A yeast tRNA mutant that causes pseudohyphal growth exhibits reduced rates of CAG codon translation

Alain Kemp, Russell Betney, Luca Ciandrini, Alexandra Carmen Schwenger, M Carmen Romano, Ian Stansfield

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

In Saccharomyces cerevisiae, the SUP70 gene encodes the CAG-decoding tRNAGlnCUG. A mutant allele, sup70-65, induces pseudohyphal growth on rich medium, an inappropriate nitrogen starvation response. This mutant tRNA is also a UAG nonsense suppressor via first base wobble. To investigate the basis of the pseudohyphal phenotype, 10 novel sup70 UAG suppressor alleles were identified, defining positions in the tRNAGlnCUG anticodon stem that restrict first base wobble. However, none conferred pseudohyphal growth, showing altered CUG anticodon presentation cannot itself induce pseudohyphal growth. Northern blot analysis revealed the sup70-65 tRNAGlnCUG is unstable, inefficiently charged, and 80% reduced in its effective concentration. A stochastic model simulation of translation predicted compromised expression of CAG-rich ORFs in the tRNAGlnCUG-depleted sup70-65 mutant. This prediction was validated by demonstrating that luciferase expression in the mutant was 60% reduced by introducing multiple tandem CAG (but not CAA) codons into this ORF. In addition, the sup70-65 pseudohyphal phenotype was partly complemented by overexpressing CAA-decoding tRNAGlnUUG, an inefficient wobble-decoder of CAG. We thus show that introducing codons decoded by a rare tRNA near the 5' end of an ORF can reduce eukaryote translational expression, and that the mutant tRNACUGGln constitutive pseudohyphal differentiation phenotype correlates strongly with reduced CAG decoding efficiency.
Original languageEnglish
Pages (from-to)284–300
Number of pages27
JournalMolecular Microbiology
Volume87
Issue number2
Early online date4 Dec 2012
DOIs
Publication statusPublished - Jan 2013

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Transfer RNA
Codon
Open Reading Frames
Anticodon
Yeasts
Phenotype
Growth
Alleles
Starvation
Eukaryota
Luciferases
Northern Blotting
Saccharomyces cerevisiae
Nitrogen
Genes

Cite this

A yeast tRNA mutant that causes pseudohyphal growth exhibits reduced rates of CAG codon translation. / Kemp, Alain; Betney, Russell; Ciandrini, Luca; Schwenger, Alexandra Carmen; Romano, M Carmen; Stansfield, Ian.

In: Molecular Microbiology, Vol. 87, No. 2, 01.2013, p. 284–300.

Research output: Contribution to journalArticle

Kemp, Alain ; Betney, Russell ; Ciandrini, Luca ; Schwenger, Alexandra Carmen ; Romano, M Carmen ; Stansfield, Ian. / A yeast tRNA mutant that causes pseudohyphal growth exhibits reduced rates of CAG codon translation. In: Molecular Microbiology. 2013 ; Vol. 87, No. 2. pp. 284–300.
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