Abstract
Microarray technology has burgeoned over the past few years from nucleic acid-based arrays to tissue microarrays (TMAs). This study aimed to develop a technique to incorporate cell lines into an array and to demonstrate the usefulness of this technique by performing immunohistochemistry for beta-catenin. Cell suspensions were prepared from 23 tumor cell lines. These were fixed in formalin, suspended in agar, and embedded in paraffin to produce a cell block. A "tissue microarrayer" was used to remove triplicate, 0.6 mm-cores from each cell block and to transfer these into a recipient paraffin block at precise coordinates. Immunohistochemistry was used to identify cell lines positive for beta-catenin. Cultured cells were successfully incorporated into the microarray, with preservation of cell architecture and even distribution of cells within each core. A total of 18 of 69 cores (26%) were lost in processing. A total of 16 of 23 cell lines were identified as positive for membrane and cytoplasmic beta-catenin, and 6 of 23 were negative. Only one cell line was unscorable because of complete core loss. We have developed a "cell microarray" technique for analyzing antigen expression by immunohistochemistry in multiple cell lines in a single experiment. This novel application of microarrays permits high-throughput, cost-efficient analysis, with the potential to rapidly identify markers with potential diagnostic and therapeutic implications in human disease.
Original language | English |
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Pages (from-to) | 185-7 |
Number of pages | 3 |
Journal | In vitro cellular & developmental biology. Animal |
Volume | 41 |
Issue number | 7 |
DOIs | |
Publication status | Published - 15 Oct 2005 |
Externally published | Yes |
Keywords
- Biomarkers, Tumor
- Cell Line, Tumor
- Histological Techniques
- Humans
- Immunohistochemistry
- Molecular Diagnostic Techniques
- beta Catenin
- Journal Article
- Research Support, Non-U.S. Gov't