A protective role for nitric oxide and salicylic acid for arsenite phytotoxicity in rice (Oryza sativa L.)

Amit Pal Singh; Garima Dixit; Amit Kumar; Seema Mishra; Navin Kumar; Sameer Dixit; Pradyumna Kumar Singh; Sanjay Dwivedi; Prabodh Kumar Trivedi; Vivek Pandey; Om Prakash Dhankher; Gareth J. Norton; Debasis Chakrabarty; Rudra Deo Tripathi

doi:10.1016/j.plaphy.2017.02.019

A protective role for nitric oxide and salicylic acid for arsenite phytotoxicity in rice (Oryza sativa L.)

Amit Pal Singh, Garima Dixit, Amit Kumar, Seema Mishra, Navin Kumar, Sameer Dixit, Pradyumna Kumar Singh, Sanjay Dwivedi, Prabodh Kumar Trivedi, Vivek Pandey, Om Prakash Dhankher, Gareth J. Norton, Debasis Chakrabarty, Rudra Deo Tripathi^*

^*Corresponding author for this work

Aberdeen Centre For Environmental Sustainability

Research output: Contribution to journal › Article › peer-review

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Abstract

Nitric oxide (NO) and salicylic acid (SA) are important signaling molecules in plant system. In the present study both NO and SA showed a protective role against arsenite (As^III) stress in rice plants when supplied exogenously. The application of NO and SA alleviated the negative impact of As^III on plant growth. Nitric oxide supplementation to As^III treated plants greatly decreased arsenic (As) accumulation in the roots as well as shoots/roots translocation factor. Arsenite exposure in plants decreased the endogenous levels of NO and SA. Exogenous supplementation of SA not only enhanced endogenous level of SA but also the level of NO through enhanced nitrate reductase (NR) activity, whether As^III was present or not. Exogenously supplied NO decreased the NR activity and level of endogenous NO. Arsenic accumulation was positively correlated with the expression level of OsLsi1, a transporter responsible for As^III uptake. The endogenous level of NO and SA were positively correlated to each other either when As^III was present or not. This close relationship indicates that NO and SA work in harmony to modulate the signaling response in As^III stressed plants.

Original language	English
Pages (from-to)	163-173
Number of pages	11
Journal	Plant Physiology and Biochemistry
Volume	115
Early online date	24 Feb 2017
DOIs	https://doi.org/10.1016/j.plaphy.2017.02.019
Publication status	Published - 1 Jun 2017

Keywords

Arsenic
Nitric oxide
Rice
Salicylic acid
Transporters

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Singh, A. P., Dixit, G., Kumar, A., Mishra, S., Kumar, N., Dixit, S., Singh, P. K., Dwivedi, S., Trivedi, P. K., Pandey, V., Dhankher, O. P., Norton, G. J., Chakrabarty, D., & Tripathi, R. D. (2017). A protective role for nitric oxide and salicylic acid for arsenite phytotoxicity in rice (Oryza sativa L.). Plant Physiology and Biochemistry, 115, 163-173. https://doi.org/10.1016/j.plaphy.2017.02.019

Singh, AP, Dixit, G, Kumar, A, Mishra, S, Kumar, N, Dixit, S, Singh, PK, Dwivedi, S, Trivedi, PK, Pandey, V, Dhankher, OP, Norton, GJ, Chakrabarty, D & Tripathi, RD 2017, 'A protective role for nitric oxide and salicylic acid for arsenite phytotoxicity in rice (Oryza sativa L.)', Plant Physiology and Biochemistry, vol. 115, pp. 163-173. https://doi.org/10.1016/j.plaphy.2017.02.019

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title = "A protective role for nitric oxide and salicylic acid for arsenite phytotoxicity in rice (Oryza sativa L.)",

abstract = "Nitric oxide (NO) and salicylic acid (SA) are important signaling molecules in plant system. In the present study both NO and SA showed a protective role against arsenite (AsIII) stress in rice plants when supplied exogenously. The application of NO and SA alleviated the negative impact of AsIII on plant growth. Nitric oxide supplementation to AsIII treated plants greatly decreased arsenic (As) accumulation in the roots as well as shoots/roots translocation factor. Arsenite exposure in plants decreased the endogenous levels of NO and SA. Exogenous supplementation of SA not only enhanced endogenous level of SA but also the level of NO through enhanced nitrate reductase (NR) activity, whether AsIII was present or not. Exogenously supplied NO decreased the NR activity and level of endogenous NO. Arsenic accumulation was positively correlated with the expression level of OsLsi1, a transporter responsible for AsIII uptake. The endogenous level of NO and SA were positively correlated to each other either when AsIII was present or not. This close relationship indicates that NO and SA work in harmony to modulate the signaling response in AsIII stressed plants.",

keywords = "Arsenic, Nitric oxide, Rice, Salicylic acid, Transporters",

author = "Singh, {Amit Pal} and Garima Dixit and Amit Kumar and Seema Mishra and Navin Kumar and Sameer Dixit and Singh, {Pradyumna Kumar} and Sanjay Dwivedi and Trivedi, {Prabodh Kumar} and Vivek Pandey and Dhankher, {Om Prakash} and Norton, {Gareth J.} and Debasis Chakrabarty and Tripathi, {Rudra Deo}",

