Abstract
Selenium (Se) is an essential micronutrient for human health. The biological roles of the essential micronutrient Se are attributed to its presence in a range of 20-30 selenoproteins including the cytosolic and phospholipid hydroperoxide glutathione peroxidases (GPX1 and GPX4:). It has been suggested that GPX4, may play a role in regulation of leukotriene biosynthesis and thus inflammation. In eukaryotes Se is incorporated into selenoproteins as the amino acid selenocysteine in a process requiring a stem-loop within the 3' untranslated region (3'UTR) of the mRNA. In this study the region of the GPX4 gene corresponding to the 3'UTR was scanned for mutations in a group of 66 volunteers. The data show a T/C variant at position 718. The distribution of this SNP in our population was 34%' CC, 25% TT and 41% TC; i.e., it is in Hardy-Weinberg equilibrium. Individuals of different genotypes exhibited significant differences in the levels of lymphocyte 5-lipoxygenase total products, with C718 showing increased levels of those products compared to T718 and T/C718 (36% and 44% increases, respectively). The data suggest that the SNP718 that we have identified has functional effects and support the hypothesis that GPX4 plays a regulatory role in leukotriene biosynthesis. (C) 2002 Elsevier science (USA).
Original language | English |
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Pages (from-to) | 174-178 |
Number of pages | 5 |
Journal | Blood Cells, Molecules & Diseases |
Volume | 29 |
Issue number | 2 |
DOIs | |
Publication status | Published - Sept 2002 |
Keywords
- selenium
- phospholipid hydroperoxide glutathione peroxidase
- GPX4
- SNP
- lipoxygenase metabolism
- selenium deficiency
- gene-expression