The investigation of benthic biodiversity and biogeochemical processes in the deep-sea is complicated by the need to conduct experiments at in situ pressures. Recovery of sediment samples to the surface without maintaining full-depth ambient pressure may damage the organisms that are of interest or cause physiological changes which could influence the processes being studied. It is possible to carry out in situ experiments using remotely operated vehicles (ROVs) or lander systems. However, the costs and complexity of ROV operations are significant and, for both ROVs and landers, the complexity and repeatability of the experiments are subject to the limitations imposed by these platforms. A system is described (the Multi-Autoclave Corer Experiment – MAC-EXP) which has been developed with the aim of offering a new experimental approach to investigators. The MAC-EXP system is designed to retrieve sediment cores from depths down to 3500m and to seal them into pressure chambers before being recovered so that they are maintained at their normal ambient pressure. After recovery the core chambers can be connected to a laboratory incubation system which allows for experimentation on the sediment without loss of pressure and under controlled conditions of temperature and oxygen concentration. The system is relatively low cost when compared to ROV systems and can be deployed using methods and equipment similar to those used for routine deployments of small unpressurized multi-corers. The results of sea trials are detailed.