note = "The authors are thankful to Director, CSIR-National Botanical Research Institute (CSIR-NBRI), Lucknow for the facilities and for the financial support from the network projects (CSIR-INDEPTH), New Delhi, India. APS is thankful to CSIR New Delhi, India respectively, for the award of Research Associateship. RDT is gratefully thankful to Award of Emeritus Scientist (CSIR). GD is thankful to SERB-DST, New Delhi for award of NPDF. AK is thankful to UGC for award of DSKPDF. Award of Fast Track Scientist to SM from DST is gratefully acknowledged. We are also thankful to Mr. Dilip Chakraborty for technical assistance.",

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month = jun,

day = "1",

doi = "10.1016/j.plaphy.2017.02.019",

language = "English",

volume = "115",

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T1 - A protective role for nitric oxide and salicylic acid for arsenite phytotoxicity in rice (Oryza sativa L.)

AU - Singh, Amit Pal

AU - Dixit, Garima

AU - Kumar, Amit

AU - Mishra, Seema

AU - Kumar, Navin

AU - Dixit, Sameer

AU - Singh, Pradyumna Kumar

AU - Dwivedi, Sanjay

AU - Trivedi, Prabodh Kumar

AU - Pandey, Vivek

AU - Dhankher, Om Prakash

AU - Norton, Gareth J.

AU - Chakrabarty, Debasis

AU - Tripathi, Rudra Deo

N1 - The authors are thankful to Director, CSIR-National Botanical Research Institute (CSIR-NBRI), Lucknow for the facilities and for the financial support from the network projects (CSIR-INDEPTH), New Delhi, India. APS is thankful to CSIR New Delhi, India respectively, for the award of Research Associateship. RDT is gratefully thankful to Award of Emeritus Scientist (CSIR). GD is thankful to SERB-DST, New Delhi for award of NPDF. AK is thankful to UGC for award of DSKPDF. Award of Fast Track Scientist to SM from DST is gratefully acknowledged. We are also thankful to Mr. Dilip Chakraborty for technical assistance.

PY - 2017/6/1

Y1 - 2017/6/1

N2 - Nitric oxide (NO) and salicylic acid (SA) are important signaling molecules in plant system. In the present study both NO and SA showed a protective role against arsenite (AsIII) stress in rice plants when supplied exogenously. The application of NO and SA alleviated the negative impact of AsIII on plant growth. Nitric oxide supplementation to AsIII treated plants greatly decreased arsenic (As) accumulation in the roots as well as shoots/roots translocation factor. Arsenite exposure in plants decreased the endogenous levels of NO and SA. Exogenous supplementation of SA not only enhanced endogenous level of SA but also the level of NO through enhanced nitrate reductase (NR) activity, whether AsIII was present or not. Exogenously supplied NO decreased the NR activity and level of endogenous NO. Arsenic accumulation was positively correlated with the expression level of OsLsi1, a transporter responsible for AsIII uptake. The endogenous level of NO and SA were positively correlated to each other either when AsIII was present or not. This close relationship indicates that NO and SA work in harmony to modulate the signaling response in AsIII stressed plants.

AB - Nitric oxide (NO) and salicylic acid (SA) are important signaling molecules in plant system. In the present study both NO and SA showed a protective role against arsenite (AsIII) stress in rice plants when supplied exogenously. The application of NO and SA alleviated the negative impact of AsIII on plant growth. Nitric oxide supplementation to AsIII treated plants greatly decreased arsenic (As) accumulation in the roots as well as shoots/roots translocation factor. Arsenite exposure in plants decreased the endogenous levels of NO and SA. Exogenous supplementation of SA not only enhanced endogenous level of SA but also the level of NO through enhanced nitrate reductase (NR) activity, whether AsIII was present or not. Exogenously supplied NO decreased the NR activity and level of endogenous NO. Arsenic accumulation was positively correlated with the expression level of OsLsi1, a transporter responsible for AsIII uptake. The endogenous level of NO and SA were positively correlated to each other either when AsIII was present or not. This close relationship indicates that NO and SA work in harmony to modulate the signaling response in AsIII stressed plants.

KW - Arsenic

KW - Nitric oxide

KW - Rice

KW - Salicylic acid

KW - Transporters

UR - http://www.scopus.com/inward/record.url?scp=85016427646&partnerID=8YFLogxK

U2 - 10.1016/j.plaphy.2017.02.019

DO - 10.1016/j.plaphy.2017.02.019

M3 - Article

AN - SCOPUS:85016427646

VL - 115

SP - 163

EP - 173

JO - Plant Physiology and Biochemistry

JF - Plant Physiology and Biochemistry

SN - 0981-9428

ER -

A protective role for nitric oxide and salicylic acid for arsenite phytotoxicity in rice (Oryza sativa L.)

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