A non-invasive approach to monitor chronic lymphocytic leukemia engraftment in a xenograft mouse model using ultra-small superparamagnetic iron oxide-magnetic resonance imaging (USPIO-MRI).

Francesca Valdora, Giovanna Cutrona, Serena Matis, Fortunato Morabito, Carlotta Massucco, Laura Emionite, Simona Boccardo, Luca Basso, Anna Grazia Recchia, Sandra Salvi, Francesca Rosa, Massimo Gentile, Marco Ravina, Daniele Pace, Angela Castronovo, Michele Cilli, Mauro Truini, Massimo Calabrese, Antonino Nerih, Carlo Emanuele NeumaierFranco Fais, Gabriella Baio

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Abstract

Chronic lymphocytic leukemia (CLL) is the most prevalent leukemia among adults. Despite its indolent nature, CLL remains an incurable disease. Herein we aimed to monitor CLL disease engraftment and,progression/regression in a xenograft CLL mouse model using ultra-small superparamagnetic iron oxide-magnetic resonance imaging (USPIO-MRI). Spleen contrast enhancement, quantified as percentage change in signal intensity upon USPIO administration, demonstrated a difference due to a reduced USPIO uptake, in the spleens of mice injected with CLL cells (NSG-CLL, n = 71) compared to controls (NSG-CTR, n = 17). These differences were statistically significant both after 2 and 4 weeks from CLL cells injection. In addition comparison of mice treated with rituximab with untreated controls for changes in spleen iron uptake confirmed that it is possible to monitor treatment efficacy in this mouse model of CLL using USPIO-enhanced MRI. Further applications could include the preclinical in vivo monitoring of new therapies and the clinical evaluation of CLL patients.
Original languageEnglish
Pages (from-to)52-60
Number of pages9
JournalClinical Immunology
Volume172
Early online date16 Jul 2016
DOIs
Publication statusPublished - Nov 2016

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B-Cell Chronic Lymphocytic Leukemia
Heterografts
Magnetic Resonance Imaging
Spleen
ferric oxide
Disease Progression
Leukemia
Iron
Injections

Keywords

  • Chronic Lymphocytic Leukemia (CLL)
  • magnetic resonance imaging (MRI)
  • ultra-small superparamagnetic iron oxide (USPIO)
  • Xenograft NSG mice model
  • disease monitoring

Cite this

A non-invasive approach to monitor chronic lymphocytic leukemia engraftment in a xenograft mouse model using ultra-small superparamagnetic iron oxide-magnetic resonance imaging (USPIO-MRI). / Valdora, Francesca ; Cutrona, Giovanna; Matis, Serena ; Morabito, Fortunato; Massucco, Carlotta; Emionite, Laura ; Boccardo, Simona; Basso, Luca; Recchia, Anna Grazia ; Salvi, Sandra; Rosa, Francesca; Gentile, Massimo ; Ravina, Marco ; Pace, Daniele; Castronovo, Angela ; Cilli, Michele ; Truini, Mauro; Calabrese, Massimo; Nerih, Antonino ; Neumaier, Carlo Emanuele; Fais, Franco ; Baio, Gabriella.

In: Clinical Immunology, Vol. 172, 11.2016, p. 52-60.

