A Xenopus tribbles orthologue is required for the progression of mitosis and for development of the nervous system

Yasushi Saka, J C Smith

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The product of the Drosophila gene tribbles inhibits cell division in the ventral furrow of the embryo and thereby allows the normal prosecution of gastrulation. Cell division is also absent in involuting dorsal mesoderm during gastrulation in Xenopus, and to ask whether the two species employ similar mechanisms to coordinate morphogenesis and the cell cycle, we isolated a putative Xenopus homologue of tribbles which we call Xtrb2. Extensive cDNA cloning identified long and short forms of Xtrb2, termed Xtrb2-L and Xtrb2-S, respectively. Xtrb2 is expressed maternally and in mesoderm and ectoderm at blastula and gastrula stages. Later, it is expressed in dorsal neural tube, eyes, and cephalic neural crest. Time-lapse imaging of GFP-tagged Xtrb2-L suggests that during cell division, it is associated with mitotic spindles. Knockdown of Xtrb2 by antisense morpholino oligonucleotides (MOs) disrupted synchronous cell divisions during blastula stages, apparently as a result of delayed progression through mitosis and cytokinesis. At later stages, tissues expressing the highest levels of Xtrb2 were most markedly affected by morpholino knockdown, with perturbation of neural crest and eye development.
Original languageEnglish
Pages (from-to)210-225
Number of pages16
JournalDevelopmental Biology
Volume273
Issue number2
Early online date20 Jul 2004
DOIs
Publication statusPublished - 15 Sep 2004

Fingerprint

Xenopus
Mitosis
Cell Division
Nervous System
Blastula
Morpholinos
Gastrulation
Neural Crest
Mesoderm
Time-Lapse Imaging
Gastrula
Ectoderm
Spindle Apparatus
Neural Tube
Cytokinesis
Antisense Oligonucleotides
Morphogenesis
Drosophila
Organism Cloning
Cell Cycle

Keywords

  • Amino Acid Sequence
  • Animals
  • Animals, Genetically Modified
  • Base Sequence
  • Cell Cycle Proteins
  • Cloning, Molecular
  • DNA, Complementary
  • Drosophila Proteins
  • Eye
  • Gene Expression Regulation, Developmental
  • Gene Targeting
  • Mitosis
  • Molecular Sequence Data
  • Nervous System
  • Neural Crest
  • Oligonucleotides, Antisense
  • Phylogeny
  • Protein-Serine-Threonine Kinases
  • Sequence Homology, Amino Acid
  • Sequence Homology, Nucleic Acid
  • Xenopus Proteins
  • Xenopus laevis
  • Xenopus
  • cell cycle
  • mitosis
  • tribbles
  • neural crest

Cite this

A Xenopus tribbles orthologue is required for the progression of mitosis and for development of the nervous system. / Saka, Yasushi; Smith, J C.

In: Developmental Biology, Vol. 273, No. 2, 15.09.2004, p. 210-225.

Research output: Contribution to journalArticle

@article{102f55122cc241f99e5d8cfcf3ae1044,
title = "A Xenopus tribbles orthologue is required for the progression of mitosis and for development of the nervous system",
abstract = "The product of the Drosophila gene tribbles inhibits cell division in the ventral furrow of the embryo and thereby allows the normal prosecution of gastrulation. Cell division is also absent in involuting dorsal mesoderm during gastrulation in Xenopus, and to ask whether the two species employ similar mechanisms to coordinate morphogenesis and the cell cycle, we isolated a putative Xenopus homologue of tribbles which we call Xtrb2. Extensive cDNA cloning identified long and short forms of Xtrb2, termed Xtrb2-L and Xtrb2-S, respectively. Xtrb2 is expressed maternally and in mesoderm and ectoderm at blastula and gastrula stages. Later, it is expressed in dorsal neural tube, eyes, and cephalic neural crest. Time-lapse imaging of GFP-tagged Xtrb2-L suggests that during cell division, it is associated with mitotic spindles. Knockdown of Xtrb2 by antisense morpholino oligonucleotides (MOs) disrupted synchronous cell divisions during blastula stages, apparently as a result of delayed progression through mitosis and cytokinesis. At later stages, tissues expressing the highest levels of Xtrb2 were most markedly affected by morpholino knockdown, with perturbation of neural crest and eye development.",
keywords = "Amino Acid Sequence, Animals, Animals, Genetically Modified, Base Sequence, Cell Cycle Proteins, Cloning, Molecular, DNA, Complementary, Drosophila Proteins, Eye, Gene Expression Regulation, Developmental, Gene Targeting, Mitosis, Molecular Sequence Data, Nervous System, Neural Crest, Oligonucleotides, Antisense, Phylogeny, Protein-Serine-Threonine Kinases, Sequence Homology, Amino Acid, Sequence Homology, Nucleic Acid, Xenopus Proteins, Xenopus laevis, Xenopus, cell cycle, mitosis, tribbles, neural crest",
author = "Yasushi Saka and Smith, {J C}",
year = "2004",
month = "9",
day = "15",
doi = "10.1016/j.ydbio.2004.05.032",
language = "English",
volume = "273",
pages = "210--225",
journal = "Developmental Biology",
issn = "0012-1606",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - A Xenopus tribbles orthologue is required for the progression of mitosis and for development of the nervous system

