A PARTIAL CHLOROFORM-FUMIGATION TECHNIQUE TO CHARACTERIZE THE SPATIAL LOCATION OF BACTERIA INTRODUCED INTO SOIL

Deborah Alison White, Alison Jane Wright, Lesley Anne Glover, James Ivor Prosser, John David Atkinson, Kenneth Stuart Killham

Research output: Contribution to journalArticle

Abstract

Bioluminescence-marked cells of Pseudomonas fluorescens were inoculated into soil by introduction into pores of two different size classes (<6 and 30-60 mum neck diameter). Partial chloroform fumigation resulted in a differential killing of cells depending on the placement of the inoculum within the soil pore network and on the period of fumigation. Reduced survival was associated with increasing periods (30 - 120 min) of fumigation, and with inoculum placement into larger rather than smaller pores. Comparison of the effects of partial fumigation on cells introduced into four soils of contrasting pore-size distribution highlighted the need to calibrate the method on the basis of air/water-filled pore space and chloroform diffusion dynamics for different soil types. It is proposed that partial fumigation facilitates spatial characterisation of the distribution of bacterial cells introduced into soils.

Original languageEnglish
Pages (from-to)191-195
Number of pages5
JournalBiology and Fertility of Soils
Volume17
Issue number3
Publication statusPublished - Mar 1994

Keywords

  • PSEUDOMONAS-FLUORESCENS
  • CHLOROFORM
  • FUMIGATION
  • SPATIAL LOCATION
  • PORE LOCATION
  • LEGUMINOSARUM BIOVAR TRIFOLII
  • POPULATION-DYNAMICS
  • MICROORGANISMS
  • DECOMPOSITION
  • MICROHABITATS
  • SURVIVAL
  • BIOMASS

Cite this

White, D. A., Wright, A. J., Glover, L. A., Prosser, J. I., Atkinson, J. D., & Killham, K. S. (1994). A PARTIAL CHLOROFORM-FUMIGATION TECHNIQUE TO CHARACTERIZE THE SPATIAL LOCATION OF BACTERIA INTRODUCED INTO SOIL. Biology and Fertility of Soils, 17(3), 191-195.

A PARTIAL CHLOROFORM-FUMIGATION TECHNIQUE TO CHARACTERIZE THE SPATIAL LOCATION OF BACTERIA INTRODUCED INTO SOIL. / White, Deborah Alison; Wright, Alison Jane; Glover, Lesley Anne; Prosser, James Ivor; Atkinson, John David; Killham, Kenneth Stuart.

In: Biology and Fertility of Soils, Vol. 17, No. 3, 03.1994, p. 191-195.

Research output: Contribution to journalArticle

White, Deborah Alison ; Wright, Alison Jane ; Glover, Lesley Anne ; Prosser, James Ivor ; Atkinson, John David ; Killham, Kenneth Stuart. / A PARTIAL CHLOROFORM-FUMIGATION TECHNIQUE TO CHARACTERIZE THE SPATIAL LOCATION OF BACTERIA INTRODUCED INTO SOIL. In: Biology and Fertility of Soils. 1994 ; Vol. 17, No. 3. pp. 191-195.
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abstract = "Bioluminescence-marked cells of Pseudomonas fluorescens were inoculated into soil by introduction into pores of two different size classes (<6 and 30-60 mum neck diameter). Partial chloroform fumigation resulted in a differential killing of cells depending on the placement of the inoculum within the soil pore network and on the period of fumigation. Reduced survival was associated with increasing periods (30 - 120 min) of fumigation, and with inoculum placement into larger rather than smaller pores. Comparison of the effects of partial fumigation on cells introduced into four soils of contrasting pore-size distribution highlighted the need to calibrate the method on the basis of air/water-filled pore space and chloroform diffusion dynamics for different soil types. It is proposed that partial fumigation facilitates spatial characterisation of the distribution of bacterial cells introduced into soils.",
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AB - Bioluminescence-marked cells of Pseudomonas fluorescens were inoculated into soil by introduction into pores of two different size classes (<6 and 30-60 mum neck diameter). Partial chloroform fumigation resulted in a differential killing of cells depending on the placement of the inoculum within the soil pore network and on the period of fumigation. Reduced survival was associated with increasing periods (30 - 120 min) of fumigation, and with inoculum placement into larger rather than smaller pores. Comparison of the effects of partial fumigation on cells introduced into four soils of contrasting pore-size distribution highlighted the need to calibrate the method on the basis of air/water-filled pore space and chloroform diffusion dynamics for different soil types. It is proposed that partial fumigation facilitates spatial characterisation of the distribution of bacterial cells introduced into soils.

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KW - FUMIGATION

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KW - PORE LOCATION

KW - LEGUMINOSARUM BIOVAR TRIFOLII

KW - POPULATION-DYNAMICS

KW - MICROORGANISMS

KW - DECOMPOSITION

KW - MICROHABITATS

KW - SURVIVAL

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