A study on cellulolytic and hemicellulolytic enzymes of anaerobic rumen bacterium Ruminococcus flavefaciens strain 17

M S Ekinci, N Ozcan, E Ozkose, H J Flint

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Abstract

Polysaccharide-degrading enzymes of the anaerobic rumen bacterium Ruminococcus flavefaciens strain 17 were exmined. This work demonstrated that R. flavefaciens strain 17 produces a complex array of enzymes active against cellulose, hemicellulose (xylan) and lignin. These enzymes are xylanase, avicellase, acid-swollen cellulase, carboxymethyl cellulase (CMCase), pNP-cellobiosidase (pNPC), pNP-glucopyranosidase (pNPG), pNP-xylopyranosidase (pNPX) and pNP-arabinofuranosidase (pNPA). Although R. flavefaciens, is an anaerobic bacterium, its enzymes worked optimally under aerobic conditions and with dithiothreitol (DTT), except in the case of pNP-xylopyranosidase. pNP-xylopyranosidase exhibited a higher level of activity under anaerobic conditions with DTT. It was demonstrated that the cross-links between arabinose side chains, which esterified via ferulic acid and p-coumaric acid, were cleaved by R. flavefaciens esterase and alpha -arabinofuranosidase.

Original languageEnglish
Pages (from-to)703-709
Number of pages7
JournalTurkish Journal of Veterinary & Animal Sciences
Volume25
Issue number5
Publication statusPublished - 2001

Keywords

  • Ruminococcus flavefaciens
  • polysaccharidase
  • esterase
  • enzymes
  • PLANT-CELL WALLS
  • CONTINUOUS CULTURE
  • DEGRADING ENZYMES
  • PURE CULTURES
  • XYLANASE
  • RUMINOCOCCUS-FLAVEFACIENS-17
  • FERMENTATION
  • DEGRADATION
  • EXPRESSION
  • GROWTH

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