Cyanobactins are a rapidly growing family of linear and cyclic peptides produced by cyanobacteria. Kawaguchipeptins A and B, two macrocyclic undecapeptides reported earlier from Microcystis aeruginosa NIES-88, are shown to be products of the cyanobactin biosynthetic pathway. The 9 kb kawaguchipeptin (kgp) gene cluster was identified in a 5.26 Mb draft genome of Microcystis aeruginosa NIES-88. We verified that this gene cluster is responsible for the production of the kawaguchipeptins through heterologous expression of the kgp gene cluster in Escherichia coli. The KgpF prenyltransferase was overexpressed and was shown to prenylate C-3 of Trp residues in both linear and cyclic peptides in vitro. Our findings serve to further enhance the structural diversity of cyanobactins to include tryptophan-prenylated cyclic peptides.
|Number of pages||4|
|Journal||Angewandte Chemie International Edition|
|Early online date||5 Feb 2016|
|Publication status||Published - 7 Mar 2016|
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Wael E Houssen
- School of Medicine, Medical Sciences & Nutrition, Medical Sciences - Reader
- Institute of Medical Sciences
- School of Natural & Computing Sciences, Chemistry - Chair in Chemistry
- School of Natural & Computing Sciences, Chemistry - Senior Lecturer