A sensitive microsphere coagulation ELISA for Escherichia coli O157 : H7 using Russell's viper venom

N J C Strachan, I D Ogden

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11 Citations (Scopus)

Abstract

A microsphere coagulation enzyme-linked immunosorbent assay (MC-ELISA) using Russell's viper venom factor X activator (RVV-XA) is described for the detection of Escherichia coli O157:H7. This microtitre plate assay comprises a standard sandwich immunoassay incorporating RVV-XA as the enzyme label. Coagulation substrate together with polystyrene microspheres are added to the wells of the microtitre plate. RVV-XA initiates the coagulation cascade causing formation of an artificial clot of polystyrene microspheres bound together with fibrin. As few as 10(2) E. coli O157 in a well (10(3) per ml) can be detected within 3 h. The assay is two orders of magnitude more sensitive than a standard ELISA and is a generic technique with the potential for widespread use in sandwich immunoassays. (C) 2000 Published by Elsevier Science B.V. All rights reserved.

Original languageEnglish
Pages (from-to)79-84
Number of pages6
JournalFEMS Microbiology Letters
Volume186
DOIs
Publication statusPublished - 2000

Keywords

  • enzyme-linked immunosorbent assay
  • Russell's viper venom
  • Escherichia coli O157 : H7
  • HEMOLYTIC-UREMIC SYNDROME
  • ASSAY
  • OUTBREAK
  • SAMPLES
  • FOODS

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