Research output: Contribution to journalArticle

Valdora, F, Cutrona, G, Matis, S, Morabito, F, Massucco, C, Emionite, L, Boccardo, S, Basso, L, Recchia, AG, Salvi, S, Rosa, F, Gentile, M, Ravina, M, Pace, D, Castronovo, A, Cilli, M, Truini, M, Calabrese, M, Nerih, A, Neumaier, CE, Fais, F & Baio, G 2016, 'A non-invasive approach to monitor chronic lymphocytic leukemia engraftment in a xenograft mouse model using ultra-small superparamagnetic iron oxide-magnetic resonance imaging (USPIO-MRI).', Clinical Immunology, vol. 172, pp. 52-60. https://doi.org/10.1016/j.clim.2016.07.013
Valdora, Francesca ; Cutrona, Giovanna ; Matis, Serena ; Morabito, Fortunato ; Massucco, Carlotta ; Emionite, Laura ; Boccardo, Simona ; Basso, Luca ; Recchia, Anna Grazia ; Salvi, Sandra ; Rosa, Francesca ; Gentile, Massimo ; Ravina, Marco ; Pace, Daniele ; Castronovo, Angela ; Cilli, Michele ; Truini, Mauro ; Calabrese, Massimo ; Nerih, Antonino ; Neumaier, Carlo Emanuele ; Fais, Franco ; Baio, Gabriella. / A non-invasive approach to monitor chronic lymphocytic leukemia engraftment in a xenograft mouse model using ultra-small superparamagnetic iron oxide-magnetic resonance imaging (USPIO-MRI). In: Clinical Immunology. 2016 ; Vol. 172. pp. 52-60.
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title = "A non-invasive approach to monitor chronic lymphocytic leukemia engraftment in a xenograft mouse model using ultra-small superparamagnetic iron oxide-magnetic resonance imaging (USPIO-MRI).",
abstract = "Chronic lymphocytic leukemia (CLL) is the most prevalent leukemia among adults. Despite its indolent nature, CLL remains an incurable disease. Herein we aimed to monitor CLL disease engraftment and,progression/regression in a xenograft CLL mouse model using ultra-small superparamagnetic iron oxide-magnetic resonance imaging (USPIO-MRI). Spleen contrast enhancement, quantified as percentage change in signal intensity upon USPIO administration, demonstrated a difference due to a reduced USPIO uptake, in the spleens of mice injected with CLL cells (NSG-CLL, n = 71) compared to controls (NSG-CTR, n = 17). These differences were statistically significant both after 2 and 4 weeks from CLL cells injection. In addition comparison of mice treated with rituximab with untreated controls for changes in spleen iron uptake confirmed that it is possible to monitor treatment efficacy in this mouse model of CLL using USPIO-enhanced MRI. Further applications could include the preclinical in vivo monitoring of new therapies and the clinical evaluation of CLL patients.",
keywords = "Chronic Lymphocytic Leukemia (CLL), magnetic resonance imaging (MRI), ultra-small superparamagnetic iron oxide (USPIO), Xenograft NSG mice model, disease monitoring",
author = "Francesca Valdora and Giovanna Cutrona and Serena Matis and Fortunato Morabito and Carlotta Massucco and Laura Emionite and Simona Boccardo and Luca Basso and Recchia, {Anna Grazia} and Sandra Salvi and Francesca Rosa and Massimo Gentile and Marco Ravina and Daniele Pace and Angela Castronovo and Michele Cilli and Mauro Truini and Massimo Calabrese and Antonino Nerih and Neumaier, {Carlo Emanuele} and Franco Fais and Gabriella Baio",
note = "This work was supported by: Associazione Italiana Ricerca sul Cancro (AIRC) [Grant 5 x mille n.9980, (to M.F., F.M. and A. N.)]; AIRC I.G. [n. 14,326 (to M.F.)], [n.10136 and 16,722 (A.N.)], [n.15426 (to F.F.)]. AIRC and Fondazione CaRiCal co-financed Multi Unit Regional Grant 2014 [n.16695 (to F.M.)]. Italian Ministry of Health 5 × 1000 funds (to F.F). A.G R. was supported by Associazione Italiana contro le Leucemie-Linfomi-Mielomi (AIL) Cosenza - Fondazione Amelia Scorza (FAS). S.M. C.M., F.V., L. E., S. B., were supported by AIRC.",
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T1 - A non-invasive approach to monitor chronic lymphocytic leukemia engraftment in a xenograft mouse model using ultra-small superparamagnetic iron oxide-magnetic resonance imaging (USPIO-MRI).

