A NOVEL METHOD OF QUANTIFYING ROOT EXUDATION IN THE PRESENCE OF SOIL MICROFLORA

A A MEHARG, K KILLHAM

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Abstract

A microcosm is described in which root exudation may be estimated in the presence of microorganisms. Ryegrass seedlings are grown in microcosms in which roots were spatially separated from a microbial inoculant by a Millipore membrane. Seedlings grown in the microcosms were labelled with [C-14]-CO2, and the fate of the label within the plant and rhizosphere was determined. Inoculation of the microcosms with Cladosporium resinae increased net fixation of the [C-14] label compared to plants grown under sterile conditions. Inoculation also increased root exudation. The use of the microcosm was illustrated and its applications discussed.

Original languageEnglish
Pages (from-to)111-116
Number of pages6
JournalPlant and Soil
Volume133
Issue number1
Publication statusPublished - May 1991

Keywords

  • LOLIUM-PERENNE
  • RHIZOSPHERE
  • ROOT EXUDATION
  • BACTERIA
  • GROWTH
  • CARBON

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