A TLR9 homolog that is up-regulated by IFN-gamma in Atlantic salmon (Salmo salar)

Ingrid Skjaeveland; Dimitar B. Iliev; Jun Zou; Trond Jorgensen; Jorunn B. Jorgensen

doi:10.1016/j.dci.2007.10.011

A TLR9 homolog that is up-regulated by IFN-gamma in Atlantic salmon (Salmo salar)

Ingrid Skjaeveland, Dimitar B. Iliev, Jun Zou, Trond Jorgensen, Jorunn B. Jorgensen

Norwegian Coll Fishery Sci

Research output: Contribution to journal › Article › peer-review

69 Citations (Scopus)

Abstract

Members of the Toll-like receptor family 9 (TLR9) subfamily sense viral and bacterial DNA present in the endosomal compartment. Here we describe the cloning and regulation of a TLR9 gene from Atlantic salmon (Salmo salar). The salmon TLR9 cDNA encodes 1075 amino acids and analysis of the inferred protein sequence shows that several amino acid residues known to be important for the functions of TLR9 in mammals are conserved in salmon. Furthermore, TLR9 expression was elevated in head kidney teukocytes after in vitro treatment with CpG ODNs and recombinant trout interferon (IFN)-gamma. IFN-gamma was the strongest inducer of TLR9 expression. Together, the results indicate that the structure, the expression and possibly the function of TLR9 are conserved across the teleost and mammalian lineages. (C) 2007 Elsevier Ltd. All rights reserved.

Original language	English
Pages (from-to)	603-607
Number of pages	5
Journal	Developmental and Comparative Immunology
Volume	32
Issue number	6
Early online date	21 Nov 2007
DOIs	https://doi.org/10.1016/j.dci.2007.10.011
Publication status	Published - 2008

Keywords

TLR9
Atlantic salmon
innate immunity
CpG
IFN-gamma
toll-like receptor
trout oncorhynchus-mykiss
CPG-DNA
expression analysis
gene family
sequence
oligodeoxynucleotides
toll-like-receptor-9
macrophages
cells

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10.1016/j.dci.2007.10.011

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title = "A TLR9 homolog that is up-regulated by IFN-gamma in Atlantic salmon (Salmo salar)",

abstract = "Members of the Toll-like receptor family 9 (TLR9) subfamily sense viral and bacterial DNA present in the endosomal compartment. Here we describe the cloning and regulation of a TLR9 gene from Atlantic salmon (Salmo salar). The salmon TLR9 cDNA encodes 1075 amino acids and analysis of the inferred protein sequence shows that several amino acid residues known to be important for the functions of TLR9 in mammals are conserved in salmon. Furthermore, TLR9 expression was elevated in head kidney teukocytes after in vitro treatment with CpG ODNs and recombinant trout interferon (IFN)-gamma. IFN-gamma was the strongest inducer of TLR9 expression. Together, the results indicate that the structure, the expression and possibly the function of TLR9 are conserved across the teleost and mammalian lineages. (C) 2007 Elsevier Ltd. All rights reserved.",

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author = "Ingrid Skjaeveland and Iliev, {Dimitar B.} and Jun Zou and Trond Jorgensen and Jorgensen, {Jorunn B.}",

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T1 - A TLR9 homolog that is up-regulated by IFN-gamma in Atlantic salmon (Salmo salar)

AU - Skjaeveland, Ingrid

AU - Iliev, Dimitar B.

AU - Zou, Jun

AU - Jorgensen, Trond

AU - Jorgensen, Jorunn B.

PY - 2008

Y1 - 2008

N2 - Members of the Toll-like receptor family 9 (TLR9) subfamily sense viral and bacterial DNA present in the endosomal compartment. Here we describe the cloning and regulation of a TLR9 gene from Atlantic salmon (Salmo salar). The salmon TLR9 cDNA encodes 1075 amino acids and analysis of the inferred protein sequence shows that several amino acid residues known to be important for the functions of TLR9 in mammals are conserved in salmon. Furthermore, TLR9 expression was elevated in head kidney teukocytes after in vitro treatment with CpG ODNs and recombinant trout interferon (IFN)-gamma. IFN-gamma was the strongest inducer of TLR9 expression. Together, the results indicate that the structure, the expression and possibly the function of TLR9 are conserved across the teleost and mammalian lineages. (C) 2007 Elsevier Ltd. All rights reserved.

AB - Members of the Toll-like receptor family 9 (TLR9) subfamily sense viral and bacterial DNA present in the endosomal compartment. Here we describe the cloning and regulation of a TLR9 gene from Atlantic salmon (Salmo salar). The salmon TLR9 cDNA encodes 1075 amino acids and analysis of the inferred protein sequence shows that several amino acid residues known to be important for the functions of TLR9 in mammals are conserved in salmon. Furthermore, TLR9 expression was elevated in head kidney teukocytes after in vitro treatment with CpG ODNs and recombinant trout interferon (IFN)-gamma. IFN-gamma was the strongest inducer of TLR9 expression. Together, the results indicate that the structure, the expression and possibly the function of TLR9 are conserved across the teleost and mammalian lineages. (C) 2007 Elsevier Ltd. All rights reserved.

KW - TLR9

KW - Atlantic salmon

KW - innate immunity

KW - CpG

KW - IFN-gamma

KW - toll-like receptor

KW - trout oncorhynchus-mykiss

KW - CPG-DNA

KW - expression analysis

KW - gene family

KW - sequence

KW - oligodeoxynucleotides

KW - toll-like-receptor-9

KW - macrophages

KW - cells

U2 - 10.1016/j.dci.2007.10.011

DO - 10.1016/j.dci.2007.10.011

M3 - Article

SN - 0145-305X

VL - 32

SP - 603

EP - 607

JO - Developmental and Comparative Immunology

JF - Developmental and Comparative Immunology

IS - 6

ER -

A TLR9 homolog that is up-regulated by IFN-gamma in Atlantic salmon (Salmo salar)

Abstract

Keywords

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