Abstract
Plant-derived carbon is the substrate which drives the rate of microbial assimilation and turnover of nutrients, in particular N and P, within the rhizosphere. To develop a better understanding of rhizosphere dynamics. a tripartite reporter gene system has been developed. We used three lux-marked Pseudomonas fluorescens strains to report on soil (1) assimilable carbon, (2) N-status, and (3) P-status. In vivo studies using soil water. spiked with C. N and P to simulate rhizosphere conditions, showed that the tripartite reporter system can provide real-time assessment of carbon and nutrient status. Good quantitative agreement for bioluminescence output between reference material and soil water samples was found for the C and P reporters. With regard to soil nitrate, the minimum bioavailable concentration was found to be greater than that analytically detectable in soil water. This is the First time that bioavailable soil C, N and P have been quantified using a tripartite reporter gene system. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
Original language | English |
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Pages (from-to) | 35-39 |
Number of pages | 4 |
Journal | FEMS Microbiology Ecology |
Volume | 220 |
DOIs | |
Publication status | Published - 2003 |
Keywords
- Pseudomonas fluorescens DF57 N3
- P. fluorescens DF57 P9
- reporter gene
- bio-available nutrients
- PSEUDOMONAS-FLUORESCENS DF57
- BARLEY RHIZOSPHERE
- NITROGEN
- STARVATION