A reliable RT-PCR-ELISA method for the detection of infectious pancreatic necrosis virus (IPNV) in farmed rainbow trout

S A Milne, S Gallacher, P Cash, A J R Porter

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

A new method, termed RT-PCR-ELISA, was evaluated for ease of use, reliability and sensitivity when detecting infectious pancreatic necrosis virus (IPNV) present in trout kidney tissue. The method had comparable sensitivity to existing PCR assays and could successfully detect 1.5 x 10(4) pfu IPNV in artificially contaminated trout kidney samples. The technique was easily established in a new laboratory and required no specialised equipment. The method had a high sample throughput capable of screening 96 samples per run, making the technique extremely time efficient. The RT-PCR-ELISA is a safe, quick, reliable technique, which has the potential for use as a standard virus detection method. (c) 2005 Elsevier B.V. All rights reserved.

Original languageEnglish
Pages (from-to)92-96
Number of pages5
JournalJournal of Virological Methods
Volume132
Issue number1-2
DOIs
Publication statusPublished - Mar 2006

Keywords

  • IPNV
  • detection
  • RT-PCR
  • ELISA
  • reverse-transcriptase PCR
  • hepatitis-A virus
  • ennteric viruses
  • assay

Cite this