Abstract
Original language | English |
---|---|
Pages (from-to) | 27-39 |
Number of pages | 13 |
Journal | Mechanisms of Development |
Volume | 93 |
Issue number | 1-2 |
Early online date | 20 Apr 2000 |
DOIs | |
Publication status | Published - 1 May 2000 |
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Keywords
- Amino Acid Sequence
- Animals
- Base Sequence
- Cattle
- DNA, Complementary
- DNA-Binding Proteins
- Dexamethasone
- Early Growth Response Protein 1
- Gastrula
- Gene Expression Regulation, Developmental
- Genes, Tumor Suppressor
- Glycoproteins
- Homeodomain Proteins
- Humans
- Immediate-Early Proteins
- Mesoderm
- Molecular Sequence Data
- Neoplasm Proteins
- Recombinant Fusion Proteins
- T-Box Domain Proteins
- Trans-Activators
- Transcription Factors
- Wnt Proteins
- Xenopus
- Xenopus Proteins
- Brachyury
- Xbra
- Pintallavis
- Xenopus laevis
Cite this
A screen for targets of the Xenopus T-box gene Xbra. / Saka, Yasushi; Tada, M; Smith, J C.
In: Mechanisms of Development, Vol. 93, No. 1-2, 01.05.2000, p. 27-39.Research output: Contribution to journal › Article
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TY - JOUR
T1 - A screen for targets of the Xenopus T-box gene Xbra
AU - Saka, Yasushi
AU - Tada, M
AU - Smith, J C
PY - 2000/5/1
Y1 - 2000/5/1
N2 - Brachyury (T), a member of the T-box gene family, is essential for the formation of posterior mesoderm and notochord in vertebrate development. Expression of the Xenopus homologue of Brachyury, Xbra, causes ectopic ventral and lateral mesoderm formation in animal cap explants and co-expression of Xbra with Pintallavis, a forkhead/HNF3beta-related transcription factor, induces notochord. Although eFGF and the Bix genes are thought to be direct targets of Xbra, no other target genes have been identified. Here, we describe the use of hormone-inducible versions of Xbra and Pintallavis to construct cDNA libraries enriched for targets of these transcription factors. Five putative targets were isolated: Xwnt11, the homeobox gene Bix1, the zinc-finger transcription factor Xegr-1, a putative homologue of the antiproliferative gene BTG1 called Xbtg1, and BIG3/1A11, a gene of unknown function. Expression of Xegr-1 and Xbtg1 is controlled by Pintallavis alone as well as by a combination of Xbra and Pintallavis. Overexpression of Xbtg1 perturbed gastrulation and caused defects in posterior tissues and in notochord and muscle formation, a phenotype reminiscent of that observed with a dominant-negative version of Pintallavis called Pintallavis-En(R). The Brachyury-inducible genes we have isolated shed light on the mechanism of Brachyury function during mesoderm formation. Specification of mesodermal cells is regulated by targets including Bix1-4 and eFGF, while gastrulation movements and perhaps cell division are regulated by Xwnt11 and Xbtg1.
AB - Brachyury (T), a member of the T-box gene family, is essential for the formation of posterior mesoderm and notochord in vertebrate development. Expression of the Xenopus homologue of Brachyury, Xbra, causes ectopic ventral and lateral mesoderm formation in animal cap explants and co-expression of Xbra with Pintallavis, a forkhead/HNF3beta-related transcription factor, induces notochord. Although eFGF and the Bix genes are thought to be direct targets of Xbra, no other target genes have been identified. Here, we describe the use of hormone-inducible versions of Xbra and Pintallavis to construct cDNA libraries enriched for targets of these transcription factors. Five putative targets were isolated: Xwnt11, the homeobox gene Bix1, the zinc-finger transcription factor Xegr-1, a putative homologue of the antiproliferative gene BTG1 called Xbtg1, and BIG3/1A11, a gene of unknown function. Expression of Xegr-1 and Xbtg1 is controlled by Pintallavis alone as well as by a combination of Xbra and Pintallavis. Overexpression of Xbtg1 perturbed gastrulation and caused defects in posterior tissues and in notochord and muscle formation, a phenotype reminiscent of that observed with a dominant-negative version of Pintallavis called Pintallavis-En(R). The Brachyury-inducible genes we have isolated shed light on the mechanism of Brachyury function during mesoderm formation. Specification of mesodermal cells is regulated by targets including Bix1-4 and eFGF, while gastrulation movements and perhaps cell division are regulated by Xwnt11 and Xbtg1.
KW - Amino Acid Sequence
KW - Animals
KW - Base Sequence
KW - Cattle
KW - DNA, Complementary
KW - DNA-Binding Proteins
KW - Dexamethasone
KW - Early Growth Response Protein 1
KW - Gastrula
KW - Gene Expression Regulation, Developmental
KW - Genes, Tumor Suppressor
KW - Glycoproteins
KW - Homeodomain Proteins
KW - Humans
KW - Immediate-Early Proteins
KW - Mesoderm
KW - Molecular Sequence Data
KW - Neoplasm Proteins
KW - Recombinant Fusion Proteins
KW - T-Box Domain Proteins
KW - Trans-Activators
KW - Transcription Factors
KW - Wnt Proteins
KW - Xenopus
KW - Xenopus Proteins
KW - Brachyury
KW - Xbra
KW - Pintallavis
KW - Xenopus laevis
U2 - 10.1016/S0925-4773(00)00260-4
DO - 10.1016/S0925-4773(00)00260-4
M3 - Article
VL - 93
SP - 27
EP - 39
JO - Mechanisms of Development
JF - Mechanisms of Development
SN - 0925-4773
IS - 1-2
ER -