Abstract
We report for the first time the cloning and characterisation of a protozoal enzyme involved in plant cell wall polysaccharide degradation. A cDNA library was constructed from the ruminal protozoan Polyplastron multivesiculatum and a stable clone expressing xylanase activity was isolated. The encoded enzyme belongs to the glycoside hydrolase family 11, and phylogenetic analysis indicates a closer relationship with catalytic domains from Gram-positive bacteria than the other fibrolytic eukaryotes from the rumen, the anaerobic fungi. (C) 1999 Published by Elsevier Science B.V. All rights reserved.
| Original language | English |
|---|---|
| Pages (from-to) | 145-152 |
| Number of pages | 8 |
| Journal | FEMS Microbiology Letters |
| Volume | 181 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1 Dec 1999 |
Keywords
- rumen
- protozoa
- xylanase
- polyplastron multivesiculatum
- cellulolytic bacteria
- fungi
- gene
- populations
- cellulases
- digestion
- protein
- domains
- enzymes
- sheep
Cite this
A xylanase produced by the rumen anaerobic protozoan Polyplastron multivesiculatum shows close sequence similarity to family 11 xylanases from Gram-positive bacteria. / Devillard, E ; Newbold, C J ; Scott, K P ; Forano, E ; Wallace, R J; Jouany, J P ; Flint, H J.
In: FEMS Microbiology Letters, Vol. 181, No. 1, 01.12.1999, p. 145-152.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A xylanase produced by the rumen anaerobic protozoan Polyplastron multivesiculatum shows close sequence similarity to family 11 xylanases from Gram-positive bacteria
AU - Devillard, E
AU - Newbold, C J
AU - Scott, K P
AU - Forano, E
AU - Wallace, R J
AU - Jouany, J P
AU - Flint, H J
PY - 1999/12/1
Y1 - 1999/12/1
N2 - We report for the first time the cloning and characterisation of a protozoal enzyme involved in plant cell wall polysaccharide degradation. A cDNA library was constructed from the ruminal protozoan Polyplastron multivesiculatum and a stable clone expressing xylanase activity was isolated. The encoded enzyme belongs to the glycoside hydrolase family 11, and phylogenetic analysis indicates a closer relationship with catalytic domains from Gram-positive bacteria than the other fibrolytic eukaryotes from the rumen, the anaerobic fungi. (C) 1999 Published by Elsevier Science B.V. All rights reserved.
AB - We report for the first time the cloning and characterisation of a protozoal enzyme involved in plant cell wall polysaccharide degradation. A cDNA library was constructed from the ruminal protozoan Polyplastron multivesiculatum and a stable clone expressing xylanase activity was isolated. The encoded enzyme belongs to the glycoside hydrolase family 11, and phylogenetic analysis indicates a closer relationship with catalytic domains from Gram-positive bacteria than the other fibrolytic eukaryotes from the rumen, the anaerobic fungi. (C) 1999 Published by Elsevier Science B.V. All rights reserved.
KW - rumen
KW - protozoa
KW - xylanase
KW - polyplastron multivesiculatum
KW - cellulolytic bacteria
KW - fungi
KW - gene
KW - populations
KW - cellulases
KW - digestion
KW - protein
KW - domains
KW - enzymes
KW - sheep
U2 - 10.1111/j.1574-6968.1999.tb08837.x
DO - 10.1111/j.1574-6968.1999.tb08837.x
M3 - Article
VL - 181
SP - 145
EP - 152
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
SN - 0378-1097
IS - 1
ER -