A stable bioluminescent construct of Escherichia coli O157:H7 for hazard assessments of long-term survival in the environment.

J. M. Ritchie, G. R. Campbell, J. Shepherd, Y. Beaton, D. Jones, Kenneth Stuart Killham, Rebekka Artz

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

A chromosomally lux-marked (Tn5 luxCDABE) strain of nontoxigenic Escherichia coli O157:H7 was constructed by transposon mutagenesis and shown to have retained the O157, H7, and intimin phenotypes. The survival characteristics of this strain in the experiments performed (soil at -5, -100, and -1,500 kPa matric potential and artificial groundwater) were indistinguishable from the wild-type strain. Evaluation of potential luminescence was found to be a rapid, cheap, and quantitative measure of viable E. coli O157:H7 Tn5 luxCDABE populations in environmental samples. In the survival studies, bioluminescence of the starved populations of E. coli O157:H7 Tn5 luxCDABE could be reactivated to the original levels of light emission, suggesting that these populations remain viable and potentially infective to humans. The attributes of the construct offer a cheap and low-risk substitute to the use of verocytotoxin-producing E. coli O157:H7 in long-term survival studies.

Original languageEnglish
Pages (from-to)3359-3367
Number of pages8
JournalApplied and Environmental Microbiology
Volume69
DOIs
Publication statusPublished - 2003

Keywords

  • MODIFIED PSEUDOMONAS-FLUORESCENS
  • HEMOLYTIC-UREMIC SYNDROME
  • SHIGA TOXIN
  • NONCULTURABLE STATE
  • SOIL
  • LUMINESCENCE
  • BACTERIA
  • WATER
  • STARVATION
  • POPULATION

Cite this

A stable bioluminescent construct of Escherichia coli O157:H7 for hazard assessments of long-term survival in the environment. / Ritchie, J. M.; Campbell, G. R.; Shepherd, J.; Beaton, Y.; Jones, D.; Killham, Kenneth Stuart; Artz, Rebekka.

In: Applied and Environmental Microbiology, Vol. 69, 2003, p. 3359-3367.

Research output: Contribution to journalArticle

Ritchie, J. M. ; Campbell, G. R. ; Shepherd, J. ; Beaton, Y. ; Jones, D. ; Killham, Kenneth Stuart ; Artz, Rebekka. / A stable bioluminescent construct of Escherichia coli O157:H7 for hazard assessments of long-term survival in the environment. In: Applied and Environmental Microbiology. 2003 ; Vol. 69. pp. 3359-3367.
@article{99e84115d5ad4320bc8072e8a9c7ba79,
title = "A stable bioluminescent construct of Escherichia coli O157:H7 for hazard assessments of long-term survival in the environment.",
abstract = "A chromosomally lux-marked (Tn5 luxCDABE) strain of nontoxigenic Escherichia coli O157:H7 was constructed by transposon mutagenesis and shown to have retained the O157, H7, and intimin phenotypes. The survival characteristics of this strain in the experiments performed (soil at -5, -100, and -1,500 kPa matric potential and artificial groundwater) were indistinguishable from the wild-type strain. Evaluation of potential luminescence was found to be a rapid, cheap, and quantitative measure of viable E. coli O157:H7 Tn5 luxCDABE populations in environmental samples. In the survival studies, bioluminescence of the starved populations of E. coli O157:H7 Tn5 luxCDABE could be reactivated to the original levels of light emission, suggesting that these populations remain viable and potentially infective to humans. The attributes of the construct offer a cheap and low-risk substitute to the use of verocytotoxin-producing E. coli O157:H7 in long-term survival studies.",
keywords = "MODIFIED PSEUDOMONAS-FLUORESCENS, HEMOLYTIC-UREMIC SYNDROME, SHIGA TOXIN, NONCULTURABLE STATE, SOIL, LUMINESCENCE, BACTERIA, WATER, STARVATION, POPULATION",
author = "Ritchie, {J. M.} and Campbell, {G. R.} and J. Shepherd and Y. Beaton and D. Jones and Killham, {Kenneth Stuart} and Rebekka Artz",
year = "2003",
doi = "10.1128/AEM.69.6.3359-3367.2003",
language = "English",
volume = "69",
pages = "3359--3367",
journal = "Applied and Environmental Microbiology",
issn = "0099-2240",
publisher = "American Society for Microbiology",

}

TY - JOUR

T1 - A stable bioluminescent construct of Escherichia coli O157:H7 for hazard assessments of long-term survival in the environment.

AU - Ritchie, J. M.

AU - Campbell, G. R.

AU - Shepherd, J.

AU - Beaton, Y.

AU - Jones, D.

AU - Killham, Kenneth Stuart

AU - Artz, Rebekka

PY - 2003

Y1 - 2003

N2 - A chromosomally lux-marked (Tn5 luxCDABE) strain of nontoxigenic Escherichia coli O157:H7 was constructed by transposon mutagenesis and shown to have retained the O157, H7, and intimin phenotypes. The survival characteristics of this strain in the experiments performed (soil at -5, -100, and -1,500 kPa matric potential and artificial groundwater) were indistinguishable from the wild-type strain. Evaluation of potential luminescence was found to be a rapid, cheap, and quantitative measure of viable E. coli O157:H7 Tn5 luxCDABE populations in environmental samples. In the survival studies, bioluminescence of the starved populations of E. coli O157:H7 Tn5 luxCDABE could be reactivated to the original levels of light emission, suggesting that these populations remain viable and potentially infective to humans. The attributes of the construct offer a cheap and low-risk substitute to the use of verocytotoxin-producing E. coli O157:H7 in long-term survival studies.

AB - A chromosomally lux-marked (Tn5 luxCDABE) strain of nontoxigenic Escherichia coli O157:H7 was constructed by transposon mutagenesis and shown to have retained the O157, H7, and intimin phenotypes. The survival characteristics of this strain in the experiments performed (soil at -5, -100, and -1,500 kPa matric potential and artificial groundwater) were indistinguishable from the wild-type strain. Evaluation of potential luminescence was found to be a rapid, cheap, and quantitative measure of viable E. coli O157:H7 Tn5 luxCDABE populations in environmental samples. In the survival studies, bioluminescence of the starved populations of E. coli O157:H7 Tn5 luxCDABE could be reactivated to the original levels of light emission, suggesting that these populations remain viable and potentially infective to humans. The attributes of the construct offer a cheap and low-risk substitute to the use of verocytotoxin-producing E. coli O157:H7 in long-term survival studies.

KW - MODIFIED PSEUDOMONAS-FLUORESCENS

KW - HEMOLYTIC-UREMIC SYNDROME

KW - SHIGA TOXIN

KW - NONCULTURABLE STATE

KW - SOIL

KW - LUMINESCENCE

KW - BACTERIA

KW - WATER

KW - STARVATION

KW - POPULATION

U2 - 10.1128/AEM.69.6.3359-3367.2003

DO - 10.1128/AEM.69.6.3359-3367.2003

M3 - Article

VL - 69

SP - 3359

EP - 3367

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

ER -