Abstract
We report for the first time the cloning and characterisation of a protozoal enzyme involved in plant cell wall polysaccharide degradation. A cDNA library was constructed from the ruminal protozoan Polyplastron multivesiculatum and a stable clone expressing xylanase activity was isolated. The encoded enzyme belongs to the glycoside hydrolase family 11, and phylogenetic analysis indicates a closer relationship with catalytic domains from Gram-positive bacteria than the other fibrolytic eukaryotes from the rumen, the anaerobic fungi. (C) 1999 Published by Elsevier Science B.V. All rights reserved.
Original language | English |
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Pages (from-to) | 145-152 |
Number of pages | 8 |
Journal | FEMS Microbiology Letters |
Volume | 181 |
Issue number | 1 |
DOIs | |
Publication status | Published - 1 Dec 1999 |
Keywords
- rumen
- protozoa
- xylanase
- polyplastron multivesiculatum
- cellulolytic bacteria
- fungi
- gene
- populations
- cellulases
- digestion
- protein
- domains
- enzymes
- sheep