A proteomics approach to identify changes in protein profiles in pre-cancerous colon

Janice Drew, Garry J Rucklidge, Gary Duncan, Awni Lufty, Andrew J Farquharson, Martin D Reid, Wendy R Russell, Philip C Morrice, John R Arthur, Garry G Duthie

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The development of colon cancer is characterised by alterations in multiple genetic and epigenetic pathways in colon tissue leading ultimately to deregulation of colon epithelial cells. Early detection is an important factor in decreasing colon cancer deaths. Proteomic techniques were used to identify potential early markers in colon tissue exhibiting pre-cancerous activity that may characterise pathological changes in a chemically induced colon cancer rat model. Protein profiles were assessed in soluble and insoluble fractions prepared from distal colon of rats treated with the colonotropic carcinogen, dimethylhydrazine. Alterations in protein profiles were associated with the presence of aberrant crypt foci, hyperplasia and dysplasia, microanatomical changes, and metabolic changes in rat colon. These changes may have a potential role in the identification of pre-pathological features preceding colon tumorigenesis. (c) 2005 Elsevier Inc. All rights reserved.

Original languageEnglish
Pages (from-to)81-87
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume330
Issue number1
DOIs
Publication statusPublished - 29 Apr 2005

Keywords

  • proteomics
  • colon cancer
  • rat
  • aberrant crypt foci
  • prostaglandin
  • collagenous colitis
  • oxidative stress
  • gene expression
  • carbonyl reductase
  • colorectal cancer
  • cells
  • diet
  • carcinogenesis
  • azoxymethane

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