AU - Saka, Yasushi

AU - Smith, J C

PY - 2004/9/15

Y1 - 2004/9/15

N2 - The product of the Drosophila gene tribbles inhibits cell division in the ventral furrow of the embryo and thereby allows the normal prosecution of gastrulation. Cell division is also absent in involuting dorsal mesoderm during gastrulation in Xenopus, and to ask whether the two species employ similar mechanisms to coordinate morphogenesis and the cell cycle, we isolated a putative Xenopus homologue of tribbles which we call Xtrb2. Extensive cDNA cloning identified long and short forms of Xtrb2, termed Xtrb2-L and Xtrb2-S, respectively. Xtrb2 is expressed maternally and in mesoderm and ectoderm at blastula and gastrula stages. Later, it is expressed in dorsal neural tube, eyes, and cephalic neural crest. Time-lapse imaging of GFP-tagged Xtrb2-L suggests that during cell division, it is associated with mitotic spindles. Knockdown of Xtrb2 by antisense morpholino oligonucleotides (MOs) disrupted synchronous cell divisions during blastula stages, apparently as a result of delayed progression through mitosis and cytokinesis. At later stages, tissues expressing the highest levels of Xtrb2 were most markedly affected by morpholino knockdown, with perturbation of neural crest and eye development.

AB - The product of the Drosophila gene tribbles inhibits cell division in the ventral furrow of the embryo and thereby allows the normal prosecution of gastrulation. Cell division is also absent in involuting dorsal mesoderm during gastrulation in Xenopus, and to ask whether the two species employ similar mechanisms to coordinate morphogenesis and the cell cycle, we isolated a putative Xenopus homologue of tribbles which we call Xtrb2. Extensive cDNA cloning identified long and short forms of Xtrb2, termed Xtrb2-L and Xtrb2-S, respectively. Xtrb2 is expressed maternally and in mesoderm and ectoderm at blastula and gastrula stages. Later, it is expressed in dorsal neural tube, eyes, and cephalic neural crest. Time-lapse imaging of GFP-tagged Xtrb2-L suggests that during cell division, it is associated with mitotic spindles. Knockdown of Xtrb2 by antisense morpholino oligonucleotides (MOs) disrupted synchronous cell divisions during blastula stages, apparently as a result of delayed progression through mitosis and cytokinesis. At later stages, tissues expressing the highest levels of Xtrb2 were most markedly affected by morpholino knockdown, with perturbation of neural crest and eye development.

KW - Amino Acid Sequence

KW - Animals

KW - Animals, Genetically Modified

KW - Base Sequence

KW - Cell Cycle Proteins

KW - Cloning, Molecular

KW - DNA, Complementary

KW - Drosophila Proteins

KW - Eye

KW - Gene Expression Regulation, Developmental

KW - Gene Targeting

KW - Mitosis

KW - Molecular Sequence Data

KW - Nervous System

KW - Neural Crest

KW - Oligonucleotides, Antisense

KW - Phylogeny

KW - Protein-Serine-Threonine Kinases

KW - Sequence Homology, Amino Acid

KW - Sequence Homology, Nucleic Acid

KW - Xenopus Proteins

KW - Xenopus laevis

KW - Xenopus

KW - cell cycle

KW - mitosis

KW - tribbles

KW - neural crest

U2 - 10.1016/j.ydbio.2004.05.032

DO - 10.1016/j.ydbio.2004.05.032

M3 - Article

C2 - 15328008

VL - 273

SP - 210

EP - 225

JO - Developmental Biology

JF - Developmental Biology

SN - 0012-1606

IS - 2

ER -