PCO) in rodentsMETHODS. An extracapsular lens extraction (ECLE), by continuous curvilinear capsulorrhexis and hydrodissection, was performed in 42 consecutive Brown Norway rats. Animals were killed at 0, 6, and 24 hours and 3, 7, and 14 days after surgery. Eyes were enucleated and processed for light microscopy and immunohistochemistry.RESULTS. In 34 (81%) of the animals the operated eye appeared well healed before death, with a clear cornea and a well-formed anterior chamber. In eight (19%) there was no view of anterior segment structures because of hyphema, fibrin, or corneal opacification. PCO was clinically evident 3 days after ECLE and was present in all animals at 2 weeks. Immediately after ECLE, lens epithelial cells (LECs) were present in the inner surface of the anterior capsule and lens bow. Twenty-four hours after surgery, LECs started to migrate toward the center of the posterior capsule. At 3 days, multilayered LECs, some spindle shaped, were present throughout the lens capsule. Capsular wrinkling was apparent. Lens fibers and Soemmering's ring were observed in all animals 14 days after surgery, indicating some degree of cellular differentiation. Activated macrophages were present in greater numbers at 3 and 14 days after surgery (P < 0.05), when proliferation and migration of LECs appeared to be greatest, and lens fiber differentiation was evident, respectively.CONCLUSIONS. In rodents PCO occurs after ECLE and is associated with low-grade inflammation, mostly of mononuclear macrophages. Although no intraocular lens implantation was performed, this model appears to be valuable for studying the sequence of events that leads to PCO after cataract surgery and the extracellular matrix cues that promote lens fiber differentiation.

AB - PURPOSE. To describe a new model of posterior capsule opacification (PCO) in rodentsMETHODS. An extracapsular lens extraction (ECLE), by continuous curvilinear capsulorrhexis and hydrodissection, was performed in 42 consecutive Brown Norway rats. Animals were killed at 0, 6, and 24 hours and 3, 7, and 14 days after surgery. Eyes were enucleated and processed for light microscopy and immunohistochemistry.RESULTS. In 34 (81%) of the animals the operated eye appeared well healed before death, with a clear cornea and a well-formed anterior chamber. In eight (19%) there was no view of anterior segment structures because of hyphema, fibrin, or corneal opacification. PCO was clinically evident 3 days after ECLE and was present in all animals at 2 weeks. Immediately after ECLE, lens epithelial cells (LECs) were present in the inner surface of the anterior capsule and lens bow. Twenty-four hours after surgery, LECs started to migrate toward the center of the posterior capsule. At 3 days, multilayered LECs, some spindle shaped, were present throughout the lens capsule. Capsular wrinkling was apparent. Lens fibers and Soemmering's ring were observed in all animals 14 days after surgery, indicating some degree of cellular differentiation. Activated macrophages were present in greater numbers at 3 and 14 days after surgery (P < 0.05), when proliferation and migration of LECs appeared to be greatest, and lens fiber differentiation was evident, respectively.CONCLUSIONS. In rodents PCO occurs after ECLE and is associated with low-grade inflammation, mostly of mononuclear macrophages. Although no intraocular lens implantation was performed, this model appears to be valuable for studying the sequence of events that leads to PCO after cataract surgery and the extracellular matrix cues that promote lens fiber differentiation.

KW - LENS EPITHELIAL-CELLS

KW - EXTRACAPSULAR CATARACT-EXTRACTION

KW - SMOOTH MUSCLE ACTIN

KW - TGF-BETA

KW - POSTOPERATIVE INFLAMMATION

KW - COLLAGEN-SYNTHESIS

KW - DICLOFENAC SODIUM

KW - PSEUDOPHAKIC EYES

KW - GROWTH-FACTORS

KW - YAG LASER

U2 - 10.1167/iovs.02-1293

DO - 10.1167/iovs.02-1293

M3 - Article

VL - 44

SP - 3450

EP - 3457

JO - Investigative Ophthalmology & Visual Science

JF - Investigative Ophthalmology & Visual Science

SN - 0146-0404

IS - 8

ER -