A novel cell array technique for high-throughput, cell-based analysis

A Waterworth, A Hanby, V Speirs

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Microarray technology has burgeoned over the past few years from nucleic acid-based arrays to tissue microarrays (TMAs). This study aimed to develop a technique to incorporate cell lines into an array and to demonstrate the usefulness of this technique by performing immunohistochemistry for beta-catenin. Cell suspensions were prepared from 23 tumor cell lines. These were fixed in formalin, suspended in agar, and embedded in paraffin to produce a cell block. A "tissue microarrayer" was used to remove triplicate, 0.6 mm-cores from each cell block and to transfer these into a recipient paraffin block at precise coordinates. Immunohistochemistry was used to identify cell lines positive for beta-catenin. Cultured cells were successfully incorporated into the microarray, with preservation of cell architecture and even distribution of cells within each core. A total of 18 of 69 cores (26%) were lost in processing. A total of 16 of 23 cell lines were identified as positive for membrane and cytoplasmic beta-catenin, and 6 of 23 were negative. Only one cell line was unscorable because of complete core loss. We have developed a "cell microarray" technique for analyzing antigen expression by immunohistochemistry in multiple cell lines in a single experiment. This novel application of microarrays permits high-throughput, cost-efficient analysis, with the potential to rapidly identify markers with potential diagnostic and therapeutic implications in human disease.

Original languageEnglish
Pages (from-to)185-7
Number of pages3
JournalIn vitro cellular & developmental biology. Animal
Volume41
Issue number7
DOIs
Publication statusPublished - 15 Oct 2005

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beta Catenin
Cell Line
Immunohistochemistry
Paraffin
Tumor Cell Line
Nucleic Acids
Formaldehyde
Agar
Cultured Cells
Suspensions
Cell Membrane
Technology
Antigens
Costs and Cost Analysis
Therapeutics

Keywords

  • Biomarkers, Tumor
  • Cell Line, Tumor
  • Histological Techniques
  • Humans
  • Immunohistochemistry
  • Molecular Diagnostic Techniques
  • beta Catenin
  • Journal Article
  • Research Support, Non-U.S. Gov't

Cite this

A novel cell array technique for high-throughput, cell-based analysis. / Waterworth, A; Hanby, A; Speirs, V.

In: In vitro cellular & developmental biology. Animal, Vol. 41, No. 7, 15.10.2005, p. 185-7.

Research output: Contribution to journalArticle

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