Abstract
Unlike other Salmonellae, the intracellular bacterial human pathogen Salmonella Typhi exhibits strict host specificity. The molecular bases for this restriction are unknown. Here we found that the expression of a single type III secretion system effector protein from broad-host Salmonella Typhimurium allowed Salmonella Typhi to survive and replicate within macrophages and tissues from mice, a nonpermissive host. This effector proteolytically targeted Rab32, which controls traffic to lysosome-related organelles in conjunction with components of the biogenesis of lysosome-related organelle complexes (BLOCs). RNA interference-mediated depletion of Rab32 or of an essential component of a BLOC complex was sufficient to allow S. Typhi to survive within mouse macrophages. Furthermore, S. Typhi was able to survive in macrophages from mice defective in BLOC components.
Original language | English |
---|---|
Pages (from-to) | 960-963 |
Number of pages | 4 |
Journal | Science |
Volume | 338 |
Issue number | 6109 |
DOIs | |
Publication status | Published - 16 Nov 2012 |
Keywords
- amino acid sequence
- animals
- bacterial secretion systems
- COS cells
- cercopithecus aethiops
- host-pathogen interactions
- humans
- lysosomes
- mice
- mice, inbred C57BL
- molecular sequence data
- phylogeny
- RNA interference
- Salmonella typhi
- rab GTP-binding proteins