A representative of the dominant human colonic Firmicutes, Roseburia faecis M72/1, forms a novel bacteriocin-like substance

Diane Hatziioanou; Melinda J. Mayer; Sylvia H. Duncan; Harry J. Flint; Arjan Narbad

doi:10.1016/j.anaerobe.2013.07.006

A representative of the dominant human colonic Firmicutes, Roseburia faecis M72/1, forms a novel bacteriocin-like substance

Diane Hatziioanou, Melinda J. Mayer^*, Sylvia H. Duncan, Harry J. Flint, Arjan Narbad

^*Corresponding author for this work

The Rowett Institute of Nutrition and Health

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

During screening of human gut isolates in search of novel antimicrobials, the butyrate-producing strain Roseburia faecis M72/1 was found to produce an inhibitory substance active against Bacillus subtilis. Partial purification of the antimicrobial was achieved and activity found to be heat labile. Our findings suggest that R. faecis M72/1 produces a proteinaceous inhibitor whose production may be triggered by trypsin in the gastrointestinal tract. (c) 2013 Elsevier Ltd. All rights reserved.

Original language	English
Pages (from-to)	5-8
Number of pages	4
Journal	Anaerobe
Volume	23
Early online date	31 Jul 2013
DOIs	https://doi.org/10.1016/j.anaerobe.2013.07.006
Publication status	Published - Oct 2013

Keywords

roseburia faecis
bacteriocin-like inhibitory substance (BLIS)
firmicutes anaerobe
gut microbiota
trypsin
ruminococcus-gnavus strain
lactic-acid bacteria
human feces
purification
microbiota
probiotics
diversity
butyrate
health
gene

Access to Document

10.1016/j.anaerobe.2013.07.006

Cite this

@article{a2dfbe81b5b545059ac22531f67b1b83,

title = "A representative of the dominant human colonic Firmicutes, Roseburia faecis M72/1, forms a novel bacteriocin-like substance",

abstract = "During screening of human gut isolates in search of novel antimicrobials, the butyrate-producing strain Roseburia faecis M72/1 was found to produce an inhibitory substance active against Bacillus subtilis. Partial purification of the antimicrobial was achieved and activity found to be heat labile. Our findings suggest that R. faecis M72/1 produces a proteinaceous inhibitor whose production may be triggered by trypsin in the gastrointestinal tract. (c) 2013 Elsevier Ltd. All rights reserved.",

keywords = "roseburia faecis, bacteriocin-like inhibitory substance (BLIS), firmicutes anaerobe, gut microbiota, trypsin, ruminococcus-gnavus strain, lactic-acid bacteria, human feces, purification, microbiota, probiotics, diversity, butyrate, health, gene",

author = "Diane Hatziioanou and Mayer, {Melinda J.} and Duncan, {Sylvia H.} and Flint, {Harry J.} and Arjan Narbad",

note = "This work was supported by a Biotechnology and Biological Sciences Research Council studentship grant to DH. SHD and HJF acknowledge support from the Scottish Government RESAS programme.",

year = "2013",

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doi = "10.1016/j.anaerobe.2013.07.006",

language = "English",

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T1 - A representative of the dominant human colonic Firmicutes, Roseburia faecis M72/1, forms a novel bacteriocin-like substance

AU - Hatziioanou, Diane

AU - Mayer, Melinda J.

AU - Duncan, Sylvia H.

AU - Flint, Harry J.

AU - Narbad, Arjan

N1 - This work was supported by a Biotechnology and Biological Sciences Research Council studentship grant to DH. SHD and HJF acknowledge support from the Scottish Government RESAS programme.

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AB - During screening of human gut isolates in search of novel antimicrobials, the butyrate-producing strain Roseburia faecis M72/1 was found to produce an inhibitory substance active against Bacillus subtilis. Partial purification of the antimicrobial was achieved and activity found to be heat labile. Our findings suggest that R. faecis M72/1 produces a proteinaceous inhibitor whose production may be triggered by trypsin in the gastrointestinal tract. (c) 2013 Elsevier Ltd. All rights reserved.

KW - roseburia faecis

KW - bacteriocin-like inhibitory substance (BLIS)

KW - firmicutes anaerobe

KW - gut microbiota

KW - trypsin

KW - ruminococcus-gnavus strain

KW - lactic-acid bacteria

KW - human feces

KW - purification

KW - microbiota

KW - probiotics

KW - diversity

KW - butyrate

KW - health

KW - gene

U2 - 10.1016/j.anaerobe.2013.07.006

DO - 10.1016/j.anaerobe.2013.07.006

M3 - Article

VL - 23

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EP - 8

JO - Anaerobe

JF - Anaerobe

SN - 1075-9964

ER -