A role for mechanosensitive channels in survival of stationary phase

regulation of channel expression by RpoS

Neil R Stokes, Heath D Murray, Chandrasekaran Subramaniam, Richard L Gourse, Petra Louis, Wendy Bartlett, Samantha Miller, Ian R Booth

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

The mechanosensitive (MS) channels MscS and MscL are essential for the survival of hypoosmotic shock by Escherichia coli cells. We demonstrate that MscS and MscL are induced by osmotic stress and by entry into stationary phase. Reduced levels of MS proteins and reduced expression of mscL- and mscS-LacZ fusions in an rpoS mutant strain suggested that the RNA polymerase holoenzyme containing sigmaS is responsible, at least in part, for regulating production of MS channel proteins. Consistent with the model that the effect of sigmaS is direct, the MscS and MscL promoters both use RNA polymerase containing sigmaS in vitro. Conversely, clpP or rssB mutations, which cause enhanced levels of sigmaS, show increased MS channel protein synthesis. RpoS null mutants are sensitive to hypoosmotic shock upon entry into stationary phase. These data suggest that MscS and MscL are components of the RpoS regulon and play an important role in ensuring structural integrity in stationary phase bacteria.
Original languageEnglish
Pages (from-to)15959-15964
Number of pages6
JournalPNAS
Volume100
Issue number26
Early online date11 Dec 2003
DOIs
Publication statusPublished - 23 Dec 2003

Keywords

  • Bacterial Proteins
  • Base Sequence
  • DNA Primers
  • Escherichia coli
  • Escherichia coli Proteins
  • Gene Expression Regulation, Bacterial
  • Genes, Reporter
  • Ion Channels
  • Mechanotransduction, Cellular
  • Plasmids
  • Sigma Factor
  • Transcription, Genetic
  • beta-Galactosidase

Cite this

A role for mechanosensitive channels in survival of stationary phase : regulation of channel expression by RpoS. / Stokes, Neil R; Murray, Heath D; Subramaniam, Chandrasekaran; Gourse, Richard L; Louis, Petra; Bartlett, Wendy; Miller, Samantha; Booth, Ian R.

In: PNAS, Vol. 100, No. 26, 23.12.2003, p. 15959-15964.

Research output: Contribution to journalArticle

Stokes, Neil R ; Murray, Heath D ; Subramaniam, Chandrasekaran ; Gourse, Richard L ; Louis, Petra ; Bartlett, Wendy ; Miller, Samantha ; Booth, Ian R. / A role for mechanosensitive channels in survival of stationary phase : regulation of channel expression by RpoS. In: PNAS. 2003 ; Vol. 100, No. 26. pp. 15959-15964.
@article{c9813760882f494daa07b419b64cbf23,
title = "A role for mechanosensitive channels in survival of stationary phase: regulation of channel expression by RpoS",
abstract = "The mechanosensitive (MS) channels MscS and MscL are essential for the survival of hypoosmotic shock by Escherichia coli cells. We demonstrate that MscS and MscL are induced by osmotic stress and by entry into stationary phase. Reduced levels of MS proteins and reduced expression of mscL- and mscS-LacZ fusions in an rpoS mutant strain suggested that the RNA polymerase holoenzyme containing sigmaS is responsible, at least in part, for regulating production of MS channel proteins. Consistent with the model that the effect of sigmaS is direct, the MscS and MscL promoters both use RNA polymerase containing sigmaS in vitro. Conversely, clpP or rssB mutations, which cause enhanced levels of sigmaS, show increased MS channel protein synthesis. RpoS null mutants are sensitive to hypoosmotic shock upon entry into stationary phase. These data suggest that MscS and MscL are components of the RpoS regulon and play an important role in ensuring structural integrity in stationary phase bacteria.",
keywords = "Bacterial Proteins, Base Sequence, DNA Primers, Escherichia coli, Escherichia coli Proteins, Gene Expression Regulation, Bacterial, Genes, Reporter, Ion Channels, Mechanotransduction, Cellular, Plasmids, Sigma Factor, Transcription, Genetic, beta-Galactosidase",
author = "Stokes, {Neil R} and Murray, {Heath D} and Chandrasekaran Subramaniam and Gourse, {Richard L} and Petra Louis and Wendy Bartlett and Samantha Miller and Booth, {Ian R}",
year = "2003",
month = "12",
day = "23",
doi = "10.1073/pnas.2536607100",
language = "English",
volume = "100",
pages = "15959--15964",
journal = "PNAS",
issn = "0027-8424",
publisher = "NATL ACAD SCIENCES",
number = "26",

}

TY - JOUR

T1 - A role for mechanosensitive channels in survival of stationary phase

T2 - regulation of channel expression by RpoS

AU - Stokes, Neil R

AU - Murray, Heath D

AU - Subramaniam, Chandrasekaran

AU - Gourse, Richard L

AU - Louis, Petra

AU - Bartlett, Wendy

AU - Miller, Samantha

AU - Booth, Ian R

PY - 2003/12/23

Y1 - 2003/12/23

N2 - The mechanosensitive (MS) channels MscS and MscL are essential for the survival of hypoosmotic shock by Escherichia coli cells. We demonstrate that MscS and MscL are induced by osmotic stress and by entry into stationary phase. Reduced levels of MS proteins and reduced expression of mscL- and mscS-LacZ fusions in an rpoS mutant strain suggested that the RNA polymerase holoenzyme containing sigmaS is responsible, at least in part, for regulating production of MS channel proteins. Consistent with the model that the effect of sigmaS is direct, the MscS and MscL promoters both use RNA polymerase containing sigmaS in vitro. Conversely, clpP or rssB mutations, which cause enhanced levels of sigmaS, show increased MS channel protein synthesis. RpoS null mutants are sensitive to hypoosmotic shock upon entry into stationary phase. These data suggest that MscS and MscL are components of the RpoS regulon and play an important role in ensuring structural integrity in stationary phase bacteria.

AB - The mechanosensitive (MS) channels MscS and MscL are essential for the survival of hypoosmotic shock by Escherichia coli cells. We demonstrate that MscS and MscL are induced by osmotic stress and by entry into stationary phase. Reduced levels of MS proteins and reduced expression of mscL- and mscS-LacZ fusions in an rpoS mutant strain suggested that the RNA polymerase holoenzyme containing sigmaS is responsible, at least in part, for regulating production of MS channel proteins. Consistent with the model that the effect of sigmaS is direct, the MscS and MscL promoters both use RNA polymerase containing sigmaS in vitro. Conversely, clpP or rssB mutations, which cause enhanced levels of sigmaS, show increased MS channel protein synthesis. RpoS null mutants are sensitive to hypoosmotic shock upon entry into stationary phase. These data suggest that MscS and MscL are components of the RpoS regulon and play an important role in ensuring structural integrity in stationary phase bacteria.

KW - Bacterial Proteins

KW - Base Sequence

KW - DNA Primers

KW - Escherichia coli

KW - Escherichia coli Proteins

KW - Gene Expression Regulation, Bacterial

KW - Genes, Reporter

KW - Ion Channels

KW - Mechanotransduction, Cellular

KW - Plasmids

KW - Sigma Factor

KW - Transcription, Genetic

KW - beta-Galactosidase

U2 - 10.1073/pnas.2536607100

DO - 10.1073/pnas.2536607100

M3 - Article

VL - 100

SP - 15959

EP - 15964

JO - PNAS

JF - PNAS

SN - 0027-8424

IS - 26

ER -