Abstract
A two-step radiolabelling protocol of a cancer relevant cRGD peptide is described where the fluorinase enzyme is used to catalyse a transhalogenation reaction to generate [18F]-5'-fluoro-5'-deoxy-2-ethynyladenosine, [18F]FDEA, followed by a ‘click’ reaction to an azide tethered cRGD peptide. This protocol offers an efficient radiolabelling of a biologically relevant peptide construct in water at pH 7.8, 37oC in 2 hours, that was metabolically stable in rats and retained high affinity for the αVβ3 integrin.
Original language | English |
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Pages (from-to) | 13542-13545 |
Number of pages | 4 |
Journal | Chemical Communications |
Volume | 51 |
Issue number | 70 |
Early online date | 17 Jul 2015 |
DOIs | |
Publication status | Published - 2015 |
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Ian Fleming
- School of Medicine, Medical Sciences & Nutrition, Medical Education - Senior Lecturer (Scholarship)
- School of Medicine, Medical Sciences & Nutrition, Aberdeen Biomedical Imaging Centre
Person: Academic Related - Scholarship