A protective role for nitric oxide and salicylic acid for arsenite phytotoxicity in rice (Oryza sativa L.)

Amit Pal Singh, Garima Dixit, Amit Kumar, Seema Mishra, Navin Kumar, Sameer Dixit, Pradyumna Kumar Singh, Sanjay Dwivedi, Prabodh Kumar Trivedi, Vivek Pandey, Om Prakash Dhankher, Gareth J. Norton, Debasis Chakrabarty, Rudra Deo Tripathi*

*Corresponding author for this work

Research output: Contribution to journalArticle

24 Citations (Scopus)
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Abstract

Nitric oxide (NO) and salicylic acid (SA) are important signaling molecules in plant system. In the present study both NO and SA showed a protective role against arsenite (AsIII) stress in rice plants when supplied exogenously. The application of NO and SA alleviated the negative impact of AsIII on plant growth. Nitric oxide supplementation to AsIII treated plants greatly decreased arsenic (As) accumulation in the roots as well as shoots/roots translocation factor. Arsenite exposure in plants decreased the endogenous levels of NO and SA. Exogenous supplementation of SA not only enhanced endogenous level of SA but also the level of NO through enhanced nitrate reductase (NR) activity, whether AsIII was present or not. Exogenously supplied NO decreased the NR activity and level of endogenous NO. Arsenic accumulation was positively correlated with the expression level of OsLsi1, a transporter responsible for AsIII uptake. The endogenous level of NO and SA were positively correlated to each other either when AsIII was present or not. This close relationship indicates that NO and SA work in harmony to modulate the signaling response in AsIII stressed plants.

Original languageEnglish
Pages (from-to)163-173
Number of pages11
JournalPlant Physiology and Biochemistry
Volume115
Early online date24 Feb 2017
DOIs
Publication statusPublished - 1 Jun 2017

Fingerprint

arsenites
Salicylic Acid
phytotoxicity
salicylic acid
nitric oxide
Oryza sativa
Nitric Oxide
rice
Nitrate Reductase
Arsenic
nitrate reductase
arsenic
Oryza
arsenite
transporters
plant growth
uptake mechanisms

Keywords

  • Arsenic
  • Nitric oxide
  • Rice
  • Salicylic acid
  • Transporters

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

Cite this

A protective role for nitric oxide and salicylic acid for arsenite phytotoxicity in rice (Oryza sativa L.). / Singh, Amit Pal; Dixit, Garima; Kumar, Amit; Mishra, Seema; Kumar, Navin; Dixit, Sameer; Singh, Pradyumna Kumar; Dwivedi, Sanjay; Trivedi, Prabodh Kumar; Pandey, Vivek; Dhankher, Om Prakash; Norton, Gareth J.; Chakrabarty, Debasis; Tripathi, Rudra Deo.

In: Plant Physiology and Biochemistry, Vol. 115, 01.06.2017, p. 163-173.

Research output: Contribution to journalArticle

Singh, AP, Dixit, G, Kumar, A, Mishra, S, Kumar, N, Dixit, S, Singh, PK, Dwivedi, S, Trivedi, PK, Pandey, V, Dhankher, OP, Norton, GJ, Chakrabarty, D & Tripathi, RD 2017, 'A protective role for nitric oxide and salicylic acid for arsenite phytotoxicity in rice (Oryza sativa L.)', Plant Physiology and Biochemistry, vol. 115, pp. 163-173. https://doi.org/10.1016/j.plaphy.2017.02.019
Singh, Amit Pal ; Dixit, Garima ; Kumar, Amit ; Mishra, Seema ; Kumar, Navin ; Dixit, Sameer ; Singh, Pradyumna Kumar ; Dwivedi, Sanjay ; Trivedi, Prabodh Kumar ; Pandey, Vivek ; Dhankher, Om Prakash ; Norton, Gareth J. ; Chakrabarty, Debasis ; Tripathi, Rudra Deo. / A protective role for nitric oxide and salicylic acid for arsenite phytotoxicity in rice (Oryza sativa L.). In: Plant Physiology and Biochemistry. 2017 ; Vol. 115. pp. 163-173.
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abstract = "Nitric oxide (NO) and salicylic acid (SA) are important signaling molecules in plant system. In the present study both NO and SA showed a protective role against arsenite (AsIII) stress in rice plants when supplied exogenously. The application of NO and SA alleviated the negative impact of AsIII on plant growth. Nitric oxide supplementation to AsIII treated plants greatly decreased arsenic (As) accumulation in the roots as well as shoots/roots translocation factor. Arsenite exposure in plants decreased the endogenous levels of NO and SA. Exogenous supplementation of SA not only enhanced endogenous level of SA but also the level of NO through enhanced nitrate reductase (NR) activity, whether AsIII was present or not. Exogenously supplied NO decreased the NR activity and level of endogenous NO. Arsenic accumulation was positively correlated with the expression level of OsLsi1, a transporter responsible for AsIII uptake. The endogenous level of NO and SA were positively correlated to each other either when AsIII was present or not. This close relationship indicates that NO and SA work in harmony to modulate the signaling response in AsIII stressed plants.",
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AU - Kumar, Navin

AU - Dixit, Sameer

AU - Singh, Pradyumna Kumar

AU - Dwivedi, Sanjay

AU - Trivedi, Prabodh Kumar

AU - Pandey, Vivek

AU - Dhankher, Om Prakash

AU - Norton, Gareth J.

AU - Chakrabarty, Debasis

AU - Tripathi, Rudra Deo

N1 - The authors are thankful to Director, CSIR-National Botanical Research Institute (CSIR-NBRI), Lucknow for the facilities and for the financial support from the network projects (CSIR-INDEPTH), New Delhi, India. APS is thankful to CSIR New Delhi, India respectively, for the award of Research Associateship. RDT is gratefully thankful to Award of Emeritus Scientist (CSIR). GD is thankful to SERB-DST, New Delhi for award of NPDF. AK is thankful to UGC for award of DSKPDF. Award of Fast Track Scientist to SM from DST is gratefully acknowledged. We are also thankful to Mr. Dilip Chakraborty for technical assistance.

PY - 2017/6/1

Y1 - 2017/6/1

N2 - Nitric oxide (NO) and salicylic acid (SA) are important signaling molecules in plant system. In the present study both NO and SA showed a protective role against arsenite (AsIII) stress in rice plants when supplied exogenously. The application of NO and SA alleviated the negative impact of AsIII on plant growth. Nitric oxide supplementation to AsIII treated plants greatly decreased arsenic (As) accumulation in the roots as well as shoots/roots translocation factor. Arsenite exposure in plants decreased the endogenous levels of NO and SA. Exogenous supplementation of SA not only enhanced endogenous level of SA but also the level of NO through enhanced nitrate reductase (NR) activity, whether AsIII was present or not. Exogenously supplied NO decreased the NR activity and level of endogenous NO. Arsenic accumulation was positively correlated with the expression level of OsLsi1, a transporter responsible for AsIII uptake. The endogenous level of NO and SA were positively correlated to each other either when AsIII was present or not. This close relationship indicates that NO and SA work in harmony to modulate the signaling response in AsIII stressed plants.

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