AU - Valdora, Francesca

AU - Cutrona, Giovanna

AU - Matis, Serena

AU - Morabito, Fortunato

AU - Massucco, Carlotta

AU - Emionite, Laura

AU - Boccardo, Simona

AU - Basso, Luca

AU - Recchia, Anna Grazia

AU - Salvi, Sandra

AU - Rosa, Francesca

AU - Gentile, Massimo

AU - Ravina, Marco

AU - Pace, Daniele

AU - Castronovo, Angela

AU - Cilli, Michele

AU - Truini, Mauro

AU - Calabrese, Massimo

AU - Nerih, Antonino

AU - Neumaier, Carlo Emanuele

AU - Fais, Franco

AU - Baio, Gabriella

N1 - This work was supported by: Associazione Italiana Ricerca sul Cancro (AIRC) [Grant 5 x mille n.9980, (to M.F., F.M. and A. N.)]; AIRC I.G. [n. 14,326 (to M.F.)], [n.10136 and 16,722 (A.N.)], [n.15426 (to F.F.)]. AIRC and Fondazione CaRiCal co-financed Multi Unit Regional Grant 2014 [n.16695 (to F.M.)]. Italian Ministry of Health 5 × 1000 funds (to F.F). A.G R. was supported by Associazione Italiana contro le Leucemie-Linfomi-Mielomi (AIL) Cosenza - Fondazione Amelia Scorza (FAS). S.M. C.M., F.V., L. E., S. B., were supported by AIRC.

PY - 2016/11

Y1 - 2016/11

N2 - Chronic lymphocytic leukemia (CLL) is the most prevalent leukemia among adults. Despite its indolent nature, CLL remains an incurable disease. Herein we aimed to monitor CLL disease engraftment and,progression/regression in a xenograft CLL mouse model using ultra-small superparamagnetic iron oxide-magnetic resonance imaging (USPIO-MRI). Spleen contrast enhancement, quantified as percentage change in signal intensity upon USPIO administration, demonstrated a difference due to a reduced USPIO uptake, in the spleens of mice injected with CLL cells (NSG-CLL, n = 71) compared to controls (NSG-CTR, n = 17). These differences were statistically significant both after 2 and 4 weeks from CLL cells injection. In addition comparison of mice treated with rituximab with untreated controls for changes in spleen iron uptake confirmed that it is possible to monitor treatment efficacy in this mouse model of CLL using USPIO-enhanced MRI. Further applications could include the preclinical in vivo monitoring of new therapies and the clinical evaluation of CLL patients.

AB - Chronic lymphocytic leukemia (CLL) is the most prevalent leukemia among adults. Despite its indolent nature, CLL remains an incurable disease. Herein we aimed to monitor CLL disease engraftment and,progression/regression in a xenograft CLL mouse model using ultra-small superparamagnetic iron oxide-magnetic resonance imaging (USPIO-MRI). Spleen contrast enhancement, quantified as percentage change in signal intensity upon USPIO administration, demonstrated a difference due to a reduced USPIO uptake, in the spleens of mice injected with CLL cells (NSG-CLL, n = 71) compared to controls (NSG-CTR, n = 17). These differences were statistically significant both after 2 and 4 weeks from CLL cells injection. In addition comparison of mice treated with rituximab with untreated controls for changes in spleen iron uptake confirmed that it is possible to monitor treatment efficacy in this mouse model of CLL using USPIO-enhanced MRI. Further applications could include the preclinical in vivo monitoring of new therapies and the clinical evaluation of CLL patients.

KW - Chronic Lymphocytic Leukemia (CLL)

KW - magnetic resonance imaging (MRI)

KW - ultra-small superparamagnetic iron oxide (USPIO)

KW - Xenograft NSG mice model

KW - disease monitoring

U2 - 10.1016/j.clim.2016.07.013

DO - 10.1016/j.clim.2016.07.013

M3 - Article

VL - 172

SP - 52

EP - 60

JO - Clinical Immunology

JF - Clinical Immunology

SN - 1521-6616

ER -