Abstract
Plant-derived carbon is the substrate which drives the rate of microbial assimilation and turnover of nutrients, in particular N and P, within the rhizosphere. To develop a better understanding of rhizosphere dynamics. a tripartite reporter gene system has been developed. We used three lux-marked Pseudomonas fluorescens strains to report on soil (1) assimilable carbon, (2) N-status, and (3) P-status. In vivo studies using soil water. spiked with C. N and P to simulate rhizosphere conditions, showed that the tripartite reporter system can provide real-time assessment of carbon and nutrient status. Good quantitative agreement for bioluminescence output between reference material and soil water samples was found for the C and P reporters. With regard to soil nitrate, the minimum bioavailable concentration was found to be greater than that analytically detectable in soil water. This is the First time that bioavailable soil C, N and P have been quantified using a tripartite reporter gene system. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
| Original language | English |
|---|---|
| Pages (from-to) | 35-39 |
| Number of pages | 4 |
| Journal | FEMS Microbiology Ecology |
| Volume | 220 |
| DOIs | |
| Publication status | Published - 2003 |
Keywords
- Pseudomonas fluorescens DF57 N3
- P. fluorescens DF57 P9
- reporter gene
- bio-available nutrients
- PSEUDOMONAS-FLUORESCENS DF57
- BARLEY RHIZOSPHERE
- NITROGEN
- STARVATION
Cite this
A tripartite microbial reporter gene system for real-time assays of soil nutrient status. / Meharg, Andrew Alexander.
In: FEMS Microbiology Ecology, Vol. 220, 2003, p. 35-39.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A tripartite microbial reporter gene system for real-time assays of soil nutrient status.
AU - Meharg, Andrew Alexander
PY - 2003
Y1 - 2003
N2 - Plant-derived carbon is the substrate which drives the rate of microbial assimilation and turnover of nutrients, in particular N and P, within the rhizosphere. To develop a better understanding of rhizosphere dynamics. a tripartite reporter gene system has been developed. We used three lux-marked Pseudomonas fluorescens strains to report on soil (1) assimilable carbon, (2) N-status, and (3) P-status. In vivo studies using soil water. spiked with C. N and P to simulate rhizosphere conditions, showed that the tripartite reporter system can provide real-time assessment of carbon and nutrient status. Good quantitative agreement for bioluminescence output between reference material and soil water samples was found for the C and P reporters. With regard to soil nitrate, the minimum bioavailable concentration was found to be greater than that analytically detectable in soil water. This is the First time that bioavailable soil C, N and P have been quantified using a tripartite reporter gene system. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
AB - Plant-derived carbon is the substrate which drives the rate of microbial assimilation and turnover of nutrients, in particular N and P, within the rhizosphere. To develop a better understanding of rhizosphere dynamics. a tripartite reporter gene system has been developed. We used three lux-marked Pseudomonas fluorescens strains to report on soil (1) assimilable carbon, (2) N-status, and (3) P-status. In vivo studies using soil water. spiked with C. N and P to simulate rhizosphere conditions, showed that the tripartite reporter system can provide real-time assessment of carbon and nutrient status. Good quantitative agreement for bioluminescence output between reference material and soil water samples was found for the C and P reporters. With regard to soil nitrate, the minimum bioavailable concentration was found to be greater than that analytically detectable in soil water. This is the First time that bioavailable soil C, N and P have been quantified using a tripartite reporter gene system. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
KW - Pseudomonas fluorescens DF57 N3
KW - P. fluorescens DF57 P9
KW - reporter gene
KW - bio-available nutrients
KW - PSEUDOMONAS-FLUORESCENS DF57
KW - BARLEY RHIZOSPHERE
KW - NITROGEN
KW - STARVATION
U2 - 10.1016/S0378-1097(03)00057-0
DO - 10.1016/S0378-1097(03)00057-0
M3 - Article
VL - 220
SP - 35
EP - 39
JO - FEMS Microbiology Ecology
JF - FEMS Microbiology Ecology
SN - 1574-6941
ER -