A xylanase produced by the rumen anaerobic protozoan Polyplastron multivesiculatum shows close sequence similarity to family 11 xylanases from Gram-positive bacteria

E  Devillard; C J  Newbold; K P  Scott; E  Forano; R J Wallace; J P  Jouany; H J Flint

doi:10.1111/j.1574-6968.1999.tb08837.x

A xylanase produced by the rumen anaerobic protozoan Polyplastron multivesiculatum shows close sequence similarity to family 11 xylanases from Gram-positive bacteria

E Devillard, C J Newbold, K P Scott, E Forano, R J Wallace, J P Jouany, H J Flint

The Rowett Institute of Nutrition and Health

Research output: Contribution to journal › Article › peer-review

47 Citations (Scopus)

Abstract

We report for the first time the cloning and characterisation of a protozoal enzyme involved in plant cell wall polysaccharide degradation. A cDNA library was constructed from the ruminal protozoan Polyplastron multivesiculatum and a stable clone expressing xylanase activity was isolated. The encoded enzyme belongs to the glycoside hydrolase family 11, and phylogenetic analysis indicates a closer relationship with catalytic domains from Gram-positive bacteria than the other fibrolytic eukaryotes from the rumen, the anaerobic fungi. (C) 1999 Published by Elsevier Science B.V. All rights reserved.

Original language	English
Pages (from-to)	145-152
Number of pages	8
Journal	FEMS Microbiology Letters
Volume	181
Issue number	1
DOIs	https://doi.org/10.1111/j.1574-6968.1999.tb08837.x
Publication status	Published - 1 Dec 1999

Keywords

rumen
protozoa
xylanase
polyplastron multivesiculatum
cellulolytic bacteria
fungi
gene
populations
cellulases
digestion
protein
domains
enzymes
sheep

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10.1111/j.1574-6968.1999.tb08837.xLicence: Unspecified

Cite this

Devillard, E., Newbold, C. J., Scott, K. P., Forano, E., Wallace, R. J., Jouany, J. P., & Flint, H. J. (1999). A xylanase produced by the rumen anaerobic protozoan Polyplastron multivesiculatum shows close sequence similarity to family 11 xylanases from Gram-positive bacteria. FEMS Microbiology Letters, 181(1), 145-152. https://doi.org/10.1111/j.1574-6968.1999.tb08837.x

A xylanase produced by the rumen anaerobic protozoan Polyplastron multivesiculatum shows close sequence similarity to family 11 xylanases from Gram-positive bacteria. / Devillard, E ; Newbold, C J ; Scott, K P ; Forano, E ; Wallace, R J; Jouany, J P ; Flint, H J.

In: FEMS Microbiology Letters, Vol. 181, No. 1, 01.12.1999, p. 145-152.

Research output: Contribution to journal › Article › peer-review

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