Abstract
The asexual multinucleated sporangia of Phytophthora infestans can germinate directly through a germ tube or indirectly by releasing zoospores. The molecular mechanisms controlling sporangial cytokinesis or sporangial cleavage, and zoospore release are largely unknown. Sporangial cleavage is initiated by a cold shock that eventually compartmentalizes single nuclei within each zoospore. Comparison of EST representation in different cDNA libraries revealed a putative ATP-dependent DEAD-box RNA-helicase gene in P. infestans, Pi-RNH1, which has a 140-fold increased expression level in young zoospores compared to uncleaved sporangia. RNA interference was employed to determine the role of Pi-RNH1 in zoospore development. Silencing efficiencies of up to 99% were achieved in some transiently-silenced lines. These Pi-RNH1-silenced lines produced large aberrant zoospores that had undergone partial cleavage and often had multiple flagella on their surface. Transmission electron microscopy revealed that cytoplasmic vesicles fused in the silenced lines, resulting in the formation of large vesicles. The Pi-RNH1-silenced zoospores were also sensitive to osmotic pressure and often ruptured upon release from the sporangia. These findings indicate that Pi-RNH1 has a major function in zoospore development and its potential role in cytokinesis is discussed. (c) 2008 Elsevier Inc. All rights reserved.
Original language | English |
---|---|
Pages (from-to) | 954-962 |
Number of pages | 9 |
Journal | Fungal Genetics and Biology |
Volume | 45 |
Issue number | 6 |
Early online date | 20 Mar 2008 |
DOIs | |
Publication status | Published - Jun 2008 |
Bibliographical note
A paid open access option is available for this journal.Voluntary deposit by author of pre-print allowed on Institutions open scholarly website and pre-print servers
Voluntary deposit by author of authors post-print allowed on institutions open scholarly website including Institutional Repository
Deposit due to Funding Body, Institutional and Governmental mandate only allowed where seperate agreement between repository and publisher exists
Set statement to accompany deposit
Published source must be acknowledged
Must link to journal home page or articles' DOI
Publisher's version/PDF cannot be used
Articles in some journals can be made Open Access on payment of additional charge
NIH Authors articles will be submitted to PMC after 12 months
Authors who are required to deposit in subject repositories may also use Sponsorship Option
Pre-prints for 'The Lancet' cannot be archived
Keywords
- Phytophthora infestans
- oomycete
- RNA-helicase
- zoospore
- pathogen
- development
- protein-synthesis
- sporangial cleavage
- potato infection
- gene
- germination
- palmivora
- calcium
- transcription
- cytokines
- activation
Fingerprint
Dive into the research topics of 'A putative DEAD-box RNA-helicase is required for normal zoospore development in the late blight pathogen Phytophthora infestans'. Together they form a unique fingerprint.Equipment
-
Microscopy and Histology
Debbie Wilkinson (Manager) & Gillian Milne (Manager)
Medical SciencesResearch Facilities: Facility