Abstract
Polysaccharide-degrading enzymes of the anaerobic rumen bacterium Ruminococcus flavefaciens strain 17 were exmined. This work demonstrated that R. flavefaciens strain 17 produces a complex array of enzymes active against cellulose, hemicellulose (xylan) and lignin. These enzymes are xylanase, avicellase, acid-swollen cellulase, carboxymethyl cellulase (CMCase), pNP-cellobiosidase (pNPC), pNP-glucopyranosidase (pNPG), pNP-xylopyranosidase (pNPX) and pNP-arabinofuranosidase (pNPA). Although R. flavefaciens, is an anaerobic bacterium, its enzymes worked optimally under aerobic conditions and with dithiothreitol (DTT), except in the case of pNP-xylopyranosidase. pNP-xylopyranosidase exhibited a higher level of activity under anaerobic conditions with DTT. It was demonstrated that the cross-links between arabinose side chains, which esterified via ferulic acid and p-coumaric acid, were cleaved by R. flavefaciens esterase and alpha -arabinofuranosidase.
Original language | English |
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Pages (from-to) | 703-709 |
Number of pages | 7 |
Journal | Turkish Journal of Veterinary & Animal Sciences |
Volume | 25 |
Issue number | 5 |
Publication status | Published - 2001 |
Keywords
- Ruminococcus flavefaciens
- polysaccharidase
- esterase
- enzymes
- PLANT-CELL WALLS
- CONTINUOUS CULTURE
- DEGRADING ENZYMES
- PURE CULTURES
- XYLANASE
- RUMINOCOCCUS-FLAVEFACIENS-17
- FERMENTATION
- DEGRADATION
- EXPRESSION
- GROWTH