Abstract
We report for the first time the cloning and characterisation of a protozoal enzyme involved in plant cell wall polysaccharide degradation. A cDNA library was constructed from the ruminal protozoan Polyplastron multivesiculatum and a stable clone expressing xylanase activity was isolated. The encoded enzyme belongs to the glycoside hydrolase family 11, and phylogenetic analysis indicates a closer relationship with catalytic domains from Gram-positive bacteria than the other fibrolytic eukaryotes from the rumen, the anaerobic fungi. (C) 1999 Published by Elsevier Science B.V. All rights reserved.
| Original language | English |
|---|---|
| Pages (from-to) | 145-152 |
| Number of pages | 8 |
| Journal | FEMS Microbiology Letters |
| Volume | 181 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1 Dec 1999 |
Keywords
- rumen
- protozoa
- xylanase
- polyplastron multivesiculatum
- cellulolytic bacteria
- fungi
- gene
- populations
- cellulases
- digestion
- protein
- domains
- enzymes
- sheep
Cite this
Devillard, E., Newbold, C. J., Scott, K. P., Forano, E., Wallace, R. J., Jouany, J. P., & Flint, H. J. (1999). A xylanase produced by the rumen anaerobic protozoan Polyplastron multivesiculatum shows close sequence similarity to family 11 xylanases from Gram-positive bacteria. FEMS Microbiology Letters, 181(1), 145-152. https://doi.org/10.1111/j.1574-6968.1999.tb08837.x